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Human neurospheres: From stained sections to three-dimensional assembly

Monni, Emanuela LU ; Congiu, Terenzio; Massa, Denise; Nat, Roxana and Diana, Andrea (2011) In Translational Neuroscience 2(1). p.43-48
Abstract
Human neurospheres are free-floating spherical clusters generated from a single neural stem cell and comprising cells at different stages of maturation in the neuronal and glial lineages. Although recent findings have disproved the original idea of clonally derived neurospheres according to the paradigm of one stem cell - one neurosphere, they still represent a valid model for growing neural stem cell cultures in vitro. While the immunocytochemical approach to the identification of stem cells, progenitor cells, and mature cells has been extensively used, scant data are available about the ultrastructural arrangement of different cell types within the neurosphere. This paper provides, by means of scanning electron microscopy, some new... (More)
Human neurospheres are free-floating spherical clusters generated from a single neural stem cell and comprising cells at different stages of maturation in the neuronal and glial lineages. Although recent findings have disproved the original idea of clonally derived neurospheres according to the paradigm of one stem cell - one neurosphere, they still represent a valid model for growing neural stem cell cultures in vitro. While the immunocytochemical approach to the identification of stem cells, progenitor cells, and mature cells has been extensively used, scant data are available about the ultrastructural arrangement of different cell types within the neurosphere. This paper provides, by means of scanning electron microscopy, some new insights into the three-dimensional assembly of human neurospheres, trying to correlate some parameters such as cell density, shape and growing strategies with the immunolocalization of some antigens such as nestin, GFAP, alpha-internexin and beta III-tubulin. The major findings from this study are: a) regardless of the stage of in vitro maturation, the growth of the spheres is the result of mitotic divisions producing the aspect of an irregular budding mechanism in the outermost layer look like; b) analysis of the volumetric composition of the inner core has revealed the presence of two alternative shape pattern (pyramidal vs rounded cells) possibly related to both the ongoing maturation stages and GFAP and internexin expression. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Human neurospheres, Neural stem cells, Scanning electron microscopy, Immunocytochemistry
in
Translational Neuroscience
volume
2
issue
1
pages
43 - 48
publisher
Versita
external identifiers
  • wos:000298886500006
  • scopus:80051655845
ISSN
2081-6936
DOI
10.2478/s13380-011-0007-4
language
English
LU publication?
yes
id
dc1202c3-c32b-405e-a13e-e56faa7b63ee (old id 2348660)
date added to LUP
2012-03-01 11:22:10
date last changed
2017-03-05 03:10:27
@article{dc1202c3-c32b-405e-a13e-e56faa7b63ee,
  abstract     = {Human neurospheres are free-floating spherical clusters generated from a single neural stem cell and comprising cells at different stages of maturation in the neuronal and glial lineages. Although recent findings have disproved the original idea of clonally derived neurospheres according to the paradigm of one stem cell - one neurosphere, they still represent a valid model for growing neural stem cell cultures in vitro. While the immunocytochemical approach to the identification of stem cells, progenitor cells, and mature cells has been extensively used, scant data are available about the ultrastructural arrangement of different cell types within the neurosphere. This paper provides, by means of scanning electron microscopy, some new insights into the three-dimensional assembly of human neurospheres, trying to correlate some parameters such as cell density, shape and growing strategies with the immunolocalization of some antigens such as nestin, GFAP, alpha-internexin and beta III-tubulin. The major findings from this study are: a) regardless of the stage of in vitro maturation, the growth of the spheres is the result of mitotic divisions producing the aspect of an irregular budding mechanism in the outermost layer look like; b) analysis of the volumetric composition of the inner core has revealed the presence of two alternative shape pattern (pyramidal vs rounded cells) possibly related to both the ongoing maturation stages and GFAP and internexin expression.},
  author       = {Monni, Emanuela and Congiu, Terenzio and Massa, Denise and Nat, Roxana and Diana, Andrea},
  issn         = {2081-6936},
  keyword      = {Human neurospheres,Neural stem cells,Scanning electron microscopy,Immunocytochemistry},
  language     = {eng},
  number       = {1},
  pages        = {43--48},
  publisher    = {Versita},
  series       = {Translational Neuroscience},
  title        = {Human neurospheres: From stained sections to three-dimensional assembly},
  url          = {http://dx.doi.org/10.2478/s13380-011-0007-4},
  volume       = {2},
  year         = {2011},
}