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MRNA expression and localization of bNOS, eNOS and iNOS in human cervix at preterm and term labour

Tornblom, SA; Maul, H; Klimaviciute, A; Garfield, RE; Bystrom, B; Malmström, Anders LU and Ekman-Ordeberg, G (2005) In Reproductive Biology and Endocrinology 3(33).
Abstract
Background: Preterm birth is the primary cause of the neonatal mortality and morbidity. There will be no preterm birth without a cervical softening. Nitric oxide ( NO) is shown to be a mediator of term cervical ripening. The aim of this study was to investigate mRNA expression of the three isomers of NO synthases ( NOS) and to identify them by immunohistochemistry in the human cervix at preterm birth compared to term. Methods: The three isomers of NOS- inducible ( iNOS), endothelial ( eNOS) and neuronal ( bNOS) - were investigated in the human cervix. The expression of mRNA was determined using RealTime Multiplex RT- PCR. The localisation of synthases in the cervical tissue was analysed using immunohistochemistry. Cervical biopsies were... (More)
Background: Preterm birth is the primary cause of the neonatal mortality and morbidity. There will be no preterm birth without a cervical softening. Nitric oxide ( NO) is shown to be a mediator of term cervical ripening. The aim of this study was to investigate mRNA expression of the three isomers of NO synthases ( NOS) and to identify them by immunohistochemistry in the human cervix at preterm birth compared to term. Methods: The three isomers of NOS- inducible ( iNOS), endothelial ( eNOS) and neuronal ( bNOS) - were investigated in the human cervix. The expression of mRNA was determined using RealTime Multiplex RT- PCR. The localisation of synthases in the cervical tissue was analysed using immunohistochemistry. Cervical biopsies were obtained from 4 groups of women without clinical signs of infection: preterm ( PTL), term labour ( TL), preterm not in labour ( PTnotL) and term not in labour ( TnotL) patients. One- Way ANOVA, Kruskal- Wallis, Student t- test or Mann- Whitney test were applied as appropriate to determine statistically significant differences among the groups. Results: Patients in preterm labour had significantly ( p < 0.01) higher mRNA levels of all the three NOS isomers compared to those in term labour. Women not in labour, irrespective of gestational age, thus with unripe cervices, had significantly lower eNOS mRNA levels compared to those in labour ( p < 0.01). Immunoreactivity for all three NO synthases was observed in each examined sample in all groups. The bNOS staining was the most prominent. Conclusion: The mRNA levels were higher in the preterm labour group compared to the women at term labour. The significant increase of the eNOS mRNA expression, from the unripe to the favourable cervical state during labour, may indicate a role of eNOS and supports the role of NO in the cervical ripening process. All the three synthases were identified by immunohistochemistry in all the groups of study. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Reproductive Biology and Endocrinology
volume
3
issue
33
publisher
BioMed Central
external identifiers
  • wos:000231363500001
  • scopus:27344452569
ISSN
1477-7827
DOI
10.1186/1477-7827-3-33
language
English
LU publication?
yes
id
10c13adb-3d8a-40b4-afd8-53fdc1f2d74d (old id 226538)
date added to LUP
2007-08-20 10:21:07
date last changed
2017-10-29 04:19:48
@article{10c13adb-3d8a-40b4-afd8-53fdc1f2d74d,
  abstract     = {Background: Preterm birth is the primary cause of the neonatal mortality and morbidity. There will be no preterm birth without a cervical softening. Nitric oxide ( NO) is shown to be a mediator of term cervical ripening. The aim of this study was to investigate mRNA expression of the three isomers of NO synthases ( NOS) and to identify them by immunohistochemistry in the human cervix at preterm birth compared to term. Methods: The three isomers of NOS- inducible ( iNOS), endothelial ( eNOS) and neuronal ( bNOS) - were investigated in the human cervix. The expression of mRNA was determined using RealTime Multiplex RT- PCR. The localisation of synthases in the cervical tissue was analysed using immunohistochemistry. Cervical biopsies were obtained from 4 groups of women without clinical signs of infection: preterm ( PTL), term labour ( TL), preterm not in labour ( PTnotL) and term not in labour ( TnotL) patients. One- Way ANOVA, Kruskal- Wallis, Student t- test or Mann- Whitney test were applied as appropriate to determine statistically significant differences among the groups. Results: Patients in preterm labour had significantly ( p &lt; 0.01) higher mRNA levels of all the three NOS isomers compared to those in term labour. Women not in labour, irrespective of gestational age, thus with unripe cervices, had significantly lower eNOS mRNA levels compared to those in labour ( p &lt; 0.01). Immunoreactivity for all three NO synthases was observed in each examined sample in all groups. The bNOS staining was the most prominent. Conclusion: The mRNA levels were higher in the preterm labour group compared to the women at term labour. The significant increase of the eNOS mRNA expression, from the unripe to the favourable cervical state during labour, may indicate a role of eNOS and supports the role of NO in the cervical ripening process. All the three synthases were identified by immunohistochemistry in all the groups of study.},
  author       = {Tornblom, SA and Maul, H and Klimaviciute, A and Garfield, RE and Bystrom, B and Malmström, Anders and Ekman-Ordeberg, G},
  issn         = {1477-7827},
  language     = {eng},
  number       = {33},
  publisher    = {BioMed Central},
  series       = {Reproductive Biology and Endocrinology},
  title        = {MRNA expression and localization of bNOS, eNOS and iNOS in human cervix at preterm and term labour},
  url          = {http://dx.doi.org/10.1186/1477-7827-3-33},
  volume       = {3},
  year         = {2005},
}