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The YjbH adaptor protein enhances proteolysis of the transcriptional regulator Spx in Staphylococcus aureus.

Engman, Jakob LU ; Rogstam, Annika LU ; Frees, Dorte; Ingmer, Hanne and von Wachenfeldt, Claes LU (2012) In Journal of Bacteriology 194(5). p.1186-1194
Abstract
Spx is a global regulator that is widespread among the low G+C Gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under non-stress conditions, Spx is rapidly degraded by the ClpXP protease. This degradation is enhanced by the YjbH adaptor protein. Upon disulfide stress, the amount of Spx rapidly increases due to a decrease in degradation. In the opportunistic pathogen Staphylococcus aureus, Spx is a global regulator influencing growth, biofilm formation and general stress protection, and cells lacking the spx gene exhibit poor growth also under non-stress conditions. To investigate the mechanism by which the activity... (More)
Spx is a global regulator that is widespread among the low G+C Gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under non-stress conditions, Spx is rapidly degraded by the ClpXP protease. This degradation is enhanced by the YjbH adaptor protein. Upon disulfide stress, the amount of Spx rapidly increases due to a decrease in degradation. In the opportunistic pathogen Staphylococcus aureus, Spx is a global regulator influencing growth, biofilm formation and general stress protection, and cells lacking the spx gene exhibit poor growth also under non-stress conditions. To investigate the mechanism by which the activity of Spx is regulated we identified a homolog in S. aureus of the B. subtilis yjbH gene. The gene encodes a protein that shows approximately 30% sequence identity to YjbH of B. subtilis. Heterologous expression of S. aureus yjbH in a B. subtilis yjbH mutant restored Spx to wild type levels both under non-stress conditions and under conditions of disulfide stress. From these studies we conclude that the two YjbH homologues have a conserved physiological function. Accordingly, inactivation of yjbH in S. aureus increased the level of Spx protein and transcription of the Spx-regulated gene trxB. Notably, the yjbH mutant exhibited reduced growth and increased pigmentation, and both phenotypes were reversed by complementation of the yjbH gene. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
194
issue
5
pages
1186 - 1194
publisher
American Society for Microbiology
external identifiers
  • wos:000300530800030
  • pmid:22194450
  • scopus:84857871745
ISSN
0021-9193
DOI
10.1128/JB.06414-11
language
English
LU publication?
yes
id
1c3eaed5-301d-4c04-a214-ddbb2b169239 (old id 2273590)
date added to LUP
2012-02-06 13:37:44
date last changed
2017-10-01 03:23:54
@article{1c3eaed5-301d-4c04-a214-ddbb2b169239,
  abstract     = {Spx is a global regulator that is widespread among the low G+C Gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under non-stress conditions, Spx is rapidly degraded by the ClpXP protease. This degradation is enhanced by the YjbH adaptor protein. Upon disulfide stress, the amount of Spx rapidly increases due to a decrease in degradation. In the opportunistic pathogen Staphylococcus aureus, Spx is a global regulator influencing growth, biofilm formation and general stress protection, and cells lacking the spx gene exhibit poor growth also under non-stress conditions. To investigate the mechanism by which the activity of Spx is regulated we identified a homolog in S. aureus of the B. subtilis yjbH gene. The gene encodes a protein that shows approximately 30% sequence identity to YjbH of B. subtilis. Heterologous expression of S. aureus yjbH in a B. subtilis yjbH mutant restored Spx to wild type levels both under non-stress conditions and under conditions of disulfide stress. From these studies we conclude that the two YjbH homologues have a conserved physiological function. Accordingly, inactivation of yjbH in S. aureus increased the level of Spx protein and transcription of the Spx-regulated gene trxB. Notably, the yjbH mutant exhibited reduced growth and increased pigmentation, and both phenotypes were reversed by complementation of the yjbH gene.},
  author       = {Engman, Jakob and Rogstam, Annika and Frees, Dorte and Ingmer, Hanne and von Wachenfeldt, Claes},
  issn         = {0021-9193},
  language     = {eng},
  number       = {5},
  pages        = {1186--1194},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {The YjbH adaptor protein enhances proteolysis of the transcriptional regulator Spx in Staphylococcus aureus.},
  url          = {http://dx.doi.org/10.1128/JB.06414-11},
  volume       = {194},
  year         = {2012},
}