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Preliminary evaluation of two fluorescence imaging methods for the detection and the delineation of basal cell carcinomas of the skin

Andersson-Engels, Stefan LU ; Canti, Gianfranco; Cubeddu, Rinaldo; Eker, Charlotta; af Klinteberg, Claes; Pifferi, Antonio; Svanberg, Katarina LU ; Svanberg, Sune LU ; Taroni, Paola and Valentini, Gianluca, et al. (2000) In Lasers in Surgery and Medicine 26(1). p.76-82
Abstract
Abstract Background and ObjectiveFluorescence techniques can provide powerful noninvasive means for medical diagnosis, based on the detection of either endogenous or exogenous fluorophores. The fluorescence of δ-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) has already shown promise for the diagnosis of tumors. The aim of the study was to investigate the localization of skin tumors after the topical application of ALA, by detecting the PpIX fluorescence either in the spectral or in the time domain.Study Design/Materials and MethodsTwo fluorescence imaging systems were used to identify basal cell carcinomas of the skin in humans, after topical application of 20% ALA ointment. Both systems rely on the comparison between the... (More)
Abstract Background and ObjectiveFluorescence techniques can provide powerful noninvasive means for medical diagnosis, based on the detection of either endogenous or exogenous fluorophores. The fluorescence of δ-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) has already shown promise for the diagnosis of tumors. The aim of the study was to investigate the localization of skin tumors after the topical application of ALA, by detecting the PpIX fluorescence either in the spectral or in the time domain.Study Design/Materials and MethodsTwo fluorescence imaging systems were used to identify basal cell carcinomas of the skin in humans, after topical application of 20% ALA ointment. Both systems rely on the comparison between the exogenous and the endogenous fluorescence, performed either in the spectral domain or in the time domain. The first system works by using three images acquired through different spectral filters, whereas the second one measures the spatial map of the average fluorescence lifetime of the sample.ResultsA clear demarcation of skin malignancies was successfully performed in vivo noninvasively with both fluorescence imaging systems.ConclusionThe two complementary approaches considered in the present study show promise for skin tumor detection and delineation based on specific fluorescence features. Lasers Surg. Med. 26:76–82, 2000. © 2000 Wiley-Liss, Inc. (Less)
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publication status
published
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keywords
basal cell carcinoma, δ-aminolevulinic acid, fluorescence imaging, protoporphyrin IX, tumor detection
in
Lasers in Surgery and Medicine
volume
26
issue
1
pages
76 - 82
publisher
John Wiley & Sons
external identifiers
  • scopus:0033957946
ISSN
0196-8092
DOI
10.1002/(SICI)1096-9101(2000)26:1<76::AID-LSM11>3.0.CO;2-4
language
English
LU publication?
yes
id
6b06f28d-f723-491f-9e1e-9d34b35dd65c (old id 2257279)
date added to LUP
2012-02-24 00:18:08
date last changed
2017-08-20 04:30:25
@article{6b06f28d-f723-491f-9e1e-9d34b35dd65c,
  abstract     = {Abstract Background and ObjectiveFluorescence techniques can provide powerful noninvasive means for medical diagnosis, based on the detection of either endogenous or exogenous fluorophores. The fluorescence of δ-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) has already shown promise for the diagnosis of tumors. The aim of the study was to investigate the localization of skin tumors after the topical application of ALA, by detecting the PpIX fluorescence either in the spectral or in the time domain.Study Design/Materials and MethodsTwo fluorescence imaging systems were used to identify basal cell carcinomas of the skin in humans, after topical application of 20% ALA ointment. Both systems rely on the comparison between the exogenous and the endogenous fluorescence, performed either in the spectral domain or in the time domain. The first system works by using three images acquired through different spectral filters, whereas the second one measures the spatial map of the average fluorescence lifetime of the sample.ResultsA clear demarcation of skin malignancies was successfully performed in vivo noninvasively with both fluorescence imaging systems.ConclusionThe two complementary approaches considered in the present study show promise for skin tumor detection and delineation based on specific fluorescence features. Lasers Surg. Med. 26:76–82, 2000. © 2000 Wiley-Liss, Inc.},
  author       = {Andersson-Engels, Stefan and Canti, Gianfranco and Cubeddu, Rinaldo and Eker, Charlotta and af Klinteberg, Claes and Pifferi, Antonio and Svanberg, Katarina and Svanberg, Sune and Taroni, Paola and Valentini, Gianluca and Wang, Ingrid},
  issn         = {0196-8092},
  keyword      = {basal cell carcinoma,δ-aminolevulinic acid,fluorescence imaging,protoporphyrin IX,tumor detection},
  language     = {eng},
  number       = {1},
  pages        = {76--82},
  publisher    = {John Wiley & Sons},
  series       = {Lasers in Surgery and Medicine},
  title        = {Preliminary evaluation of two fluorescence imaging methods for the detection and the delineation of basal cell carcinomas of the skin},
  url          = {http://dx.doi.org/10.1002/(SICI)1096-9101(2000)26:1<76::AID-LSM11>3.0.CO;2-4},
  volume       = {26},
  year         = {2000},
}