Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

A simplified approach to prepare controlled cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage

Porras Hernández, Ana María ; Pohlit, Hannah ; Sjögren, Frida ; Shi, L ; Ossipov, D ; Antfolk, Maria LU and Tenje, Maria LU (2020) In Journal of Materials Science: Materials in Medicine 31.
Abstract
In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine–glycine–aspartate onto the hydrogel surface via radical mediated thiol–ene reaction using the non-reacted Am... (More)
In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine–glycine–aspartate onto the hydrogel surface via radical mediated thiol–ene reaction using the non-reacted Am groups. We show that 10 × 10 µm2 squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 × 25 µm2 squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 × 100 µm2 square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Materials Science: Materials in Medicine
volume
31
article number
89
publisher
Springer
ISSN
1573-4838
DOI
10.1007/s10856-020-06446-x
language
English
LU publication?
no
id
2285df29-dfc1-4169-a6fc-2581672e07d4
date added to LUP
2024-04-04 19:20:29
date last changed
2024-04-05 15:04:49
@article{2285df29-dfc1-4169-a6fc-2581672e07d4,
  abstract     = {{In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine–glycine–aspartate onto the hydrogel surface via radical mediated thiol–ene reaction using the non-reacted Am groups. We show that 10 × 10 µm2 squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 × 25 µm2 squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 × 100 µm2 square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology.}},
  author       = {{Porras Hernández, Ana María and Pohlit, Hannah and Sjögren, Frida and Shi, L and Ossipov, D and Antfolk, Maria and Tenje, Maria}},
  issn         = {{1573-4838}},
  language     = {{eng}},
  publisher    = {{Springer}},
  series       = {{Journal of Materials Science: Materials in Medicine}},
  title        = {{A simplified approach to prepare controlled cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage}},
  url          = {{http://dx.doi.org/10.1007/s10856-020-06446-x}},
  doi          = {{10.1007/s10856-020-06446-x}},
  volume       = {{31}},
  year         = {{2020}},
}