Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: II. Biological applications
(2001) In Electrophoresis 22(18). p.3984-3992- Abstract
- A novel disposable high-density matrix assisted laser desorption/ionization (MALDI) target plate made either from polymethylmethacrylate (PMMA) or polycarbonate (PC) is presented where thousands (1200-1600) of samples can be deposited and subsequently, analyzed by MALDI-time of flight (TOF) mass spectrometry. Good reproducibility, was obtained across the plate regardless of position on the target plate with a relative standard deviation (RSD) on the peak intensity of typically 30% calculated from data generated by analysis of a 10 nM peptide mixture of angiotensin. I, II, III and bradykinin. The nanovial array format combined with microdispensing technology makes It possible to carry out in-vial, chemistry on deposited samples. This is... (More)
- A novel disposable high-density matrix assisted laser desorption/ionization (MALDI) target plate made either from polymethylmethacrylate (PMMA) or polycarbonate (PC) is presented where thousands (1200-1600) of samples can be deposited and subsequently, analyzed by MALDI-time of flight (TOF) mass spectrometry. Good reproducibility, was obtained across the plate regardless of position on the target plate with a relative standard deviation (RSD) on the peak intensity of typically 30% calculated from data generated by analysis of a 10 nM peptide mixture of angiotensin. I, II, III and bradykinin. The nanovial array format combined with microdispensing technology makes It possible to carry out in-vial, chemistry on deposited samples. This is demonstrated by the analysis of peptides from beta -casein and subsequent in-vial dephosphorylation of its phosphopeptides at 10 fmol levels by microdispensing of alkaline phosphatase, into the nanovial. The mass spectra obtained from these polymeric targets provides can also be used in high sensitivity applications as shown by peptide mass fingerprinting of human fibroblast proteins separated by two-dimensional gel, electrophoresis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2376314
- author
- Ekstrom, S. ; Nilsson, J. ; Helldin, G. ; Laurell, Thomas LU and Marko-Varga, György LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- polymer high-density matrix assisted laser desorption, ionization target, plates, phosphoproteins, peptide mapping, array, microdispensing, IMMOBILIZED PH GRADIENTS, PROTEIN IDENTIFICATION, SAMPLE PREPARATION, 2-DIMENSIONAL ELECTROPHORESIS, LIQUID-CHROMATOGRAPHY, SEQUENCE, DATABASES, PURIFICATION, PROTEOMICS, EXPRESSION, MEMBRANE
- in
- Electrophoresis
- volume
- 22
- issue
- 18
- pages
- 3984 - 3992
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000171863900019
- scopus:0034761614
- ISSN
- 0173-0835
- DOI
- 10.1002/1522-2683(200110)22:18<3984::aid-elps3984>3.0.co;2-t
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Biomedical Engineering (011200011)
- id
- 981ca33f-b55e-46fc-88d9-6d12e3d557ca (old id 2376314)
- date added to LUP
- 2016-04-01 11:49:09
- date last changed
- 2022-01-26 18:41:20
@article{981ca33f-b55e-46fc-88d9-6d12e3d557ca, abstract = {{A novel disposable high-density matrix assisted laser desorption/ionization (MALDI) target plate made either from polymethylmethacrylate (PMMA) or polycarbonate (PC) is presented where thousands (1200-1600) of samples can be deposited and subsequently, analyzed by MALDI-time of flight (TOF) mass spectrometry. Good reproducibility, was obtained across the plate regardless of position on the target plate with a relative standard deviation (RSD) on the peak intensity of typically 30% calculated from data generated by analysis of a 10 nM peptide mixture of angiotensin. I, II, III and bradykinin. The nanovial array format combined with microdispensing technology makes It possible to carry out in-vial, chemistry on deposited samples. This is demonstrated by the analysis of peptides from beta -casein and subsequent in-vial dephosphorylation of its phosphopeptides at 10 fmol levels by microdispensing of alkaline phosphatase, into the nanovial. The mass spectra obtained from these polymeric targets provides can also be used in high sensitivity applications as shown by peptide mass fingerprinting of human fibroblast proteins separated by two-dimensional gel, electrophoresis.}}, author = {{Ekstrom, S. and Nilsson, J. and Helldin, G. and Laurell, Thomas and Marko-Varga, György}}, issn = {{0173-0835}}, keywords = {{polymer high-density matrix assisted laser desorption; ionization target; plates; phosphoproteins; peptide mapping; array; microdispensing; IMMOBILIZED PH GRADIENTS; PROTEIN IDENTIFICATION; SAMPLE PREPARATION; 2-DIMENSIONAL ELECTROPHORESIS; LIQUID-CHROMATOGRAPHY; SEQUENCE; DATABASES; PURIFICATION; PROTEOMICS; EXPRESSION; MEMBRANE}}, language = {{eng}}, number = {{18}}, pages = {{3984--3992}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Electrophoresis}}, title = {{Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: II. Biological applications}}, url = {{http://dx.doi.org/10.1002/1522-2683(200110)22:18<3984::aid-elps3984>3.0.co;2-t}}, doi = {{10.1002/1522-2683(200110)22:18<3984::aid-elps3984>3.0.co;2-t}}, volume = {{22}}, year = {{2001}}, }