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Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: I. Microstructure development and manufacturing

Marko-Varga, György LU ; Ekstrom, S. ; Helldin, G. ; Nilsson, J. and Laurell, Thomas LU (2001) In Electrophoresis 22(18). p.3978-3983
Abstract
In order to meet the expected enormous demand for mass spectrometry (MS)through-, put as a result of the current efforts to completely map the human proteome, this paper presents a new concept for low-cost high-throughput protein identification by matrix assisted laser desorption/ionization-time of flight-(MALDI-TOF)-MS peptide mapping using disposable polymeric high-density nanovial MALDI target plates. By means of microfabrication technology precision engineered nanovial arrays are fabricated in polymer substrates such as polymethylmethacrylate (PMMA) and polycarbonate (PC). The target plate fabrication processes investigated were precision. micromilling, cold embossing and injection moulding (work in progress). Nanovial dimensions were... (More)
In order to meet the expected enormous demand for mass spectrometry (MS)through-, put as a result of the current efforts to completely map the human proteome, this paper presents a new concept for low-cost high-throughput protein identification by matrix assisted laser desorption/ionization-time of flight-(MALDI-TOF)-MS peptide mapping using disposable polymeric high-density nanovial MALDI target plates. By means of microfabrication technology precision engineered nanovial arrays are fabricated in polymer substrates such as polymethylmethacrylate (PMMA) and polycarbonate (PC). The target plate fabrication processes investigated were precision. micromilling, cold embossing and injection moulding (work in progress). Nanovial dimensions were 300, 400 or 500 mum. Typical array densities were 165 nanovials/cm(2), which corresponds to 3300 vials on a full Applied Biosystems MALDI target plate. Obtained MALDI data displayed equal mass resolution, accuracy, signal intensity for peptide standards as compared to high-density silicon nanovial arrays previously reported by our group [7], as well as conventional stainless steel or gold targets. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polymeric high-density matrix-assisted laser desorption, ionization, target plates, nanovial, microfabrication, peptides, array
in
Electrophoresis
volume
22
issue
18
pages
3978 - 3983
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000171863900018
  • scopus:0034755602
ISSN
0173-0835
DOI
10.1002/1522-2683(200110)22:18<3978::aid-elps3978>3.0.co;2-7
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Biomedical Engineering (011200011)
id
3f77fd42-de35-4163-ae42-3b4ec203a74d (old id 2376392)
date added to LUP
2016-04-01 12:14:41
date last changed
2025-01-02 10:54:37
@article{3f77fd42-de35-4163-ae42-3b4ec203a74d,
  abstract     = {{In order to meet the expected enormous demand for mass spectrometry (MS)through-, put as a result of the current efforts to completely map the human proteome, this paper presents a new concept for low-cost high-throughput protein identification by matrix assisted laser desorption/ionization-time of flight-(MALDI-TOF)-MS peptide mapping using disposable polymeric high-density nanovial MALDI target plates. By means of microfabrication technology precision engineered nanovial arrays are fabricated in polymer substrates such as polymethylmethacrylate (PMMA) and polycarbonate (PC). The target plate fabrication processes investigated were precision. micromilling, cold embossing and injection moulding (work in progress). Nanovial dimensions were 300, 400 or 500 mum. Typical array densities were 165 nanovials/cm(2), which corresponds to 3300 vials on a full Applied Biosystems MALDI target plate. Obtained MALDI data displayed equal mass resolution, accuracy, signal intensity for peptide standards as compared to high-density silicon nanovial arrays previously reported by our group [7], as well as conventional stainless steel or gold targets.}},
  author       = {{Marko-Varga, György and Ekstrom, S. and Helldin, G. and Nilsson, J. and Laurell, Thomas}},
  issn         = {{0173-0835}},
  keywords     = {{polymeric high-density matrix-assisted laser desorption; ionization; target plates; nanovial; microfabrication; peptides; array}},
  language     = {{eng}},
  number       = {{18}},
  pages        = {{3978--3983}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Electrophoresis}},
  title        = {{Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: I. Microstructure development and manufacturing}},
  url          = {{http://dx.doi.org/10.1002/1522-2683(200110)22:18<3978::aid-elps3978>3.0.co;2-7}},
  doi          = {{10.1002/1522-2683(200110)22:18<3978::aid-elps3978>3.0.co;2-7}},
  volume       = {{22}},
  year         = {{2001}},
}