Sensory nerve inactivation by resiniferatoxin improves insulin sensitivity in male obese Zucker rats
(2005) In American Journal of Physiology: Endocrinology and Metabolism 288(6). p.1137-1145- Abstract
- Recent studies have suggested that sensory nerves may influence insulin secretion and action. The present study investigated the effects of resiniferatoxin (RTX) inactivation of sensory nerves ( desensitization) on oral glucose tolerance, insulin secretion and whole body insulin sensitivity in the glucose intolerant, hyperinsulinemic, and insulin-resistant obese Zucker rat. After RTX treatment (0.05 mg/kg RTX sc given at ages 8, 10, and 12 wk), fasting plasma insulin was reduced ( P < 0.0005), and oral glucose tolerance was improved ( P < 0.005). Pancreas perfusion showed that baseline insulin secretion ( 7 mM glucose) was lower in RTX-treated rats ( P = 0.01). Insulin secretory responsiveness to 20 mM glucose was enhanced in the... (More)
- Recent studies have suggested that sensory nerves may influence insulin secretion and action. The present study investigated the effects of resiniferatoxin (RTX) inactivation of sensory nerves ( desensitization) on oral glucose tolerance, insulin secretion and whole body insulin sensitivity in the glucose intolerant, hyperinsulinemic, and insulin-resistant obese Zucker rat. After RTX treatment (0.05 mg/kg RTX sc given at ages 8, 10, and 12 wk), fasting plasma insulin was reduced ( P < 0.0005), and oral glucose tolerance was improved ( P < 0.005). Pancreas perfusion showed that baseline insulin secretion ( 7 mM glucose) was lower in RTX-treated rats ( P = 0.01). Insulin secretory responsiveness to 20 mM glucose was enhanced in the perfused pancreas of RTX-treated rats ( P < 0.005) but unaffected in stimulated, isolated pancreatic islets. At the peak of spontaneous insulin resistance in the obese Zucker rat, insulin sensitivity was substantially improved after RTX treatment, as evidenced by higher glucose infusion rates (GIR) required to maintain euglycemia during a hyperinsulinemic euglycemic ( 5 mU center dot kg(-1) center dot min(-1)) clamp (GIR(60 - 120min): 5.97 +/- 0.62 vs. 11.65 +/- 0.83 mg center dot kg(-1) center dot min(-1) in RTX-treated rats, P = 0.003). In conclusion, RTX treatment and, hence, sensory nerve desensitization of adult male obese Zucker rats improved oral glucose tolerance by enhancing insulin secretion, and, in particular, by improving insulin sensitivity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/239196
- author
- Moesgaard, S G ; Brand, C L ; Sturis, J ; Ahrén, Bo LU ; Wilken, M ; Fleckner, J ; Carr, R D ; Svendsen, O ; Hansen, A J and Gram, D X
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- capsaicin, hyperinsulinemic euglycemic clamp, obesity
- in
- American Journal of Physiology: Endocrinology and Metabolism
- volume
- 288
- issue
- 6
- pages
- 1137 - 1145
- publisher
- American Physiological Society
- external identifiers
-
- wos:000229308000011
- pmid:15883192
- scopus:21144438021
- ISSN
- 1522-1555
- DOI
- 10.1152/ajpendo.00356.2004
- language
- English
- LU publication?
- yes
- id
- bed33aab-3acc-4b7b-85d2-7c97c6c4ab48 (old id 239196)
- date added to LUP
- 2016-04-01 17:01:53
- date last changed
- 2024-02-27 03:25:17
@article{bed33aab-3acc-4b7b-85d2-7c97c6c4ab48,
abstract = {{Recent studies have suggested that sensory nerves may influence insulin secretion and action. The present study investigated the effects of resiniferatoxin (RTX) inactivation of sensory nerves ( desensitization) on oral glucose tolerance, insulin secretion and whole body insulin sensitivity in the glucose intolerant, hyperinsulinemic, and insulin-resistant obese Zucker rat. After RTX treatment (0.05 mg/kg RTX sc given at ages 8, 10, and 12 wk), fasting plasma insulin was reduced ( P < 0.0005), and oral glucose tolerance was improved ( P < 0.005). Pancreas perfusion showed that baseline insulin secretion ( 7 mM glucose) was lower in RTX-treated rats ( P = 0.01). Insulin secretory responsiveness to 20 mM glucose was enhanced in the perfused pancreas of RTX-treated rats ( P < 0.005) but unaffected in stimulated, isolated pancreatic islets. At the peak of spontaneous insulin resistance in the obese Zucker rat, insulin sensitivity was substantially improved after RTX treatment, as evidenced by higher glucose infusion rates (GIR) required to maintain euglycemia during a hyperinsulinemic euglycemic ( 5 mU center dot kg(-1) center dot min(-1)) clamp (GIR(60 - 120min): 5.97 +/- 0.62 vs. 11.65 +/- 0.83 mg center dot kg(-1) center dot min(-1) in RTX-treated rats, P = 0.003). In conclusion, RTX treatment and, hence, sensory nerve desensitization of adult male obese Zucker rats improved oral glucose tolerance by enhancing insulin secretion, and, in particular, by improving insulin sensitivity.}},
author = {{Moesgaard, S G and Brand, C L and Sturis, J and Ahrén, Bo and Wilken, M and Fleckner, J and Carr, R D and Svendsen, O and Hansen, A J and Gram, D X}},
issn = {{1522-1555}},
keywords = {{capsaicin; hyperinsulinemic euglycemic clamp; obesity}},
language = {{eng}},
number = {{6}},
pages = {{1137--1145}},
publisher = {{American Physiological Society}},
series = {{American Journal of Physiology: Endocrinology and Metabolism}},
title = {{Sensory nerve inactivation by resiniferatoxin improves insulin sensitivity in male obese Zucker rats}},
url = {{http://dx.doi.org/10.1152/ajpendo.00356.2004}},
doi = {{10.1152/ajpendo.00356.2004}},
volume = {{288}},
year = {{2005}},
}