Interference elimination in glutamate monitoring with chip integrated enzyme microreactors
(2001) In Electroanalysis 13(6). p.425-431- Abstract
- On-chip enzyme reactors are often used in medical/pharmaceutical analysis due to their inherent advantages, such as high sample throughput, low reagent consumption, stability, reproducibility and low cost. The present work describes a different application of such microreactors, namely, elimination of interfering ascorbate signals in glutamate monitoring using ascorbate oxidase modified silicon chip microreactors of different sizes (5.3 and 0.95 muL). Glutamate was monitored with a previously developed redox hydrogel integrated bienzyme electrode, based on coupled glutamate oxidase and horseradish peroxidase, inserted in a miniaturized flow cell operated at -50 mV (vs. Ag/AgCl). The developed on-line analysis system was characterized with... (More)
- On-chip enzyme reactors are often used in medical/pharmaceutical analysis due to their inherent advantages, such as high sample throughput, low reagent consumption, stability, reproducibility and low cost. The present work describes a different application of such microreactors, namely, elimination of interfering ascorbate signals in glutamate monitoring using ascorbate oxidase modified silicon chip microreactors of different sizes (5.3 and 0.95 muL). Glutamate was monitored with a previously developed redox hydrogel integrated bienzyme electrode, based on coupled glutamate oxidase and horseradish peroxidase, inserted in a miniaturized flow cell operated at -50 mV (vs. Ag/AgCl). The developed on-line analysis system was characterized with regard to dilution effects, detection limit, response time and interference ability using model solutions and real samples. Off-line in vivo glutamate measurements could be made by injecting rat brain microdialysate samples collected before and after KCl stimulation without any interference of ascorbate, I Within the studied flow rate range (2-25 muL/min), 1 mM and 200 muM ascorbate could he totally eliminated using the larger and the mailer microreactor, respectively. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2376283
- author
- Collins, A. ; Mikeladze, E. ; Bengtsson, M. ; Kokaia, M. ; Laurell, Thomas LU and Csöregi, Elisabeth LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glutamate, amperometric biosensor, ascorbate interference elimination, immobilized enzyme reactors, FLOW-INJECTION-ANALYSIS, POROUS SILICON, AMPEROMETRIC DETERMINATION, EXTRACELLULAR GLUTAMATE, GLUCOSE SENSOR, BIOSENSOR, OXIDASE, ELECTRODE, REACTORS, DEHYDROGENASE
- in
- Electroanalysis
- volume
- 13
- issue
- 6
- pages
- 425 - 431
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000168509300001
- scopus:0035013459
- ISSN
- 1040-0397
- DOI
- 10.1002/1521-4109(200104)13:6<425::aid-elan425>3.0.co;2-5
- language
- English
- LU publication?
- yes
- id
- 59357481-e0ac-42ef-b1c8-f832ebf83961 (old id 2376283)
- date added to LUP
- 2016-04-01 16:11:58
- date last changed
- 2022-03-30 06:15:45
@article{59357481-e0ac-42ef-b1c8-f832ebf83961, abstract = {{On-chip enzyme reactors are often used in medical/pharmaceutical analysis due to their inherent advantages, such as high sample throughput, low reagent consumption, stability, reproducibility and low cost. The present work describes a different application of such microreactors, namely, elimination of interfering ascorbate signals in glutamate monitoring using ascorbate oxidase modified silicon chip microreactors of different sizes (5.3 and 0.95 muL). Glutamate was monitored with a previously developed redox hydrogel integrated bienzyme electrode, based on coupled glutamate oxidase and horseradish peroxidase, inserted in a miniaturized flow cell operated at -50 mV (vs. Ag/AgCl). The developed on-line analysis system was characterized with regard to dilution effects, detection limit, response time and interference ability using model solutions and real samples. Off-line in vivo glutamate measurements could be made by injecting rat brain microdialysate samples collected before and after KCl stimulation without any interference of ascorbate, I Within the studied flow rate range (2-25 muL/min), 1 mM and 200 muM ascorbate could he totally eliminated using the larger and the mailer microreactor, respectively.}}, author = {{Collins, A. and Mikeladze, E. and Bengtsson, M. and Kokaia, M. and Laurell, Thomas and Csöregi, Elisabeth}}, issn = {{1040-0397}}, keywords = {{glutamate; amperometric biosensor; ascorbate interference elimination; immobilized enzyme reactors; FLOW-INJECTION-ANALYSIS; POROUS SILICON; AMPEROMETRIC DETERMINATION; EXTRACELLULAR GLUTAMATE; GLUCOSE SENSOR; BIOSENSOR; OXIDASE; ELECTRODE; REACTORS; DEHYDROGENASE}}, language = {{eng}}, number = {{6}}, pages = {{425--431}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Electroanalysis}}, title = {{Interference elimination in glutamate monitoring with chip integrated enzyme microreactors}}, url = {{http://dx.doi.org/10.1002/1521-4109(200104)13:6<425::aid-elan425>3.0.co;2-5}}, doi = {{10.1002/1521-4109(200104)13:6<425::aid-elan425>3.0.co;2-5}}, volume = {{13}}, year = {{2001}}, }