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Links between methanotroph community composition and CH(4) oxidation in a pine forest soil

Bengtson, Per LU ; Basiliko, Nathan; Dumont, Marc G; Hills, Melissa; Murrell, J Colin; Roy, Real and Grayston, Sue J (2009) In FEMS Microbiology Ecology 70(3). p.356-366
Abstract
The main gap in our knowledge about what determines the rate of CH(4) oxidation in forest soils is the biology of the microorganisms involved, the identity of which remains unclear. In this study, we used stable-isotope probing (SIP) following (13)CH(4) incorporation into phospholipid fatty acids (PLFAs) and DNA/RNA, and sequencing of methane mono-oxygenase (pmoA) genes, to identify the influence of variation in community composition on CH(4) oxidation rates. The rates of (13)C incorporation into PLFAs differed between horizons, with low (13)C incorporation in the organic soil and relatively high (13)C incorporation into the two mineral horizons. The microbial community composition of the methanotrophs incorporating the (13)C label also... (More)
The main gap in our knowledge about what determines the rate of CH(4) oxidation in forest soils is the biology of the microorganisms involved, the identity of which remains unclear. In this study, we used stable-isotope probing (SIP) following (13)CH(4) incorporation into phospholipid fatty acids (PLFAs) and DNA/RNA, and sequencing of methane mono-oxygenase (pmoA) genes, to identify the influence of variation in community composition on CH(4) oxidation rates. The rates of (13)C incorporation into PLFAs differed between horizons, with low (13)C incorporation in the organic soil and relatively high (13)C incorporation into the two mineral horizons. The microbial community composition of the methanotrophs incorporating the (13)C label also differed between horizons, and statistical analyses suggested that the methanotroph community composition was a major cause of variation in CH(4) oxidation rates. Both PLFA and pmoA-based data indicated that CH(4) oxidizers in this soil belong to the uncultivated 'upland soil cluster alpha'. CH(4) oxidation potential exhibited the opposite pattern to (13)C incorporation, suggesting that CH(4) oxidation potential assays may correlate poorly with in situ oxidation rates. The DNA/RNA-SIP assay was not successful, most likely due to insufficient (13)C-incorporation into DNA/RNA. The limitations of the technique are briefly discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
methane-oxidizing bacteria, methanotroph, nucleic acid, stable-isotope probing, phospholipid fatty acid, methane mono-oxygenase
in
FEMS Microbiology Ecology
volume
70
issue
3
pages
356 - 366
publisher
Elsevier
external identifiers
  • scopus:70449123858
ISSN
1574-6941
DOI
10.1111/j.1574-6941.2009.00751.x
language
English
LU publication?
yes
id
c866e393-1df6-4585-9255-21f7d38c66e2 (old id 2339704)
date added to LUP
2012-02-13 12:54:29
date last changed
2017-12-10 03:43:53
@article{c866e393-1df6-4585-9255-21f7d38c66e2,
  abstract     = {The main gap in our knowledge about what determines the rate of CH(4) oxidation in forest soils is the biology of the microorganisms involved, the identity of which remains unclear. In this study, we used stable-isotope probing (SIP) following (13)CH(4) incorporation into phospholipid fatty acids (PLFAs) and DNA/RNA, and sequencing of methane mono-oxygenase (pmoA) genes, to identify the influence of variation in community composition on CH(4) oxidation rates. The rates of (13)C incorporation into PLFAs differed between horizons, with low (13)C incorporation in the organic soil and relatively high (13)C incorporation into the two mineral horizons. The microbial community composition of the methanotrophs incorporating the (13)C label also differed between horizons, and statistical analyses suggested that the methanotroph community composition was a major cause of variation in CH(4) oxidation rates. Both PLFA and pmoA-based data indicated that CH(4) oxidizers in this soil belong to the uncultivated 'upland soil cluster alpha'. CH(4) oxidation potential exhibited the opposite pattern to (13)C incorporation, suggesting that CH(4) oxidation potential assays may correlate poorly with in situ oxidation rates. The DNA/RNA-SIP assay was not successful, most likely due to insufficient (13)C-incorporation into DNA/RNA. The limitations of the technique are briefly discussed.},
  author       = {Bengtson, Per and Basiliko, Nathan and Dumont, Marc G and Hills, Melissa and Murrell, J Colin and Roy, Real and Grayston, Sue J},
  issn         = {1574-6941},
  keyword      = {methane-oxidizing bacteria,methanotroph,nucleic acid,stable-isotope probing,phospholipid fatty acid,methane mono-oxygenase},
  language     = {eng},
  number       = {3},
  pages        = {356--366},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Ecology},
  title        = {Links between methanotroph community composition and CH(4) oxidation in a pine forest soil},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2009.00751.x},
  volume       = {70},
  year         = {2009},
}