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Photobleaching behavior of protoporphyrin IX during 5-aminolevulinic acid marked glioblastoma detection

Haj-Hosseini, N; Richter, J; Andersson-Engels, Stefan LU and Wardell, K (2009) Conference on Photonic Therapeutics and Diagnostics V, San Jose, CA, JAN 24-26, 2009 In PHOTONIC THERAPEUTICS AND DIAGNOSTICS V 7161.
Abstract
The highly malignant brain tumor, glioblastoma multiforme (GBM), is difficult to fully delineate during surgical resection due to its infiltrative ingrowth and morphological similarities to surrounding functioning brain tissue. Selectiveness of GBM to 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX (PpIX) is reported by other researchers to visualize tumor margins under blue light microscopy. To allow objective detection of GBM, a compact and portable fiber optic based fluorescence spectroscopy system is developed. This system is able to deliver excitation laser light (405 nm) in both the continuous and pulsed mode. PpIX fluorescence peaks are detected at 635 and 704 nm, using a fiber-coupled spectrometer. It is necessary to... (More)
The highly malignant brain tumor, glioblastoma multiforme (GBM), is difficult to fully delineate during surgical resection due to its infiltrative ingrowth and morphological similarities to surrounding functioning brain tissue. Selectiveness of GBM to 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX (PpIX) is reported by other researchers to visualize tumor margins under blue light microscopy. To allow objective detection of GBM, a compact and portable fiber optic based fluorescence spectroscopy system is developed. This system is able to deliver excitation laser light (405 nm) in both the continuous and pulsed mode. PpIX fluorescence peaks are detected at 635 and 704 nm, using a fiber-coupled spectrometer. It is necessary to optimize the detection efficiency of the system as the PpIX quickly photobleaches during the laser illumination. A light dose of 2.5 mJ (fluence rate = 9 mJ/mm(2)) is experimentally approved to excite an acceptable level of fluourescence signal arising from glioblastoma. In pulsed illumination mode, an excitation dose of 2.5 mJ, with a dark interval of 0.5 s (duty cycle 50\%) shows a significantly shorter photobleaching time in comparison to the continuous illumination mode with the same laser power (p < 0.05). To avoid photobleaching (the remaining signal is more than 90\% of its initial value) when measuring with 2.5 mJ delivered energy, the time for continuous and pulsed illumination should be restricted to 2.5 and 1.1 s, respectively. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
PHOTONIC THERAPEUTICS AND DIAGNOSTICS V
editor
Kollias, N; Choi, B; Zeng, H; Malek, RS; Wong, BJF; Ilgner, JFR; Gregory, KW; Tearney, GJ; Marcu, L; Hirschberg, H; Madsen, SJ; ; ; ; ; ; ; ; ; ; and
volume
7161
publisher
SPIE
conference name
Conference on Photonic Therapeutics and Diagnostics V, San Jose, CA, JAN 24-26, 2009
external identifiers
  • scopus:66249124276
ISSN
0277-786X
1996-756X
ISBN
978-0-8194-7407-0
DOI
10.1117/12.808156
language
English
LU publication?
yes
id
1be5f339-2c5b-474e-9835-189af00bb8b4 (old id 2260288)
date added to LUP
2012-01-26 23:35:44
date last changed
2017-11-20 15:06:16
@inproceedings{1be5f339-2c5b-474e-9835-189af00bb8b4,
  abstract     = {The highly malignant brain tumor, glioblastoma multiforme (GBM), is difficult to fully delineate during surgical resection due to its infiltrative ingrowth and morphological similarities to surrounding functioning brain tissue. Selectiveness of GBM to 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX (PpIX) is reported by other researchers to visualize tumor margins under blue light microscopy. To allow objective detection of GBM, a compact and portable fiber optic based fluorescence spectroscopy system is developed. This system is able to deliver excitation laser light (405 nm) in both the continuous and pulsed mode. PpIX fluorescence peaks are detected at 635 and 704 nm, using a fiber-coupled spectrometer. It is necessary to optimize the detection efficiency of the system as the PpIX quickly photobleaches during the laser illumination. A light dose of 2.5 mJ (fluence rate = 9 mJ/mm(2)) is experimentally approved to excite an acceptable level of fluourescence signal arising from glioblastoma. In pulsed illumination mode, an excitation dose of 2.5 mJ, with a dark interval of 0.5 s (duty cycle 50\%) shows a significantly shorter photobleaching time in comparison to the continuous illumination mode with the same laser power (p &lt; 0.05). To avoid photobleaching (the remaining signal is more than 90\% of its initial value) when measuring with 2.5 mJ delivered energy, the time for continuous and pulsed illumination should be restricted to 2.5 and 1.1 s, respectively.},
  author       = {Haj-Hosseini, N and Richter, J and Andersson-Engels, Stefan and Wardell, K},
  booktitle    = {PHOTONIC THERAPEUTICS AND DIAGNOSTICS V},
  editor       = {Kollias, N and Choi, B and Zeng, H and Malek, RS and Wong, BJF and Ilgner, JFR and Gregory, KW and Tearney, GJ and Marcu, L and Hirschberg, H and Madsen, SJ},
  isbn         = {978-0-8194-7407-0},
  issn         = {0277-786X},
  language     = {eng},
  publisher    = {SPIE},
  title        = {Photobleaching behavior of protoporphyrin IX during 5-aminolevulinic acid marked glioblastoma detection},
  url          = {http://dx.doi.org/10.1117/12.808156},
  volume       = {7161},
  year         = {2009},
}