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Formulation and in vitro/in vivo evaluation of combining DNA repair and immune enhancing nutritional supplements

Pero, Ronald LU ; Amiri, Amir LU ; Sheng, Yezhou LU ; Welther, M and Rich, M (2005) In Phytomedicine 12(4). p.255-263
Abstract
Combining nutritional supplements to achieve synergistic benefit is a common practice in the nutraceutical industry. However, establishing. added health benefit from a combination of natural ingredients is often assumed, untested and without regard to the principle of metabolic competition between the active components. Here, we report on the combination of a cat's claw water extract (C-Med-100, carboxy alkyl esters = active ingredients) + medicinal mushroom extracts (Cordyceps sinensis, Grifola blazei, Grifola frondosa, Trametes versicolor and Ganoderma lucidum, polysaccharides = active ingredients) + nicotinamide + zinc into a formulation designed to optimize different modes of immunostimulatory action, and yet that would avoid metabolic... (More)
Combining nutritional supplements to achieve synergistic benefit is a common practice in the nutraceutical industry. However, establishing. added health benefit from a combination of natural ingredients is often assumed, untested and without regard to the principle of metabolic competition between the active components. Here, we report on the combination of a cat's claw water extract (C-Med-100, carboxy alkyl esters = active ingredients) + medicinal mushroom extracts (Cordyceps sinensis, Grifola blazei, Grifola frondosa, Trametes versicolor and Ganoderma lucidum, polysaccharides = active ingredients) + nicotinamide + zinc into a formulation designed to optimize different modes of immunostimulatory action, and yet that would avoid metabolic antioxidant competition yielding less than expected efficacious effects. Isobole curve analyses of these two active classes of ingredients determined by growth inhibition of HL-60 human leukemic cells in vitro confirmed they were indeed synergistic when in combination, and not metabolically competitive. Furthermore, an in vivo study showed significant health benefit for 14 subjects treated for 4 weeks with the unique C-Med-100/mushroom extract formulation in that they had reduced pain, reduced fatigue, weight loss and a reduced presence of DNA damage in peripheral blood assessed by (8-OH) guanine DNA ad, ducts and elevation in serum protein thiols. Because this broad-based panel of clinical parameters indicating clinical efficacy has never been demonstrated before for either of the active ingredients evaluated alone in humans, these data were taken as strong evidence that the combination of C-Med-100 + mushroom extracts + nicotinamide + zinc gave additive or synergistic effects to health benefit, and thus supported no efficacious limits from metabolic competition regarding this particular formulation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nutritional, Chinese mushroom extracts, cat's claw C-Med-100 extract, additive/synergistic efficacy, weight loss
in
Phytomedicine
volume
12
issue
4
pages
255 - 263
publisher
Urban & Fischer Verlag
external identifiers
  • pmid:15898702
  • wos:000229702400001
  • scopus:16344375888
ISSN
0944-7113
DOI
10.1016/j.phymed.2004.01.008
language
English
LU publication?
yes
id
18500fb4-cb4e-4014-953c-ea4f63bdefff (old id 236401)
date added to LUP
2007-08-13 14:12:40
date last changed
2017-01-01 07:12:11
@article{18500fb4-cb4e-4014-953c-ea4f63bdefff,
  abstract     = {Combining nutritional supplements to achieve synergistic benefit is a common practice in the nutraceutical industry. However, establishing. added health benefit from a combination of natural ingredients is often assumed, untested and without regard to the principle of metabolic competition between the active components. Here, we report on the combination of a cat's claw water extract (C-Med-100, carboxy alkyl esters = active ingredients) + medicinal mushroom extracts (Cordyceps sinensis, Grifola blazei, Grifola frondosa, Trametes versicolor and Ganoderma lucidum, polysaccharides = active ingredients) + nicotinamide + zinc into a formulation designed to optimize different modes of immunostimulatory action, and yet that would avoid metabolic antioxidant competition yielding less than expected efficacious effects. Isobole curve analyses of these two active classes of ingredients determined by growth inhibition of HL-60 human leukemic cells in vitro confirmed they were indeed synergistic when in combination, and not metabolically competitive. Furthermore, an in vivo study showed significant health benefit for 14 subjects treated for 4 weeks with the unique C-Med-100/mushroom extract formulation in that they had reduced pain, reduced fatigue, weight loss and a reduced presence of DNA damage in peripheral blood assessed by (8-OH) guanine DNA ad, ducts and elevation in serum protein thiols. Because this broad-based panel of clinical parameters indicating clinical efficacy has never been demonstrated before for either of the active ingredients evaluated alone in humans, these data were taken as strong evidence that the combination of C-Med-100 + mushroom extracts + nicotinamide + zinc gave additive or synergistic effects to health benefit, and thus supported no efficacious limits from metabolic competition regarding this particular formulation.},
  author       = {Pero, Ronald and Amiri, Amir and Sheng, Yezhou and Welther, M and Rich, M},
  issn         = {0944-7113},
  keyword      = {nutritional,Chinese mushroom extracts,cat's claw C-Med-100 extract,additive/synergistic efficacy,weight loss},
  language     = {eng},
  number       = {4},
  pages        = {255--263},
  publisher    = {Urban & Fischer Verlag},
  series       = {Phytomedicine},
  title        = {Formulation and in vitro/in vivo evaluation of combining DNA repair and immune enhancing nutritional supplements},
  url          = {http://dx.doi.org/10.1016/j.phymed.2004.01.008},
  volume       = {12},
  year         = {2005},
}