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Medical Diagnostic System Based On Simultaneous Multispectral Fluorescence Imaging

Andersson-Engels, Stefan LU ; Johansson, Jonas LU and Svanberg, Sune LU (1994) In Applied Optics 33(34). p.8022-8029
Abstract
A multicolor fluorescence imaging system applied to medical diagnostics is described. The system presented simultaneously records four fluorescence images in different wavelength bands, permitting low-resolution spectroscopy imaging. An arithmetic function image of the four spectral images is constructed by a pixel-to-pixel calculation and is presented on a monitor in false-color coding. A sensitive detector is required for minimizing the excitation energy necessary to obtain an image and thus avoid side effects on the investigated tissue. Characteristics of the system of importance for the detector sensitivity as well as image quality are discussed. A high degree of suppression of ambient background light is reached with this system by... (More)
A multicolor fluorescence imaging system applied to medical diagnostics is described. The system presented simultaneously records four fluorescence images in different wavelength bands, permitting low-resolution spectroscopy imaging. An arithmetic function image of the four spectral images is constructed by a pixel-to-pixel calculation and is presented on a monitor in false-color coding. A sensitive detector is required for minimizing the excitation energy necessary to obtain an image and thus avoid side effects on the investigated tissue. Characteristics of the system of importance for the detector sensitivity as well as image quality are discussed. A high degree of suppression of ambient background light is reached with this system by the use of a pulsed laser as an excitation source together with gated detection. Examples of fluorescence images from tumors on the hind legs and in the brain of rats injected with Photofrin are given. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Optics
volume
33
issue
34
pages
8022 - 8029
publisher
OSA
external identifiers
  • scopus:0028730970
ISSN
2155-3165
DOI
10.1364/AO.33.008022
language
English
LU publication?
yes
id
53a3c4f5-5c7c-4101-b7e5-b62a785e7883 (old id 2257317)
date added to LUP
2012-02-24 09:32:15
date last changed
2017-07-23 04:50:47
@article{53a3c4f5-5c7c-4101-b7e5-b62a785e7883,
  abstract     = {A multicolor fluorescence imaging system applied to medical diagnostics is described. The system presented simultaneously records four fluorescence images in different wavelength bands, permitting low-resolution spectroscopy imaging. An arithmetic function image of the four spectral images is constructed by a pixel-to-pixel calculation and is presented on a monitor in false-color coding. A sensitive detector is required for minimizing the excitation energy necessary to obtain an image and thus avoid side effects on the investigated tissue. Characteristics of the system of importance for the detector sensitivity as well as image quality are discussed. A high degree of suppression of ambient background light is reached with this system by the use of a pulsed laser as an excitation source together with gated detection. Examples of fluorescence images from tumors on the hind legs and in the brain of rats injected with Photofrin are given.},
  author       = {Andersson-Engels, Stefan and Johansson, Jonas and Svanberg, Sune},
  issn         = {2155-3165},
  language     = {eng},
  number       = {34},
  pages        = {8022--8029},
  publisher    = {OSA},
  series       = {Applied Optics},
  title        = {Medical Diagnostic System Based On Simultaneous Multispectral Fluorescence Imaging},
  url          = {http://dx.doi.org/10.1364/AO.33.008022},
  volume       = {33},
  year         = {1994},
}