Time-resolved and Wavelength-resolved Spectroscopy In 2-photon-excited Fluorescence Microscopy
(1994) In Journal of Microscopy 176. p.195-203- Abstract
- Two-photon excited fluorescence spectroscopy has been performed at a microscopic scale in combination with normal, white-light microscopy. This gave simultaneously a spectral resolution of 20 nm and a temporal resolution of 20 ps, from a volume element less than 5 mu m in all three dimensions. The sample was excited with the light from a continuously mode-locked Ti:sapphire laser that was focused on the sample in a fluorescence microscope. A polychromator and a streak-camera were used for detection. The method has been used on tissue, plant and paper samples. It has also been demonstrated how substances naturally occurring in the samples can be identified from their spectroscopic properties and the spatial distribution of these substances... (More)
- Two-photon excited fluorescence spectroscopy has been performed at a microscopic scale in combination with normal, white-light microscopy. This gave simultaneously a spectral resolution of 20 nm and a temporal resolution of 20 ps, from a volume element less than 5 mu m in all three dimensions. The sample was excited with the light from a continuously mode-locked Ti:sapphire laser that was focused on the sample in a fluorescence microscope. A polychromator and a streak-camera were used for detection. The method has been used on tissue, plant and paper samples. It has also been demonstrated how substances naturally occurring in the samples can be identified from their spectroscopic properties and the spatial distribution of these substances can be observed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2257329
- author
- Andersson-Engels, Stefan LU ; Rokahr, I and Carlsson, J
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Microscopy
- volume
- 176
- pages
- 195 - 203
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0028580177
- ISSN
- 0022-2720
- language
- English
- LU publication?
- yes
- id
- f1c26851-b0a5-4d22-b574-eb667cd7d2ea (old id 2257329)
- date added to LUP
- 2016-04-04 09:03:50
- date last changed
- 2025-04-04 14:10:17
@article{f1c26851-b0a5-4d22-b574-eb667cd7d2ea,
abstract = {{Two-photon excited fluorescence spectroscopy has been performed at a microscopic scale in combination with normal, white-light microscopy. This gave simultaneously a spectral resolution of 20 nm and a temporal resolution of 20 ps, from a volume element less than 5 mu m in all three dimensions. The sample was excited with the light from a continuously mode-locked Ti:sapphire laser that was focused on the sample in a fluorescence microscope. A polychromator and a streak-camera were used for detection. The method has been used on tissue, plant and paper samples. It has also been demonstrated how substances naturally occurring in the samples can be identified from their spectroscopic properties and the spatial distribution of these substances can be observed.}},
author = {{Andersson-Engels, Stefan and Rokahr, I and Carlsson, J}},
issn = {{0022-2720}},
language = {{eng}},
pages = {{195--203}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Microscopy}},
title = {{Time-resolved and Wavelength-resolved Spectroscopy In 2-photon-excited Fluorescence Microscopy}},
url = {{https://lup.lub.lu.se/search/files/5221394/2367579.pdf}},
volume = {{176}},
year = {{1994}},
}