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Autocrine epidermal growth factor receptor ligand production and cetuximab response in head and neck squamous cell carcinoma cell lines.

Oshima, Goro; Wennerberg, Johan LU ; Yamatodani, Takashi; Kjellén, Elisabeth LU ; Mineta, Hiroyuki; Johnsson, Anders LU and Ekblad, Lars LU (2012) In Journal of Cancer Research and Clinical Oncology 138(3). p.491-499
Abstract
PURPOSE: Predictive strategies for the treatment efficacy of cetuximab are currently not available for head and neck cancer. We investigated the correlation between the expression of epidermal growth factor receptor (EGFR) ligands and EGFR expression, and the growth inhibitory activity of cetuximab in a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. METHODS: The growth inhibiting effect of cetuximab was measured for eight HNSCC cell lines and correlated with the autocrine production of five EGFR ligands as measured by ELISA, and the mRNA expression of two ligands, as measured by quantitative RT-PCR. EGFR expression was assessed by western blot analysis. RESULTS: There was a good correlation between the expression of... (More)
PURPOSE: Predictive strategies for the treatment efficacy of cetuximab are currently not available for head and neck cancer. We investigated the correlation between the expression of epidermal growth factor receptor (EGFR) ligands and EGFR expression, and the growth inhibitory activity of cetuximab in a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. METHODS: The growth inhibiting effect of cetuximab was measured for eight HNSCC cell lines and correlated with the autocrine production of five EGFR ligands as measured by ELISA, and the mRNA expression of two ligands, as measured by quantitative RT-PCR. EGFR expression was assessed by western blot analysis. RESULTS: There was a good correlation between the expression of four of the EGFR ligands (TGF-α, amphiregulin, epiregulin and epigen) and the growth inhibiting effect of cetuximab. TGF-α had the highest predictive potential but had to be combined with epigen for full prediction. EGFR expression also correlated with cetuximab sensitivity but less clearly. CONCLUSIONS: The results indicate that the expression of several EGFR ligands has to be used to predict sensitivity to cetuximab in HNSCC. This has to be further evaluated in clinical samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Cancer Research and Clinical Oncology
volume
138
issue
3
pages
491 - 499
publisher
Springer
external identifiers
  • wos:000300345000015
  • pmid:22193422
  • scopus:84859794327
ISSN
1432-1335
DOI
10.1007/s00432-011-1127-5
language
English
LU publication?
yes
id
f97a54f6-d0b1-4fda-9e2a-d7ccfe7a0b73 (old id 2273609)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22193422?dopt=Abstract
date added to LUP
2012-01-03 22:29:17
date last changed
2017-09-24 04:38:50
@article{f97a54f6-d0b1-4fda-9e2a-d7ccfe7a0b73,
  abstract     = {PURPOSE: Predictive strategies for the treatment efficacy of cetuximab are currently not available for head and neck cancer. We investigated the correlation between the expression of epidermal growth factor receptor (EGFR) ligands and EGFR expression, and the growth inhibitory activity of cetuximab in a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. METHODS: The growth inhibiting effect of cetuximab was measured for eight HNSCC cell lines and correlated with the autocrine production of five EGFR ligands as measured by ELISA, and the mRNA expression of two ligands, as measured by quantitative RT-PCR. EGFR expression was assessed by western blot analysis. RESULTS: There was a good correlation between the expression of four of the EGFR ligands (TGF-α, amphiregulin, epiregulin and epigen) and the growth inhibiting effect of cetuximab. TGF-α had the highest predictive potential but had to be combined with epigen for full prediction. EGFR expression also correlated with cetuximab sensitivity but less clearly. CONCLUSIONS: The results indicate that the expression of several EGFR ligands has to be used to predict sensitivity to cetuximab in HNSCC. This has to be further evaluated in clinical samples.},
  author       = {Oshima, Goro and Wennerberg, Johan and Yamatodani, Takashi and Kjellén, Elisabeth and Mineta, Hiroyuki and Johnsson, Anders and Ekblad, Lars},
  issn         = {1432-1335},
  language     = {eng},
  number       = {3},
  pages        = {491--499},
  publisher    = {Springer},
  series       = {Journal of Cancer Research and Clinical Oncology},
  title        = {Autocrine epidermal growth factor receptor ligand production and cetuximab response in head and neck squamous cell carcinoma cell lines.},
  url          = {http://dx.doi.org/10.1007/s00432-011-1127-5},
  volume       = {138},
  year         = {2012},
}