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Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: II. Biological applications

Ekstrom, S.; Nilsson, J.; Helldin, G.; Laurell, Thomas LU and Marko-Varga, György LU (2001) In Electrophoresis 22(18). p.3984-3992
Abstract
A novel disposable high-density matrix assisted laser desorption/ionization (MALDI) target plate made either from polymethylmethacrylate (PMMA) or polycarbonate (PC) is presented where thousands (1200-1600) of samples can be deposited and subsequently, analyzed by MALDI-time of flight (TOF) mass spectrometry. Good reproducibility, was obtained across the plate regardless of position on the target plate with a relative standard deviation (RSD) on the peak intensity of typically 30% calculated from data generated by analysis of a 10 nM peptide mixture of angiotensin. I, II, III and bradykinin. The nanovial array format combined with microdispensing technology makes It possible to carry out in-vial, chemistry on deposited samples. This is... (More)
A novel disposable high-density matrix assisted laser desorption/ionization (MALDI) target plate made either from polymethylmethacrylate (PMMA) or polycarbonate (PC) is presented where thousands (1200-1600) of samples can be deposited and subsequently, analyzed by MALDI-time of flight (TOF) mass spectrometry. Good reproducibility, was obtained across the plate regardless of position on the target plate with a relative standard deviation (RSD) on the peak intensity of typically 30% calculated from data generated by analysis of a 10 nM peptide mixture of angiotensin. I, II, III and bradykinin. The nanovial array format combined with microdispensing technology makes It possible to carry out in-vial, chemistry on deposited samples. This is demonstrated by the analysis of peptides from beta -casein and subsequent in-vial dephosphorylation of its phosphopeptides at 10 fmol levels by microdispensing of alkaline phosphatase, into the nanovial. The mass spectra obtained from these polymeric targets provides can also be used in high sensitivity applications as shown by peptide mass fingerprinting of human fibroblast proteins separated by two-dimensional gel, electrophoresis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polymer high-density matrix assisted laser desorption, ionization target, plates, phosphoproteins, peptide mapping, array, microdispensing, IMMOBILIZED PH GRADIENTS, PROTEIN IDENTIFICATION, SAMPLE PREPARATION, 2-DIMENSIONAL ELECTROPHORESIS, LIQUID-CHROMATOGRAPHY, SEQUENCE, DATABASES, PURIFICATION, PROTEOMICS, EXPRESSION, MEMBRANE
in
Electrophoresis
volume
22
issue
18
pages
3984 - 3992
publisher
John Wiley & Sons
external identifiers
  • wos:000171863900019
  • scopus:0034761614
ISSN
0173-0835
DOI
10.1002/1522-2683(200110)22:18<3984::aid-elps3984>3.0.co;2-t
language
English
LU publication?
yes
id
981ca33f-b55e-46fc-88d9-6d12e3d557ca (old id 2376314)
date added to LUP
2012-03-23 09:41:45
date last changed
2018-01-07 05:25:13
@article{981ca33f-b55e-46fc-88d9-6d12e3d557ca,
  abstract     = {A novel disposable high-density matrix assisted laser desorption/ionization (MALDI) target plate made either from polymethylmethacrylate (PMMA) or polycarbonate (PC) is presented where thousands (1200-1600) of samples can be deposited and subsequently, analyzed by MALDI-time of flight (TOF) mass spectrometry. Good reproducibility, was obtained across the plate regardless of position on the target plate with a relative standard deviation (RSD) on the peak intensity of typically 30% calculated from data generated by analysis of a 10 nM peptide mixture of angiotensin. I, II, III and bradykinin. The nanovial array format combined with microdispensing technology makes It possible to carry out in-vial, chemistry on deposited samples. This is demonstrated by the analysis of peptides from beta -casein and subsequent in-vial dephosphorylation of its phosphopeptides at 10 fmol levels by microdispensing of alkaline phosphatase, into the nanovial. The mass spectra obtained from these polymeric targets provides can also be used in high sensitivity applications as shown by peptide mass fingerprinting of human fibroblast proteins separated by two-dimensional gel, electrophoresis.},
  author       = {Ekstrom, S. and Nilsson, J. and Helldin, G. and Laurell, Thomas and Marko-Varga, György},
  issn         = {0173-0835},
  keyword      = {polymer high-density matrix assisted laser desorption,ionization target,plates,phosphoproteins,peptide mapping,array,microdispensing,IMMOBILIZED PH GRADIENTS,PROTEIN IDENTIFICATION,SAMPLE PREPARATION,2-DIMENSIONAL ELECTROPHORESIS,LIQUID-CHROMATOGRAPHY,SEQUENCE,DATABASES,PURIFICATION,PROTEOMICS,EXPRESSION,MEMBRANE},
  language     = {eng},
  number       = {18},
  pages        = {3984--3992},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: II. Biological applications},
  url          = {http://dx.doi.org/10.1002/1522-2683(200110)22:18<3984::aid-elps3984>3.0.co;2-t},
  volume       = {22},
  year         = {2001},
}