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Modeling the organization of the WUSCHEL expression domain in the shoot apical meristem

Jönsson, Henrik LU ; Heisler, M; Reddy, GV; Agrawal, V; Gor, V; Shapiro, BE; Mjolsness, E and Meyerowitz, EM (2005) In Bioinformatics 21(Suppl 1). p.232-240
Abstract
Motivation: The above-ground tissues of higher plants are generated from a small region of cells situated at the plant apex called the shoot apical meristem. An important genetic control circuit modulating the size of the Arabidopsis thaliana meristem is a feed-back network between the CLAVATA3 and WUSCHEL genes. Although the expression patterns for these genes do not overlap, WUSCHEL activity is both necessary and sufficient (when expressed ectopically) for the induction of CLAVATA3 expression. However, upregulation of CLAVATA3 in conjunction with the receptor kinase CLAVATA1 results in the downregulation of WUSCHEL. Despite much work, experimental data for this network are incomplete and additional hypotheses are needed to explain the... (More)
Motivation: The above-ground tissues of higher plants are generated from a small region of cells situated at the plant apex called the shoot apical meristem. An important genetic control circuit modulating the size of the Arabidopsis thaliana meristem is a feed-back network between the CLAVATA3 and WUSCHEL genes. Although the expression patterns for these genes do not overlap, WUSCHEL activity is both necessary and sufficient (when expressed ectopically) for the induction of CLAVATA3 expression. However, upregulation of CLAVATA3 in conjunction with the receptor kinase CLAVATA1 results in the downregulation of WUSCHEL. Despite much work, experimental data for this network are incomplete and additional hypotheses are needed to explain the spatial locations and dynamics of these expression domains. Predictive mathematical models describing the system should provide a useful tool for investigating and discriminating among possible hypotheses, by determining which hypotheses best explain observed gene expression dynamics. Results: We are developing a method using in vivo live confocal microscopy to capture quantitative gene expression data and create templates for computational models. We present two models accounting for the organization of the WUSCHEL expression domain. Our preferred model uses a reaction-diffusion mechanism in which an activator induces WUSCHEL expression. This model is able to organize the WUSCHEL expression domain. In addition, the model predicts the dynamical reorganization seen in experiments where cells, including the WUSCHEL domain, are ablated, and it also predicts the spatial expansion of the WUSCHEL domain resulting from removal of the CLAVATA3 signal. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioinformatics
volume
21
issue
Suppl 1
pages
232 - 240
publisher
Oxford University Press
external identifiers
  • wos:000230273000026
  • scopus:26844530418
ISSN
1367-4803
DOI
10.1093/bioinformatics/bti1036
language
English
LU publication?
yes
id
88f49726-cdd4-4759-94c3-54d0542fbca2 (old id 233606)
date added to LUP
2007-08-17 16:52:10
date last changed
2017-10-01 03:44:27
@article{88f49726-cdd4-4759-94c3-54d0542fbca2,
  abstract     = {Motivation: The above-ground tissues of higher plants are generated from a small region of cells situated at the plant apex called the shoot apical meristem. An important genetic control circuit modulating the size of the Arabidopsis thaliana meristem is a feed-back network between the CLAVATA3 and WUSCHEL genes. Although the expression patterns for these genes do not overlap, WUSCHEL activity is both necessary and sufficient (when expressed ectopically) for the induction of CLAVATA3 expression. However, upregulation of CLAVATA3 in conjunction with the receptor kinase CLAVATA1 results in the downregulation of WUSCHEL. Despite much work, experimental data for this network are incomplete and additional hypotheses are needed to explain the spatial locations and dynamics of these expression domains. Predictive mathematical models describing the system should provide a useful tool for investigating and discriminating among possible hypotheses, by determining which hypotheses best explain observed gene expression dynamics. Results: We are developing a method using in vivo live confocal microscopy to capture quantitative gene expression data and create templates for computational models. We present two models accounting for the organization of the WUSCHEL expression domain. Our preferred model uses a reaction-diffusion mechanism in which an activator induces WUSCHEL expression. This model is able to organize the WUSCHEL expression domain. In addition, the model predicts the dynamical reorganization seen in experiments where cells, including the WUSCHEL domain, are ablated, and it also predicts the spatial expansion of the WUSCHEL domain resulting from removal of the CLAVATA3 signal.},
  author       = {Jönsson, Henrik and Heisler, M and Reddy, GV and Agrawal, V and Gor, V and Shapiro, BE and Mjolsness, E and Meyerowitz, EM},
  issn         = {1367-4803},
  language     = {eng},
  number       = {Suppl 1},
  pages        = {232--240},
  publisher    = {Oxford University Press},
  series       = {Bioinformatics},
  title        = {Modeling the organization of the WUSCHEL expression domain in the shoot apical meristem},
  url          = {http://dx.doi.org/10.1093/bioinformatics/bti1036},
  volume       = {21},
  year         = {2005},
}