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Interference elimination in glutamate monitoring with chip integrated enzyme microreactors

Collins, A.; Mikeladze, E.; Bengtsson, M.; Kokaia, M.; Laurell, Thomas LU and Csöregi, Elisabeth LU (2001) In Electroanalysis 13(6). p.425-431
Abstract
On-chip enzyme reactors are often used in medical/pharmaceutical analysis due to their inherent advantages, such as high sample throughput, low reagent consumption, stability, reproducibility and low cost. The present work describes a different application of such microreactors, namely, elimination of interfering ascorbate signals in glutamate monitoring using ascorbate oxidase modified silicon chip microreactors of different sizes (5.3 and 0.95 muL). Glutamate was monitored with a previously developed redox hydrogel integrated bienzyme electrode, based on coupled glutamate oxidase and horseradish peroxidase, inserted in a miniaturized flow cell operated at -50 mV (vs. Ag/AgCl). The developed on-line analysis system was characterized with... (More)
On-chip enzyme reactors are often used in medical/pharmaceutical analysis due to their inherent advantages, such as high sample throughput, low reagent consumption, stability, reproducibility and low cost. The present work describes a different application of such microreactors, namely, elimination of interfering ascorbate signals in glutamate monitoring using ascorbate oxidase modified silicon chip microreactors of different sizes (5.3 and 0.95 muL). Glutamate was monitored with a previously developed redox hydrogel integrated bienzyme electrode, based on coupled glutamate oxidase and horseradish peroxidase, inserted in a miniaturized flow cell operated at -50 mV (vs. Ag/AgCl). The developed on-line analysis system was characterized with regard to dilution effects, detection limit, response time and interference ability using model solutions and real samples. Off-line in vivo glutamate measurements could be made by injecting rat brain microdialysate samples collected before and after KCl stimulation without any interference of ascorbate, I Within the studied flow rate range (2-25 muL/min), 1 mM and 200 muM ascorbate could he totally eliminated using the larger and the mailer microreactor, respectively. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glutamate, amperometric biosensor, ascorbate interference elimination, immobilized enzyme reactors, FLOW-INJECTION-ANALYSIS, POROUS SILICON, AMPEROMETRIC DETERMINATION, EXTRACELLULAR GLUTAMATE, GLUCOSE SENSOR, BIOSENSOR, OXIDASE, ELECTRODE, REACTORS, DEHYDROGENASE
in
Electroanalysis
volume
13
issue
6
pages
425 - 431
publisher
John Wiley & Sons
external identifiers
  • wos:000168509300001
  • scopus:0035013459
ISSN
1040-0397
DOI
language
English
LU publication?
yes
id
59357481-e0ac-42ef-b1c8-f832ebf83961 (old id 2376283)
date added to LUP
2012-03-23 09:48:50
date last changed
2018-05-29 10:25:44
@article{59357481-e0ac-42ef-b1c8-f832ebf83961,
  abstract     = {On-chip enzyme reactors are often used in medical/pharmaceutical analysis due to their inherent advantages, such as high sample throughput, low reagent consumption, stability, reproducibility and low cost. The present work describes a different application of such microreactors, namely, elimination of interfering ascorbate signals in glutamate monitoring using ascorbate oxidase modified silicon chip microreactors of different sizes (5.3 and 0.95 muL). Glutamate was monitored with a previously developed redox hydrogel integrated bienzyme electrode, based on coupled glutamate oxidase and horseradish peroxidase, inserted in a miniaturized flow cell operated at -50 mV (vs. Ag/AgCl). The developed on-line analysis system was characterized with regard to dilution effects, detection limit, response time and interference ability using model solutions and real samples. Off-line in vivo glutamate measurements could be made by injecting rat brain microdialysate samples collected before and after KCl stimulation without any interference of ascorbate, I Within the studied flow rate range (2-25 muL/min), 1 mM and 200 muM ascorbate could he totally eliminated using the larger and the mailer microreactor, respectively.},
  author       = {Collins, A. and Mikeladze, E. and Bengtsson, M. and Kokaia, M. and Laurell, Thomas and Csöregi, Elisabeth},
  issn         = {1040-0397},
  keyword      = {glutamate,amperometric biosensor,ascorbate interference elimination,immobilized enzyme reactors,FLOW-INJECTION-ANALYSIS,POROUS SILICON,AMPEROMETRIC DETERMINATION,EXTRACELLULAR GLUTAMATE,GLUCOSE SENSOR,BIOSENSOR,OXIDASE,ELECTRODE,REACTORS,DEHYDROGENASE},
  language     = {eng},
  number       = {6},
  pages        = {425--431},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Interference elimination in glutamate monitoring with chip integrated enzyme microreactors},
  url          = {http://dx.doi.org/},
  volume       = {13},
  year         = {2001},
}