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Silicon microstructures for high-speed and high-sensitivity protein identifications

Laurell, Thomas LU ; Nilsson, J. and Marko-Varga, György LU (2001) In Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences 752(2). p.217-232
Abstract
Silicon microtechnology has been used to develop a microstructure toolbox in order to enable high accuracy protein identification. During the last 2 years we developed and applied monocrystalline silicon structures and established new automated protein analysis platforms, The development of a high throughput protein platform is presented where fully automated protein identifications are performed. It includes the reduction and alkylation of the protein sample in a standard 96- or 384-well plate format prior to injection of 1 mul samples into the continuous flow based microtechnology platform. The processed sample is transferred to a microchip nanovial array target using piezoelectric microdispensing. identification is made by MALDI-TOF MS... (More)
Silicon microtechnology has been used to develop a microstructure toolbox in order to enable high accuracy protein identification. During the last 2 years we developed and applied monocrystalline silicon structures and established new automated protein analysis platforms, The development of a high throughput protein platform is presented where fully automated protein identifications are performed. It includes the reduction and alkylation of the protein sample in a standard 96- or 384-well plate format prior to injection of 1 mul samples into the continuous flow based microtechnology platform. The processed sample is transferred to a microchip nanovial array target using piezoelectric microdispensing. identification is made by MALDI-TOF MS and a database search. After the initial sample reduction and alkylation period of 50 min the platform can digest and process protein samples at a speed of 100 samples in 210 min. An optional configuration of the platform, operating the dispenser in the 'static mode', enables on-target enrichment of low abundant proteins and peptides e.g. from 2DE samples. This makes detection at the low attomole level possible. (C) 2001 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
silicon microstructures, proteins, FLIGHT MASS-SPECTROMETRY, FLOW-THROUGH DISPENSER, SAMPLE PREPARATION, POROUS SILICON, MATRIX, MALDI
in
Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
volume
752
issue
2
pages
217 - 232
publisher
Elsevier
external identifiers
  • wos:000167347000004
  • scopus:0035836056
ISSN
0378-4347
DOI
10.1016/s0378-4347(00)00358-3
language
English
LU publication?
yes
id
5af31fe7-2756-482d-aef2-f62f56de6e49 (old id 2376386)
date added to LUP
2012-03-23 09:17:23
date last changed
2018-05-29 12:00:38
@article{5af31fe7-2756-482d-aef2-f62f56de6e49,
  abstract     = {Silicon microtechnology has been used to develop a microstructure toolbox in order to enable high accuracy protein identification. During the last 2 years we developed and applied monocrystalline silicon structures and established new automated protein analysis platforms, The development of a high throughput protein platform is presented where fully automated protein identifications are performed. It includes the reduction and alkylation of the protein sample in a standard 96- or 384-well plate format prior to injection of 1 mul samples into the continuous flow based microtechnology platform. The processed sample is transferred to a microchip nanovial array target using piezoelectric microdispensing. identification is made by MALDI-TOF MS and a database search. After the initial sample reduction and alkylation period of 50 min the platform can digest and process protein samples at a speed of 100 samples in 210 min. An optional configuration of the platform, operating the dispenser in the 'static mode', enables on-target enrichment of low abundant proteins and peptides e.g. from 2DE samples. This makes detection at the low attomole level possible. (C) 2001 Elsevier Science B.V. All rights reserved.},
  author       = {Laurell, Thomas and Nilsson, J. and Marko-Varga, György},
  issn         = {0378-4347},
  keyword      = {silicon microstructures,proteins,FLIGHT MASS-SPECTROMETRY,FLOW-THROUGH DISPENSER,SAMPLE PREPARATION,POROUS SILICON,MATRIX,MALDI},
  language     = {eng},
  number       = {2},
  pages        = {217--232},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences},
  title        = {Silicon microstructures for high-speed and high-sensitivity protein identifications},
  url          = {http://dx.doi.org/10.1016/s0378-4347(00)00358-3},
  volume       = {752},
  year         = {2001},
}