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Detection of neurocan in cerebrospinal fluid.

Rauch, Uwe LU (2012) In Methods in Molecular Biology 836. p.87-95
Abstract
Cerebrospinal fluid (CFS) is the most easily accessible component of the human central nervous system and has been successfully used for the analysis of disease-associated molecular imbalances, particularly for extracellular matrix components. Alterations in the presence of the nervous system-associated chondroitin sulfate proteoglycan neurocan had been reported from active multiple sclerosis lesions. Neurocan could be detected as a component of human CFS after enrichment of proteoglycans by anion exchange chromatography from pooled liquor as well as individual 300 μL samples by Western blot. However, a general alteration in neurocan levels in CFS sample with high immunoglobulin content could not be demonstrated. To further reduce the... (More)
Cerebrospinal fluid (CFS) is the most easily accessible component of the human central nervous system and has been successfully used for the analysis of disease-associated molecular imbalances, particularly for extracellular matrix components. Alterations in the presence of the nervous system-associated chondroitin sulfate proteoglycan neurocan had been reported from active multiple sclerosis lesions. Neurocan could be detected as a component of human CFS after enrichment of proteoglycans by anion exchange chromatography from pooled liquor as well as individual 300 μL samples by Western blot. However, a general alteration in neurocan levels in CFS sample with high immunoglobulin content could not be demonstrated. To further reduce the sample size, the development of a PG capturing assay based on polybrene-coated 96-well plates was initiated. This approach could be an interesting alternative option for the analysis of PGs in biological fluid and tissue samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
836
pages
87 - 95
publisher
Springer
external identifiers
  • pmid:22252629
  • scopus:84856317740
ISSN
1940-6029
DOI
10.1007/978-1-61779-498-8_6
language
English
LU publication?
yes
id
3e98271d-8620-4eb4-9a70-0c0af718da2a (old id 2336275)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22252629?dopt=Abstract
date added to LUP
2012-02-01 21:48:56
date last changed
2017-01-01 07:34:05
@article{3e98271d-8620-4eb4-9a70-0c0af718da2a,
  abstract     = {Cerebrospinal fluid (CFS) is the most easily accessible component of the human central nervous system and has been successfully used for the analysis of disease-associated molecular imbalances, particularly for extracellular matrix components. Alterations in the presence of the nervous system-associated chondroitin sulfate proteoglycan neurocan had been reported from active multiple sclerosis lesions. Neurocan could be detected as a component of human CFS after enrichment of proteoglycans by anion exchange chromatography from pooled liquor as well as individual 300 μL samples by Western blot. However, a general alteration in neurocan levels in CFS sample with high immunoglobulin content could not be demonstrated. To further reduce the sample size, the development of a PG capturing assay based on polybrene-coated 96-well plates was initiated. This approach could be an interesting alternative option for the analysis of PGs in biological fluid and tissue samples.},
  author       = {Rauch, Uwe},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {87--95},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Detection of neurocan in cerebrospinal fluid.},
  url          = {http://dx.doi.org/10.1007/978-1-61779-498-8_6},
  volume       = {836},
  year         = {2012},
}