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In vivo measurements of diffuse reflectance and time-resolved autofluorescence emission spectra of basal cell carcinomas

Thompson, Alex J.; Coda, Sergio; Brydegaard, Mikkel LU ; Kennedy, Gordon; Patalay, Rakesh; Waitong-Bramming, Ulrika; De Beule, Pieter A. A.; Neil, Mark A. A.; Andersson-Engels, Stefan LU and Bendsöe, Niels LU , et al. (2012) In Journal of Biophotonics 5(3). p.240-254
Abstract
We present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This... (More)
We present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This approach was able to correctly diagnose 87% of the BCCs. With 445 nm excitation a decrease in the spectrally averaged fluorescence lifetime was observed between normal tissue and BCC lesions with a mean value of 886 ps. Furthermore, the fluorescence lifetime for BCCs was lower than that of the surrounding healthy tissue in all cases and statistical analysis of the data revealed that this decrease was significant (p = 0.002). (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Less)
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Contribution to journal
publication status
published
subject
keywords
fluorescence, fluorescence lifetime, diffuse reflectance, skin cancer, spectrometer, fibre probe
in
Journal of Biophotonics
volume
5
issue
3
pages
240 - 254
publisher
John Wiley & Sons
external identifiers
  • wos:000301053900008
  • scopus:84857976890
ISSN
1864-063X
DOI
10.1002/jbio.201100126
language
English
LU publication?
yes
id
82fc79f5-a950-40ed-819b-745d3ba6a063 (old id 2379177)
date added to LUP
2012-03-27 23:08:57
date last changed
2017-11-19 03:17:59
@misc{82fc79f5-a950-40ed-819b-745d3ba6a063,
  abstract     = {We present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This approach was able to correctly diagnose 87% of the BCCs. With 445 nm excitation a decrease in the spectrally averaged fluorescence lifetime was observed between normal tissue and BCC lesions with a mean value of 886 ps. Furthermore, the fluorescence lifetime for BCCs was lower than that of the surrounding healthy tissue in all cases and statistical analysis of the data revealed that this decrease was significant (p = 0.002). (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)},
  author       = {Thompson, Alex J. and Coda, Sergio and Brydegaard, Mikkel and Kennedy, Gordon and Patalay, Rakesh and Waitong-Bramming, Ulrika and De Beule, Pieter A. A. and Neil, Mark A. A. and Andersson-Engels, Stefan and Bendsöe, Niels and French, Paul M. W. and Svanberg, Katarina and Dunsby, Chris},
  issn         = {1864-063X},
  keyword      = {fluorescence,fluorescence lifetime,diffuse reflectance,skin cancer,spectrometer,fibre probe},
  language     = {eng},
  number       = {3},
  pages        = {240--254},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Biophotonics},
  title        = {In vivo measurements of diffuse reflectance and time-resolved autofluorescence emission spectra of basal cell carcinomas},
  url          = {http://dx.doi.org/10.1002/jbio.201100126},
  volume       = {5},
  year         = {2012},
}