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Preparation of Calcium Alginate Nanoparticles Using Water-in-Oil (W/O) Nanoemulsions.

Machado, Alexandra LU ; Lundberg, Dan LU ; Ribeiro, António; Veiga, Francisco; Lindman, Björn LU ; Miguel, Maria LU and Olsson, Ulf LU (2012) In Langmuir 28(9). p.4131-4141
Abstract
A procedure for the preparation of calcium alginate nanoparticles in the aqueous phase of water-in-oil (W/O) nanoemulsions was developed. The emulsions were produced from mixtures of the nonionic surfactant tetraethylene glycol monododecyl ether (C(12)E(4)), decane, and aqueous solutions of up to 2 wt % sodium alginate by means of the phase inversion temperature (PIT) emulsification method. This method allows the preparation of finely dispersed emulsions without a large input of mechanical energy. With alginate concentrations of 1-2 wt % in the aqueous phase, emulsions showed good stability against Ostwald ripening and narrow, monomodal distributions of droplets with radii <100 nm. Gelation of the alginate was induced by the addition of... (More)
A procedure for the preparation of calcium alginate nanoparticles in the aqueous phase of water-in-oil (W/O) nanoemulsions was developed. The emulsions were produced from mixtures of the nonionic surfactant tetraethylene glycol monododecyl ether (C(12)E(4)), decane, and aqueous solutions of up to 2 wt % sodium alginate by means of the phase inversion temperature (PIT) emulsification method. This method allows the preparation of finely dispersed emulsions without a large input of mechanical energy. With alginate concentrations of 1-2 wt % in the aqueous phase, emulsions showed good stability against Ostwald ripening and narrow, monomodal distributions of droplets with radii <100 nm. Gelation of the alginate was induced by the addition of aqueous CaCl(2) to the emulsions under stirring, and particles formed were collected using a simple procedure based on extraction of the surfactant on addition of excess oil. The final particles were characterized using cryo-transmission electron microscopy (cryo-TEM) and dynamic light scattering (DLS). They were found to be essentially spherical with a homogeneous interior, and their size was similar to that of the initial emulsion droplets. The herein presented "low-energy" method for preparation of biocompatible nanoparticles has the potential to be used in various applications, e.g., for the encapsulation of sensitive biomacromolecules. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
28
issue
9
pages
4131 - 4141
publisher
The American Chemical Society
external identifiers
  • wos:000301038000013
  • pmid:22296569
  • scopus:84857848619
ISSN
0743-7463
DOI
10.1021/la204944j
language
English
LU publication?
yes
id
a847d384-38b8-40f3-b3c9-9278cf69636e (old id 2367473)
date added to LUP
2012-03-06 17:22:24
date last changed
2017-11-05 03:02:34
@article{a847d384-38b8-40f3-b3c9-9278cf69636e,
  abstract     = {A procedure for the preparation of calcium alginate nanoparticles in the aqueous phase of water-in-oil (W/O) nanoemulsions was developed. The emulsions were produced from mixtures of the nonionic surfactant tetraethylene glycol monododecyl ether (C(12)E(4)), decane, and aqueous solutions of up to 2 wt % sodium alginate by means of the phase inversion temperature (PIT) emulsification method. This method allows the preparation of finely dispersed emulsions without a large input of mechanical energy. With alginate concentrations of 1-2 wt % in the aqueous phase, emulsions showed good stability against Ostwald ripening and narrow, monomodal distributions of droplets with radii &lt;100 nm. Gelation of the alginate was induced by the addition of aqueous CaCl(2) to the emulsions under stirring, and particles formed were collected using a simple procedure based on extraction of the surfactant on addition of excess oil. The final particles were characterized using cryo-transmission electron microscopy (cryo-TEM) and dynamic light scattering (DLS). They were found to be essentially spherical with a homogeneous interior, and their size was similar to that of the initial emulsion droplets. The herein presented "low-energy" method for preparation of biocompatible nanoparticles has the potential to be used in various applications, e.g., for the encapsulation of sensitive biomacromolecules.},
  author       = {Machado, Alexandra and Lundberg, Dan and Ribeiro, António and Veiga, Francisco and Lindman, Björn and Miguel, Maria and Olsson, Ulf},
  issn         = {0743-7463},
  language     = {eng},
  number       = {9},
  pages        = {4131--4141},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Preparation of Calcium Alginate Nanoparticles Using Water-in-Oil (W/O) Nanoemulsions.},
  url          = {http://dx.doi.org/10.1021/la204944j},
  volume       = {28},
  year         = {2012},
}