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Icosahedral capsid formation by capsomer subunits and a semiflexible polyion

Zhang, Ran LU ; Wernersson, Erik LU and Linse, Per LU (2013) In RSC Advances 3(47). p.25258-25267
Abstract
The role of the genome in the assembly of icosahedral viral capsids has been investigated by molecular dynamics simulation of a coarse-grained model, in which the capsomers carry explicit charges and the polynucleic acid is represented by a bead-spring chain. The co-assembly process was contrasted with the self-assembly of uncharged capsomers. In the co-assembly, the capsomers first associated to the polyion and then rearrange into a capsid, whereas the self-assembly proceeded through a spontaneous nucleation and growth of partial capsids. The polyion backbone stiffness was found to have a significant effect on the co-assembly process; polyions of intermediate flexibility gave the fastest and most faithful assembly process. Addition of a... (More)
The role of the genome in the assembly of icosahedral viral capsids has been investigated by molecular dynamics simulation of a coarse-grained model, in which the capsomers carry explicit charges and the polynucleic acid is represented by a bead-spring chain. The co-assembly process was contrasted with the self-assembly of uncharged capsomers. In the co-assembly, the capsomers first associated to the polyion and then rearrange into a capsid, whereas the self-assembly proceeded through a spontaneous nucleation and growth of partial capsids. The polyion backbone stiffness was found to have a significant effect on the co-assembly process; polyions of intermediate flexibility gave the fastest and most faithful assembly process. Addition of a small amount of monovalent salt also improved both speed and fidelity of the co-assembly process. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
RSC Advances
volume
3
issue
47
pages
25258 - 25267
publisher
Royal Society of Chemistry
external identifiers
  • wos:000327261000066
  • scopus:84887843192
ISSN
2046-2069
DOI
10.1039/c3ra44533j
language
English
LU publication?
yes
id
229efab5-1029-414b-ba66-2b122ae4db48 (old id 4275359)
date added to LUP
2014-01-28 11:02:30
date last changed
2019-02-20 06:32:59
@article{229efab5-1029-414b-ba66-2b122ae4db48,
  abstract     = {The role of the genome in the assembly of icosahedral viral capsids has been investigated by molecular dynamics simulation of a coarse-grained model, in which the capsomers carry explicit charges and the polynucleic acid is represented by a bead-spring chain. The co-assembly process was contrasted with the self-assembly of uncharged capsomers. In the co-assembly, the capsomers first associated to the polyion and then rearrange into a capsid, whereas the self-assembly proceeded through a spontaneous nucleation and growth of partial capsids. The polyion backbone stiffness was found to have a significant effect on the co-assembly process; polyions of intermediate flexibility gave the fastest and most faithful assembly process. Addition of a small amount of monovalent salt also improved both speed and fidelity of the co-assembly process.},
  author       = {Zhang, Ran and Wernersson, Erik and Linse, Per},
  issn         = {2046-2069},
  language     = {eng},
  number       = {47},
  pages        = {25258--25267},
  publisher    = {Royal Society of Chemistry},
  series       = {RSC Advances},
  title        = {Icosahedral capsid formation by capsomer subunits and a semiflexible polyion},
  url          = {http://dx.doi.org/10.1039/c3ra44533j},
  volume       = {3},
  year         = {2013},
}