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Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry.

Végvári, Ákos LU ; Rezeli, Melinda LU ; Sihlbom, Carina; Häkkinen, Jari LU ; Carlsohn, Elisabet; Malm, Johan LU ; Lilja, Hans LU ; Laurell, Thomas LU and Marko-Varga, György LU (2012) In Clinical Biochemistry 45(4-5). p.331-338
Abstract
OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels.... (More)
OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels. Multivariate analysis of clinical data revealed well-separated patient groups. CONCLUSIONS: We demonstrated that PSA in seminal plasma occurs in several isoforms, yet not all were detectable using an antibody based clinical routine method. The heterogeneity of PSA expression might be of clinical significance, by an improved patient phenotyping. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ESI-LTQ FT-ICR, MALDI LTQ Orbitrap XL, infertility, seminal plasma, prostate specific antigen, isoform
in
Clinical Biochemistry
volume
45
issue
4-5
pages
331 - 338
publisher
Elsevier
external identifiers
  • wos:000302844500012
  • pmid:22209970
  • scopus:84857655838
ISSN
1873-2933
DOI
10.1016/j.clinbiochem.2011.11.018
language
English
LU publication?
yes
id
a84afa89-e40e-43fe-9a26-4aae21614c7a (old id 2336781)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22209970?dopt=Abstract
date added to LUP
2012-02-01 19:03:47
date last changed
2017-10-01 03:10:54
@article{a84afa89-e40e-43fe-9a26-4aae21614c7a,
  abstract     = {OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels. Multivariate analysis of clinical data revealed well-separated patient groups. CONCLUSIONS: We demonstrated that PSA in seminal plasma occurs in several isoforms, yet not all were detectable using an antibody based clinical routine method. The heterogeneity of PSA expression might be of clinical significance, by an improved patient phenotyping.},
  author       = {Végvári, Ákos and Rezeli, Melinda and Sihlbom, Carina and Häkkinen, Jari and Carlsohn, Elisabet and Malm, Johan and Lilja, Hans and Laurell, Thomas and Marko-Varga, György},
  issn         = {1873-2933},
  keyword      = {ESI-LTQ FT-ICR,MALDI LTQ Orbitrap XL,infertility,seminal plasma,prostate specific antigen,isoform},
  language     = {eng},
  number       = {4-5},
  pages        = {331--338},
  publisher    = {Elsevier},
  series       = {Clinical Biochemistry},
  title        = {Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry.},
  url          = {http://dx.doi.org/10.1016/j.clinbiochem.2011.11.018},
  volume       = {45},
  year         = {2012},
}