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Sampling of urinary cadmium: differences between 24-h urine and overnight spot urine sampling, and impact of adjustment for dilution

Akerstrom, Magnus; Lundh, Thomas LU ; Barregard, Lars and Sallsten, Gerd (2012) In International Archives of Occupational and Environmental Health 85(2). p.189-196
Abstract
Urinary cadmium (U-Cd) sampling can be done either by 24-h urine or spot urine sampling, and adjustment for dilution is usually needed. The choice of sampling period and adjustment technique could, however, potentially induce bias. The aim of the study was to compare 24-h urine and spot urine sampling and two dilution adjustment techniques, when assessing U-Cd. Separate 24-h urine (U24) and timed overnight spot urine (UON) samples were collected from 152 healthy kidney donors. U-Cd, creatinine concentration (U-Crea) and specific gravity (SG) were analysed. Differences between U24 and UON samples were tested using paired t test, and the effect of urinary flow rate (UF) was assessed by linear regression. The cadmium excretion rate (U-Cd/h)... (More)
Urinary cadmium (U-Cd) sampling can be done either by 24-h urine or spot urine sampling, and adjustment for dilution is usually needed. The choice of sampling period and adjustment technique could, however, potentially induce bias. The aim of the study was to compare 24-h urine and spot urine sampling and two dilution adjustment techniques, when assessing U-Cd. Separate 24-h urine (U24) and timed overnight spot urine (UON) samples were collected from 152 healthy kidney donors. U-Cd, creatinine concentration (U-Crea) and specific gravity (SG) were analysed. Differences between U24 and UON samples were tested using paired t test, and the effect of urinary flow rate (UF) was assessed by linear regression. The cadmium excretion rate (U-Cd/h) was lower in the UON than in U24 samples (mean 0.017 mu g/h vs. 0.021 mu g/h; p < 0.001). This decrease was found also for the creatinine-adjusted U-Cd (U-CdCrea) (mean 0.36 mu g/gC and 0.41 mu g/gC; p < 0.001). For U-Cd adjusted for specific gravity (U-CdSG), the difference was reversed, but not statistically significant. The creatinine excretion rate (U-Crea/h) decreased at low UF, especially in the UON. Since U-Cd/h was lower in UON than in U24 samples, the former will underestimate the true Cd excretion. This was seen for U-CdCrea but not for U-CdSG. However, it may be an advantage that the U-CdSG is similar, irrespective of sampling strategy. At low UF, U-CdCrea will be biased upwards. Whether U24 or UON samples adjusted for U-Crea or SG best reflect kidney-Cd is still unknown. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Urinary cadmium, Creatinine, Specific gravity, 24-h urine, Spot urine
in
International Archives of Occupational and Environmental Health
volume
85
issue
2
pages
189 - 196
publisher
Springer
external identifiers
  • wos:000299523300008
  • scopus:84857503587
ISSN
1432-1246
DOI
10.1007/s00420-011-0658-z
language
English
LU publication?
yes
id
d3202594-b82a-4d52-829b-f88c91c6adfd (old id 2416154)
date added to LUP
2012-04-02 09:38:21
date last changed
2017-01-01 06:18:02
@article{d3202594-b82a-4d52-829b-f88c91c6adfd,
  abstract     = {Urinary cadmium (U-Cd) sampling can be done either by 24-h urine or spot urine sampling, and adjustment for dilution is usually needed. The choice of sampling period and adjustment technique could, however, potentially induce bias. The aim of the study was to compare 24-h urine and spot urine sampling and two dilution adjustment techniques, when assessing U-Cd. Separate 24-h urine (U24) and timed overnight spot urine (UON) samples were collected from 152 healthy kidney donors. U-Cd, creatinine concentration (U-Crea) and specific gravity (SG) were analysed. Differences between U24 and UON samples were tested using paired t test, and the effect of urinary flow rate (UF) was assessed by linear regression. The cadmium excretion rate (U-Cd/h) was lower in the UON than in U24 samples (mean 0.017 mu g/h vs. 0.021 mu g/h; p &lt; 0.001). This decrease was found also for the creatinine-adjusted U-Cd (U-CdCrea) (mean 0.36 mu g/gC and 0.41 mu g/gC; p &lt; 0.001). For U-Cd adjusted for specific gravity (U-CdSG), the difference was reversed, but not statistically significant. The creatinine excretion rate (U-Crea/h) decreased at low UF, especially in the UON. Since U-Cd/h was lower in UON than in U24 samples, the former will underestimate the true Cd excretion. This was seen for U-CdCrea but not for U-CdSG. However, it may be an advantage that the U-CdSG is similar, irrespective of sampling strategy. At low UF, U-CdCrea will be biased upwards. Whether U24 or UON samples adjusted for U-Crea or SG best reflect kidney-Cd is still unknown.},
  author       = {Akerstrom, Magnus and Lundh, Thomas and Barregard, Lars and Sallsten, Gerd},
  issn         = {1432-1246},
  keyword      = {Urinary cadmium,Creatinine,Specific gravity,24-h urine,Spot urine},
  language     = {eng},
  number       = {2},
  pages        = {189--196},
  publisher    = {Springer},
  series       = {International Archives of Occupational and Environmental Health},
  title        = {Sampling of urinary cadmium: differences between 24-h urine and overnight spot urine sampling, and impact of adjustment for dilution},
  url          = {http://dx.doi.org/10.1007/s00420-011-0658-z},
  volume       = {85},
  year         = {2012},
}