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Real-time Fluorescence Imaging of Capillary Electrophoresis

Nilsson, S; Johansson, Jonas LU ; Mecklenburg, M; Birnbaum, S; Svanberg, Sune LU ; Wahlund, K. G; Mosbach, K; Miyabayashi, A and Larsson, P. O (1995) In Journal of Capillary Electrophoresis 2(1). p.46-52
Abstract
An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples.... (More)
An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples. The imaging principle was also illustrated with a separation of a mixture of DNA fragments on a commercially available capillary designated for DNA separations. The benefits of real-time imaging for separation processes, in particular for capillary electrophoresis, are discussed. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Capillary Electrophoresis
volume
2
issue
1
pages
46 - 52
publisher
International Scientific Communications Inc.
ISSN
1079-5383
language
English
LU publication?
yes
id
b98797fc-bc4a-4878-9f45-7674afc3ef97 (old id 2259079)
date added to LUP
2012-02-27 21:41:30
date last changed
2016-04-16 06:35:08
@article{b98797fc-bc4a-4878-9f45-7674afc3ef97,
  abstract     = {An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples. The imaging principle was also illustrated with a separation of a mixture of DNA fragments on a commercially available capillary designated for DNA separations. The benefits of real-time imaging for separation processes, in particular for capillary electrophoresis, are discussed.},
  author       = {Nilsson, S and Johansson, Jonas and Mecklenburg, M and Birnbaum, S and Svanberg, Sune and Wahlund, K. G and Mosbach, K and Miyabayashi, A and Larsson, P. O},
  issn         = {1079-5383},
  language     = {eng},
  number       = {1},
  pages        = {46--52},
  publisher    = {International Scientific Communications Inc.},
  series       = {Journal of Capillary Electrophoresis},
  title        = {Real-time Fluorescence Imaging of Capillary Electrophoresis},
  volume       = {2},
  year         = {1995},
}