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Quantifying the absorption and reduced scattering coefficients of tissuelike turbid media over a broad spectral range with noncontact Fourier-transform hyperspectral imaging

Pham, T. H; Bevilacqua, F; Spott, T; Dam, J. S; Tromberg, B. J and Andersson-Engels, Stefan LU (2000) In Applied Optics 39(34). p.6487-6497
Abstract
Absorption (mu (a)) and reduced scattering (mu (s)') spectra of turbid media were quanti fied with a noncontact imaging approach based on a Fourier-transform interferometric imaging system (FTIIS). The FTIIS was used to collect hyperspectral images of the steady-state diffuse reflectance from turbid media. Spatially resolved reflectance data from Monte Carlo simulations were fitted to the recorded hyperspectral images to quantify mu (a) and mu (s)' spectra in the 550-850-nm region. A simple and effective calibration approach was introduced to account for the instrument response. With reflectance data that were close to and far from the source (0.5-6.5 mm), mu (a) and mu (')(s) of homogeneous, semi-infinite turbid phantoms with optical... (More)
Absorption (mu (a)) and reduced scattering (mu (s)') spectra of turbid media were quanti fied with a noncontact imaging approach based on a Fourier-transform interferometric imaging system (FTIIS). The FTIIS was used to collect hyperspectral images of the steady-state diffuse reflectance from turbid media. Spatially resolved reflectance data from Monte Carlo simulations were fitted to the recorded hyperspectral images to quantify mu (a) and mu (s)' spectra in the 550-850-nm region. A simple and effective calibration approach was introduced to account for the instrument response. With reflectance data that were close to and far from the source (0.5-6.5 mm), mu (a) and mu (')(s) of homogeneous, semi-infinite turbid phantoms with optical property ranges comparable with those of tissues were determined with an accuracy of +/-7% and +/-3%, respectively. Prediction accuracy for mu (a) and mu (s)' degraded to +/-12% and +/-4%, respectively, when only reflectance data close to the source (0.5-2.5 mm) were used. Results indicate that reflectance data close to and far hom the source are necessary for optimal quantification of mu (a) and mu (s)'. The spectral properties of mu (a) and mu (s)' values were used to determine the concentrations of absorbers and scatterers, respectively. Absorber and scatterer concentrations of two-chromophore turbid media were determined with an accuracy of +/-5% and +/-3%, respectively. (C) 2000 Optical Society of America OCIS codes: 170.0110, 170.7050, 170.6510, 070.2590, 120.3180. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Optics
volume
39
issue
34
pages
6487 - 6497
publisher
OSA
external identifiers
  • scopus:0001338996
ISSN
2155-3165
DOI
10.1364/AO.39.006487
language
English
LU publication?
yes
id
6e3abcf7-5e39-4346-916c-8e0da20a6cab (old id 2259154)
date added to LUP
2012-02-17 22:28:15
date last changed
2017-10-01 04:58:09
@article{6e3abcf7-5e39-4346-916c-8e0da20a6cab,
  abstract     = {Absorption (mu (a)) and reduced scattering (mu (s)') spectra of turbid media were quanti fied with a noncontact imaging approach based on a Fourier-transform interferometric imaging system (FTIIS). The FTIIS was used to collect hyperspectral images of the steady-state diffuse reflectance from turbid media. Spatially resolved reflectance data from Monte Carlo simulations were fitted to the recorded hyperspectral images to quantify mu (a) and mu (s)' spectra in the 550-850-nm region. A simple and effective calibration approach was introduced to account for the instrument response. With reflectance data that were close to and far from the source (0.5-6.5 mm), mu (a) and mu (')(s) of homogeneous, semi-infinite turbid phantoms with optical property ranges comparable with those of tissues were determined with an accuracy of +/-7% and +/-3%, respectively. Prediction accuracy for mu (a) and mu (s)' degraded to +/-12% and +/-4%, respectively, when only reflectance data close to the source (0.5-2.5 mm) were used. Results indicate that reflectance data close to and far hom the source are necessary for optimal quantification of mu (a) and mu (s)'. The spectral properties of mu (a) and mu (s)' values were used to determine the concentrations of absorbers and scatterers, respectively. Absorber and scatterer concentrations of two-chromophore turbid media were determined with an accuracy of +/-5% and +/-3%, respectively. (C) 2000 Optical Society of America OCIS codes: 170.0110, 170.7050, 170.6510, 070.2590, 120.3180.},
  author       = {Pham, T. H and Bevilacqua, F and Spott, T and Dam, J. S and Tromberg, B. J and Andersson-Engels, Stefan},
  issn         = {2155-3165},
  language     = {eng},
  number       = {34},
  pages        = {6487--6497},
  publisher    = {OSA},
  series       = {Applied Optics},
  title        = {Quantifying the absorption and reduced scattering coefficients of tissuelike turbid media over a broad spectral range with noncontact Fourier-transform hyperspectral imaging},
  url          = {http://dx.doi.org/10.1364/AO.39.006487},
  volume       = {39},
  year         = {2000},
}