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Laser-induced fluorescence diagnostics of basal cell carcinomas of the skin following topical ALA application

af Klinteberg, C; Nilsson, AMK; Wang, I; Andersson-Engels, Stefan LU ; Svanberg, Sune LU and Svanberg, Katarina LU (1996) Conference on Optical Biopsies and Microscopic Techniques, VIENNA, AUSTRIA, SEP 07-09, 1996 In Optical Biopsies and Microscopic Techniques, Proceedings of 2926. p.32-40
Abstract
Fourteen patients with superficial basal cell carcinomas (BCCs) and fifteen patients with nodular BCCs were investigated by means of laser-induced fluorescence (LIF) in connection with photodynamic therapy (PDT). Topical application of delta-amino levulinic acid (ALA) was performed six hours prior to the treatment session. Fluorescence spectra were recorded, using a point-monitoring system with an excitation wavelength of 405 nm. The measurements were performed in scans over the lesion and the surrounding normal skin before application of ALA, and immediately before and after the laser treatment. The selective uptake of the photosensitiser resulted in a fluorescence intensity ratio of 2.4:1 for superficial BCCs and 2.5:1 for nodular BCCs.... (More)
Fourteen patients with superficial basal cell carcinomas (BCCs) and fifteen patients with nodular BCCs were investigated by means of laser-induced fluorescence (LIF) in connection with photodynamic therapy (PDT). Topical application of delta-amino levulinic acid (ALA) was performed six hours prior to the treatment session. Fluorescence spectra were recorded, using a point-monitoring system with an excitation wavelength of 405 nm. The measurements were performed in scans over the lesion and the surrounding normal skin before application of ALA, and immediately before and after the laser treatment. The selective uptake of the photosensitiser resulted in a fluorescence intensity ratio of 2.4:1 for superficial BCCs and 2.5:1 for nodular BCCs. If the fluorescence intensity was divided by the autofluorescence, this resulted in a contrast enhancement of about a factor 6 for tumour tissue. In seven patients (five with nodular BCC and two with superficial BCC), additional fluorescence measurements were performed two and four hours following the ALA application, and two hours after the PDT procedure. Thus, the kinetics of the transformation of ACA to protoporphyrin IX (PpIX) could be followed, which indicated that the synthesis of PpIX was more rapid in the tumour than in the normal tissue. After four hours, the PpIX level inside the tumour was saturated, while there still was an accumulation in the surrounding skin. The highest contrast between tumour and normal skin was reached within two hours after the ALA application. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
Optical Biopsies and Microscopic Techniques, Proceedings of
editor
Bigio, IJ; Grundfest, WS; Schneckenburger, H; Svanberg, Katarina; Viallet, PM; Katzir, A; ; ; ; ; and
volume
2926
pages
32 - 40
publisher
The Society of Photo-optical Instrumentation Engineers (SPIE)
conference name
Conference on Optical Biopsies and Microscopic Techniques, VIENNA, AUSTRIA, SEP 07-09, 1996
external identifiers
  • scopus:0030354479
ISBN
0-8194-2328-9
DOI
10.1117/12.260817
language
English
LU publication?
yes
id
1a902804-8961-4ae8-825a-871a76cab61b (old id 2260477)
date added to LUP
2012-01-26 23:31:02
date last changed
2017-01-01 08:08:36
@inproceedings{1a902804-8961-4ae8-825a-871a76cab61b,
  abstract     = {Fourteen patients with superficial basal cell carcinomas (BCCs) and fifteen patients with nodular BCCs were investigated by means of laser-induced fluorescence (LIF) in connection with photodynamic therapy (PDT). Topical application of delta-amino levulinic acid (ALA) was performed six hours prior to the treatment session. Fluorescence spectra were recorded, using a point-monitoring system with an excitation wavelength of 405 nm. The measurements were performed in scans over the lesion and the surrounding normal skin before application of ALA, and immediately before and after the laser treatment. The selective uptake of the photosensitiser resulted in a fluorescence intensity ratio of 2.4:1 for superficial BCCs and 2.5:1 for nodular BCCs. If the fluorescence intensity was divided by the autofluorescence, this resulted in a contrast enhancement of about a factor 6 for tumour tissue. In seven patients (five with nodular BCC and two with superficial BCC), additional fluorescence measurements were performed two and four hours following the ALA application, and two hours after the PDT procedure. Thus, the kinetics of the transformation of ACA to protoporphyrin IX (PpIX) could be followed, which indicated that the synthesis of PpIX was more rapid in the tumour than in the normal tissue. After four hours, the PpIX level inside the tumour was saturated, while there still was an accumulation in the surrounding skin. The highest contrast between tumour and normal skin was reached within two hours after the ALA application.},
  author       = {af Klinteberg, C and Nilsson, AMK and Wang, I and Andersson-Engels, Stefan and Svanberg, Sune and Svanberg, Katarina},
  booktitle    = {Optical Biopsies and Microscopic Techniques, Proceedings of},
  editor       = {Bigio, IJ and Grundfest, WS and Schneckenburger, H and Svanberg, Katarina and Viallet, PM and Katzir, A},
  isbn         = {0-8194-2328-9},
  language     = {eng},
  pages        = {32--40},
  publisher    = {The Society of Photo-optical Instrumentation Engineers (SPIE)},
  title        = {Laser-induced fluorescence diagnostics of basal cell carcinomas of the skin following topical ALA application},
  url          = {http://dx.doi.org/10.1117/12.260817},
  volume       = {2926},
  year         = {1996},
}