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Intra-operative laser-induced photodynamic therapy in the treatment of experimental hepatic tumours

Liu, D. L; Wang, I; Andersson-Engels, Stefan LU ; Hakansson, C. H; Stenram, U and Svanberg, Katarina LU (1995) In European Journal of Gastroenterology and Hepathology 7(11). p.1073-1080
Abstract
Objective: To examine the effect of photodynamic therapy (PDT) on experimental liver tumours in rats. Design: An experimental liver tumour model was used. Each of a group of Fats had two tumours simultaneously inoculated into its liver. The tumour located in the left hepatic robe was used for PDT, and the other one, in the median lobe, as a control. The haem precursor delta-amino laevulinic acid (ALA), at a dose of 30 mg/kg body weight, was injected 60 min before laser irradiation. Rats in group I received ALA through a femoral vein. Those in group II received ALA through the portal vein. Group III had an injection of ALA solution through the portal vein plus hepatic inflow occlusion. Three and 6 days after the treatment, the rats were... (More)
Objective: To examine the effect of photodynamic therapy (PDT) on experimental liver tumours in rats. Design: An experimental liver tumour model was used. Each of a group of Fats had two tumours simultaneously inoculated into its liver. The tumour located in the left hepatic robe was used for PDT, and the other one, in the median lobe, as a control. The haem precursor delta-amino laevulinic acid (ALA), at a dose of 30 mg/kg body weight, was injected 60 min before laser irradiation. Rats in group I received ALA through a femoral vein. Those in group II received ALA through the portal vein. Group III had an injection of ALA solution through the portal vein plus hepatic inflow occlusion. Three and 6 days after the treatment, the rats were killed, and the tumours were measured, and ultrastructural changes were examined using scanning electron microscopy. Setting: Lund University Medical Laser Centre, Lund, Sweden. Results: The mean tumour volume of the treated tumours increased by factors of 1.9, 1.5 and 1.7 in groups I, II and III, respectively, compared with the pretreatment baseline value. However, the mean tumour volume in the control tumours increased by factors of 9.5, 4.3 and 4.8 in the respective groups. Under the light microscope, marked necrosis of the treated tumour and the surrounding liver tissue was observed. Scanning electron microscopy revealed heavy damage to the cells and vessels in the treated tumour. Conclusion: PDT with ALA is an effective treatment modality for rat liver tumours. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Gastroenterology and Hepathology
volume
7
issue
11
pages
1073 - 1080
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0029559815
ISSN
1473-5687
language
English
LU publication?
yes
id
75e7ca16-7882-4f68-a8ca-c88865d092e6 (old id 2258875)
date added to LUP
2012-02-27 21:50:16
date last changed
2017-01-01 07:40:09
@article{75e7ca16-7882-4f68-a8ca-c88865d092e6,
  abstract     = {Objective: To examine the effect of photodynamic therapy (PDT) on experimental liver tumours in rats. Design: An experimental liver tumour model was used. Each of a group of Fats had two tumours simultaneously inoculated into its liver. The tumour located in the left hepatic robe was used for PDT, and the other one, in the median lobe, as a control. The haem precursor delta-amino laevulinic acid (ALA), at a dose of 30 mg/kg body weight, was injected 60 min before laser irradiation. Rats in group I received ALA through a femoral vein. Those in group II received ALA through the portal vein. Group III had an injection of ALA solution through the portal vein plus hepatic inflow occlusion. Three and 6 days after the treatment, the rats were killed, and the tumours were measured, and ultrastructural changes were examined using scanning electron microscopy. Setting: Lund University Medical Laser Centre, Lund, Sweden. Results: The mean tumour volume of the treated tumours increased by factors of 1.9, 1.5 and 1.7 in groups I, II and III, respectively, compared with the pretreatment baseline value. However, the mean tumour volume in the control tumours increased by factors of 9.5, 4.3 and 4.8 in the respective groups. Under the light microscope, marked necrosis of the treated tumour and the surrounding liver tissue was observed. Scanning electron microscopy revealed heavy damage to the cells and vessels in the treated tumour. Conclusion: PDT with ALA is an effective treatment modality for rat liver tumours.},
  author       = {Liu, D. L and Wang, I and Andersson-Engels, Stefan and Hakansson, C. H and Stenram, U and Svanberg, Katarina},
  issn         = {1473-5687},
  language     = {eng},
  number       = {11},
  pages        = {1073--1080},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {European Journal of Gastroenterology and Hepathology},
  title        = {Intra-operative laser-induced photodynamic therapy in the treatment of experimental hepatic tumours},
  volume       = {7},
  year         = {1995},
}