Autocrine epidermal growth factor receptor ligand production and cetuximab response in head and neck squamous cell carcinoma cell lines.
(2012) In Journal of Cancer Research and Clinical Oncology 138(3). p.491-499- Abstract
- PURPOSE: Predictive strategies for the treatment efficacy of cetuximab are currently not available for head and neck cancer. We investigated the correlation between the expression of epidermal growth factor receptor (EGFR) ligands and EGFR expression, and the growth inhibitory activity of cetuximab in a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. METHODS: The growth inhibiting effect of cetuximab was measured for eight HNSCC cell lines and correlated with the autocrine production of five EGFR ligands as measured by ELISA, and the mRNA expression of two ligands, as measured by quantitative RT-PCR. EGFR expression was assessed by western blot analysis. RESULTS: There was a good correlation between the expression of... (More)
- PURPOSE: Predictive strategies for the treatment efficacy of cetuximab are currently not available for head and neck cancer. We investigated the correlation between the expression of epidermal growth factor receptor (EGFR) ligands and EGFR expression, and the growth inhibitory activity of cetuximab in a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. METHODS: The growth inhibiting effect of cetuximab was measured for eight HNSCC cell lines and correlated with the autocrine production of five EGFR ligands as measured by ELISA, and the mRNA expression of two ligands, as measured by quantitative RT-PCR. EGFR expression was assessed by western blot analysis. RESULTS: There was a good correlation between the expression of four of the EGFR ligands (TGF-α, amphiregulin, epiregulin and epigen) and the growth inhibiting effect of cetuximab. TGF-α had the highest predictive potential but had to be combined with epigen for full prediction. EGFR expression also correlated with cetuximab sensitivity but less clearly. CONCLUSIONS: The results indicate that the expression of several EGFR ligands has to be used to predict sensitivity to cetuximab in HNSCC. This has to be further evaluated in clinical samples. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2273609
- author
- Oshima, Goro ; Wennerberg, Johan LU ; Yamatodani, Takashi ; Kjellén, Elisabeth LU ; Mineta, Hiroyuki ; Johnsson, Anders LU and Ekblad, Lars LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Cancer Research and Clinical Oncology
- volume
- 138
- issue
- 3
- pages
- 491 - 499
- publisher
- Springer
- external identifiers
-
- wos:000300345000015
- pmid:22193422
- scopus:84859794327
- ISSN
- 1432-1335
- DOI
- 10.1007/s00432-011-1127-5
- language
- English
- LU publication?
- yes
- id
- f97a54f6-d0b1-4fda-9e2a-d7ccfe7a0b73 (old id 2273609)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22193422?dopt=Abstract
- date added to LUP
- 2016-04-04 08:34:41
- date last changed
- 2022-04-23 17:43:07
@article{f97a54f6-d0b1-4fda-9e2a-d7ccfe7a0b73, abstract = {{PURPOSE: Predictive strategies for the treatment efficacy of cetuximab are currently not available for head and neck cancer. We investigated the correlation between the expression of epidermal growth factor receptor (EGFR) ligands and EGFR expression, and the growth inhibitory activity of cetuximab in a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. METHODS: The growth inhibiting effect of cetuximab was measured for eight HNSCC cell lines and correlated with the autocrine production of five EGFR ligands as measured by ELISA, and the mRNA expression of two ligands, as measured by quantitative RT-PCR. EGFR expression was assessed by western blot analysis. RESULTS: There was a good correlation between the expression of four of the EGFR ligands (TGF-α, amphiregulin, epiregulin and epigen) and the growth inhibiting effect of cetuximab. TGF-α had the highest predictive potential but had to be combined with epigen for full prediction. EGFR expression also correlated with cetuximab sensitivity but less clearly. CONCLUSIONS: The results indicate that the expression of several EGFR ligands has to be used to predict sensitivity to cetuximab in HNSCC. This has to be further evaluated in clinical samples.}}, author = {{Oshima, Goro and Wennerberg, Johan and Yamatodani, Takashi and Kjellén, Elisabeth and Mineta, Hiroyuki and Johnsson, Anders and Ekblad, Lars}}, issn = {{1432-1335}}, language = {{eng}}, number = {{3}}, pages = {{491--499}}, publisher = {{Springer}}, series = {{Journal of Cancer Research and Clinical Oncology}}, title = {{Autocrine epidermal growth factor receptor ligand production and cetuximab response in head and neck squamous cell carcinoma cell lines.}}, url = {{http://dx.doi.org/10.1007/s00432-011-1127-5}}, doi = {{10.1007/s00432-011-1127-5}}, volume = {{138}}, year = {{2012}}, }