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Cytotoxicity and apoptotic effects of microcystin-LR and anatoxin-a in mouse lymphocytes

Teneva, I; Mladenov, R; Popov, N and Dzhambazov, Balik LU (2005) In Folia Biologica 51(3). p.62-67
Abstract
There is an increasing amount of knowledge on the cytotoxic properties of cyanotoxins, but relatively little is known regarding their fine specificity and mechanisms of action. In this study, we investigated the influence of microcystin-LR and AnTx-a on mouse B- and T-lymphocyte subpopulations in vitro. Cyanotoxins significantly decreased the cell viability after 4 and 24 h, compared to the untreated control. After 24 h exposure to microcystin-LR and anatoxin-a, the viability of splenocytes dropped to 23% and 57%, respectively. Our data demonstrate that microcystin-LR induced apoptosis specifically in mouse B cells, probably via the B-cell antigen receptor and mitochondrial pathway, while the T cells were not affected. AnTx-a showed... (More)
There is an increasing amount of knowledge on the cytotoxic properties of cyanotoxins, but relatively little is known regarding their fine specificity and mechanisms of action. In this study, we investigated the influence of microcystin-LR and AnTx-a on mouse B- and T-lymphocyte subpopulations in vitro. Cyanotoxins significantly decreased the cell viability after 4 and 24 h, compared to the untreated control. After 24 h exposure to microcystin-LR and anatoxin-a, the viability of splenocytes dropped to 23% and 57%, respectively. Our data demonstrate that microcystin-LR induced apoptosis specifically in mouse B cells, probably via the B-cell antigen receptor and mitochondrial pathway, while the T cells were not affected. AnTx-a showed cytotoxic effects on both lymphocyte subpopulations, but the effects were driven by mechanisms different from apoptosis. These findings demonstrate that the cyanotoxins could cause cytotoxic alterations in a variety of cell types different from the major targets, operating via distinct mechanisms. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cytotoxicity, microcystin-LR, anatoxin-a, mouse lymphocytes, apoptosis
in
Folia Biologica
volume
51
issue
3
pages
62 - 67
publisher
Academy of Sciences of the Czech Republic
external identifiers
  • wos:000230552400002
  • pmid:16045237
  • scopus:22144485526
ISSN
0015-5500
language
English
LU publication?
yes
id
5d1ada0d-3b46-4ea9-bd15-8fe087374659 (old id 232329)
alternative location
http://fb.cuni.cz/Data/files/folia_biologica/volume_51_2005_3/FB2005A0012.pdf
date added to LUP
2007-08-08 09:36:27
date last changed
2017-01-01 06:41:11
@article{5d1ada0d-3b46-4ea9-bd15-8fe087374659,
  abstract     = {There is an increasing amount of knowledge on the cytotoxic properties of cyanotoxins, but relatively little is known regarding their fine specificity and mechanisms of action. In this study, we investigated the influence of microcystin-LR and AnTx-a on mouse B- and T-lymphocyte subpopulations in vitro. Cyanotoxins significantly decreased the cell viability after 4 and 24 h, compared to the untreated control. After 24 h exposure to microcystin-LR and anatoxin-a, the viability of splenocytes dropped to 23% and 57%, respectively. Our data demonstrate that microcystin-LR induced apoptosis specifically in mouse B cells, probably via the B-cell antigen receptor and mitochondrial pathway, while the T cells were not affected. AnTx-a showed cytotoxic effects on both lymphocyte subpopulations, but the effects were driven by mechanisms different from apoptosis. These findings demonstrate that the cyanotoxins could cause cytotoxic alterations in a variety of cell types different from the major targets, operating via distinct mechanisms.},
  author       = {Teneva, I and Mladenov, R and Popov, N and Dzhambazov, Balik},
  issn         = {0015-5500},
  keyword      = {cytotoxicity,microcystin-LR,anatoxin-a,mouse lymphocytes,apoptosis},
  language     = {eng},
  number       = {3},
  pages        = {62--67},
  publisher    = {Academy of Sciences of the Czech Republic},
  series       = {Folia Biologica},
  title        = {Cytotoxicity and apoptotic effects of microcystin-LR and anatoxin-a in mouse lymphocytes},
  volume       = {51},
  year         = {2005},
}