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Flow-through microdispenser for interfacing mu-HPLC to Raman and mid-IR spectroscopic detection

Surowiec, I; Baena, JR; Frank, J; Laurell, Thomas LU ; Nilsson, Johan LU ; Trojanowicz, M and Lendl, B (2005) In Journal of Chromatography A 1080(2). p.132-139
Abstract
A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique... (More)
A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with the reference spectra of corresponding components. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
flow through, HPLC, Raman microspectrometry, FTIR microscopy, microdispenser
in
Journal of Chromatography A
volume
1080
issue
2
pages
132 - 139
publisher
Elsevier
external identifiers
  • wos:000230238600005
  • pmid:16008051
  • scopus:20444490460
ISSN
0021-9673
DOI
10.1016/j.chroma.2005.04.082
language
English
LU publication?
yes
id
9332c25f-f26c-4b60-8af3-4056a2f3b740 (old id 233456)
date added to LUP
2007-08-13 16:09:55
date last changed
2017-05-28 04:22:51
@article{9332c25f-f26c-4b60-8af3-4056a2f3b740,
  abstract     = {A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with the reference spectra of corresponding components.},
  author       = {Surowiec, I and Baena, JR and Frank, J and Laurell, Thomas and Nilsson, Johan and Trojanowicz, M and Lendl, B},
  issn         = {0021-9673},
  keyword      = {flow through,HPLC,Raman microspectrometry,FTIR microscopy,microdispenser},
  language     = {eng},
  number       = {2},
  pages        = {132--139},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Flow-through microdispenser for interfacing mu-HPLC to Raman and mid-IR spectroscopic detection},
  url          = {http://dx.doi.org/10.1016/j.chroma.2005.04.082},
  volume       = {1080},
  year         = {2005},
}