Advanced

Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans

Martinez, Alejandra LU ; Delgado, Osvaldo LU ; Baigori, M D and Sineriz, F (2005) In Biotechnology Letters 27(8). p.545-550
Abstract
The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1).
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
purification, Bacillus halodurans MIR32, over-expression, sequence, xylanase
in
Biotechnology Letters
volume
27
issue
8
pages
545 - 550
publisher
Springer
external identifiers
  • pmid:15973487
  • wos:000231955300003
  • scopus:20544471907
ISSN
1573-6776
DOI
10.1007/s10529-005-2879-2
language
English
LU publication?
yes
id
2ecb9584-4441-4fe5-8fbc-1b0c1d91141a (old id 224637)
date added to LUP
2007-10-05 09:05:10
date last changed
2017-08-27 05:22:30
@article{2ecb9584-4441-4fe5-8fbc-1b0c1d91141a,
  abstract     = {The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1).},
  author       = {Martinez, Alejandra and Delgado, Osvaldo and Baigori, M D and Sineriz, F},
  issn         = {1573-6776},
  keyword      = {purification,Bacillus halodurans MIR32,over-expression,sequence,xylanase},
  language     = {eng},
  number       = {8},
  pages        = {545--550},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans},
  url          = {http://dx.doi.org/10.1007/s10529-005-2879-2},
  volume       = {27},
  year         = {2005},
}