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A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.

Rasmussen, Anna LU ; Lv, Y; Schnackerz, Klaus LU and Piskur, Jure LU (2011) In Nucleosides, Nucleotides & Nucleic Acids 30(12). p.1227-1232
Abstract
We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Expression vector, Saccharomyces (Lachancea) kluyveri, URC pathway, catabolism of pyrimidines
in
Nucleosides, Nucleotides & Nucleic Acids
volume
30
issue
12
pages
1227 - 1232
publisher
Taylor & Francis
external identifiers
  • wos:000298738500031
  • pmid:22132979
  • scopus:84855904015
ISSN
1525-7770
DOI
10.1080/15257770.2011.603713
language
English
LU publication?
yes
id
f2665c9b-a074-4024-8cd6-c51bfbfc8758 (old id 2274697)
date added to LUP
2012-02-07 16:54:29
date last changed
2017-01-01 04:10:13
@article{f2665c9b-a074-4024-8cd6-c51bfbfc8758,
  abstract     = {We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.},
  author       = {Rasmussen, Anna and Lv, Y and Schnackerz, Klaus and Piskur, Jure},
  issn         = {1525-7770},
  keyword      = {Expression vector,Saccharomyces (Lachancea) kluyveri,URC pathway,catabolism of pyrimidines},
  language     = {eng},
  number       = {12},
  pages        = {1227--1232},
  publisher    = {Taylor & Francis},
  series       = {Nucleosides, Nucleotides & Nucleic Acids},
  title        = {A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.},
  url          = {http://dx.doi.org/10.1080/15257770.2011.603713},
  volume       = {30},
  year         = {2011},
}