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Dynamic arraying of microbeads for bioassays in microfluidic channels

Lilliehorn, T; Evander, Mikael LU ; Simu, U; Johansson, S; Almqvist, Monica LU ; Nilsson, Johan LU and Laurell, Thomas LU (2005) In Sensors and Actuators B: Chemical 106(2). p.851-858
Abstract
This paper proposes a new dynamic mode of generating bioanalytical arrays in microfluidic systems, based on ultrasonic trapping of microbeads using acoustic forces in standing waves. Trapping of microbead clusters in an array format within a flow-through device is demonstrated for the first time using a device with three integrated ultrasonic microtransducers. The lateral extension of each trapping site was essentially determined by the corresponding microtransducer dimensions, 0.8 mm x 0.8 mm. The flow-through volume was approximately 1 μ l and the trapping site volumes about 100 nl each. The strength of trapping was investigated, showing that 50% of the initially trapped beads were still trapped at a perfusion rate of 10 μ l/min. A... (More)
This paper proposes a new dynamic mode of generating bioanalytical arrays in microfluidic systems, based on ultrasonic trapping of microbeads using acoustic forces in standing waves. Trapping of microbead clusters in an array format within a flow-through device is demonstrated for the first time using a device with three integrated ultrasonic microtransducers. The lateral extension of each trapping site was essentially determined by the corresponding microtransducer dimensions, 0.8 mm x 0.8 mm. The flow-through volume was approximately 1 μ l and the trapping site volumes about 100 nl each. The strength of trapping was investigated, showing that 50% of the initially trapped beads were still trapped at a perfusion rate of 10 μ l/min. A fluorescence based avidin bioassay was successfully performed on biotin-coated microbeads trapped in the flow-through device, providing a first proof of principle of the proposed dynamic arraying concept. The dynamic arraying is believed to be expandable to two dimensions, thus, with a prospect of performing targeted and highly parallel protein analysis in microfluidic devices. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ultrasound, trapping, bead, proteomics, array, microchannel
in
Sensors and Actuators B: Chemical
volume
106
issue
2
pages
851 - 858
publisher
Elsevier
external identifiers
  • wos:000228969100051
  • scopus:17644423133
ISSN
0925-4005
DOI
10.1016/j.snb.2004.07.003
language
English
LU publication?
yes
id
a5b5e0cc-86d3-48ca-bf54-7faa9a297188 (old id 240435)
date added to LUP
2007-08-09 15:47:45
date last changed
2017-01-01 07:02:11
@article{a5b5e0cc-86d3-48ca-bf54-7faa9a297188,
  abstract     = {This paper proposes a new dynamic mode of generating bioanalytical arrays in microfluidic systems, based on ultrasonic trapping of microbeads using acoustic forces in standing waves. Trapping of microbead clusters in an array format within a flow-through device is demonstrated for the first time using a device with three integrated ultrasonic microtransducers. The lateral extension of each trapping site was essentially determined by the corresponding microtransducer dimensions, 0.8 mm x 0.8 mm. The flow-through volume was approximately 1 μ l and the trapping site volumes about 100 nl each. The strength of trapping was investigated, showing that 50% of the initially trapped beads were still trapped at a perfusion rate of 10 μ l/min. A fluorescence based avidin bioassay was successfully performed on biotin-coated microbeads trapped in the flow-through device, providing a first proof of principle of the proposed dynamic arraying concept. The dynamic arraying is believed to be expandable to two dimensions, thus, with a prospect of performing targeted and highly parallel protein analysis in microfluidic devices.},
  author       = {Lilliehorn, T and Evander, Mikael and Simu, U and Johansson, S and Almqvist, Monica and Nilsson, Johan and Laurell, Thomas},
  issn         = {0925-4005},
  keyword      = {ultrasound,trapping,bead,proteomics,array,microchannel},
  language     = {eng},
  number       = {2},
  pages        = {851--858},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Dynamic arraying of microbeads for bioassays in microfluidic channels},
  url          = {http://dx.doi.org/10.1016/j.snb.2004.07.003},
  volume       = {106},
  year         = {2005},
}