Advanced

Optical properties of human whole blood - Changes due to slow heating

Nilsson, A. M. K; Lucassen, G. W; Verkmysse, W; Andersson-Engels, Stefan LU and van Gemert, Martin .j. C. (1996) Conference on Laser-Tissue Interaction and Tissue Optics II, VIENNA, AUSTRIA, SEP 07-10, 1996 In LASER-TISSUE INTERACTION AND TISSUE OPTICS II, PROCEEDINGS OF 2923. p.24-34
Abstract
Optical properties of human whole blood were measured in vitro, at 633 nm with a double integrating sphere set-up, The blood was kept at constant flow through a flow cell while slowly heating the blood from approximately 25 degrees C to 55 degrees C in a heat exchanger, The results show a small but distinct decrease in the g-factor of 1.7 +/- 0.6\% and a similar increase in the scattering coefficient, mu(s), of 2.9 +/- 0.6\% at approximately 45-46 degrees C. When studying the thermal effect on the blood cells under a white-light transmission microscope, the changes in the scattering properties could be correlated to a sudden change in the shape of the red blood cells, from disc-shaped to spherical, at approximately the same temperature.... (More)
Optical properties of human whole blood were measured in vitro, at 633 nm with a double integrating sphere set-up, The blood was kept at constant flow through a flow cell while slowly heating the blood from approximately 25 degrees C to 55 degrees C in a heat exchanger, The results show a small but distinct decrease in the g-factor of 1.7 +/- 0.6\% and a similar increase in the scattering coefficient, mu(s), of 2.9 +/- 0.6\% at approximately 45-46 degrees C. When studying the thermal effect on the blood cells under a white-light transmission microscope, the changes in the scattering properties could be correlated to a sudden change in the shape of the red blood cells, from disc-shaped to spherical, at approximately the same temperature. Furthermore, a continuous manifest increase in the absorption coefficient, mu(a), was seen with temperature rise, on average 83.8 +/- 68.1\% when reaching the temperature 50 degrees C. This might be due to heat-induced haemolysis of the red blood cells, resulting in free light absorbing haemoglobin in the surrounding plasma and thus higher effective light absorption. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
LASER-TISSUE INTERACTION AND TISSUE OPTICS II, PROCEEDINGS OF
editor
Albrecht, HJ; Delacretaz, GP; Meier, TH; Steiner, RW; Svaasand, LO and Katzir, A
volume
2923
pages
24 - 34
publisher
The Society of Photo-optical Instrumentation Engineers (SPIE)
conference name
Conference on Laser-Tissue Interaction and Tissue Optics II, VIENNA, AUSTRIA, SEP 07-10, 1996
external identifiers
  • Scopus:0030368104
ISBN
0-8194-2325-4
DOI
10.1117/12.260746
language
English
LU publication?
yes
id
ed51f4b0-8e52-47e6-9606-35a6e8c59470 (old id 2260675)
date added to LUP
2012-01-26 23:28:02
date last changed
2017-01-01 08:01:06
@inproceedings{ed51f4b0-8e52-47e6-9606-35a6e8c59470,
  abstract     = {Optical properties of human whole blood were measured in vitro, at 633 nm with a double integrating sphere set-up, The blood was kept at constant flow through a flow cell while slowly heating the blood from approximately 25 degrees C to 55 degrees C in a heat exchanger, The results show a small but distinct decrease in the g-factor of 1.7 +/- 0.6\% and a similar increase in the scattering coefficient, mu(s), of 2.9 +/- 0.6\% at approximately 45-46 degrees C. When studying the thermal effect on the blood cells under a white-light transmission microscope, the changes in the scattering properties could be correlated to a sudden change in the shape of the red blood cells, from disc-shaped to spherical, at approximately the same temperature. Furthermore, a continuous manifest increase in the absorption coefficient, mu(a), was seen with temperature rise, on average 83.8 +/- 68.1\% when reaching the temperature 50 degrees C. This might be due to heat-induced haemolysis of the red blood cells, resulting in free light absorbing haemoglobin in the surrounding plasma and thus higher effective light absorption.},
  author       = {Nilsson, A. M. K and Lucassen, G. W and Verkmysse, W and Andersson-Engels, Stefan and van Gemert, Martin .j. C.},
  booktitle    = {LASER-TISSUE INTERACTION AND TISSUE OPTICS II, PROCEEDINGS OF},
  editor       = {Albrecht, HJ and Delacretaz, GP and Meier, TH and Steiner, RW and Svaasand, LO and Katzir, A},
  isbn         = {0-8194-2325-4},
  language     = {eng},
  pages        = {24--34},
  publisher    = {The Society of Photo-optical Instrumentation Engineers (SPIE)},
  title        = {Optical properties of human whole blood - Changes due to slow heating},
  url          = {http://dx.doi.org/10.1117/12.260746},
  volume       = {2923},
  year         = {1996},
}