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Placenta-derived extracellular vesicles : Their cargo and possible functions

Familari, Mary LU ; Cronqvist, Tina LU ; Masoumi, Zahra LU and Hansson, Stefan LU (2017) In Reproduction, Fertility and Development 29(3). p.433-447
Abstract

The literature on extracellular vesicles consists of rapidly expanding and often contradictory information. In this paper we attempt to review what is currently known regarding extracellular vesicles released specifically from human placental syncytiotrophoblast cells with a focus on the common but complex pregnancy-associated syndrome pre-eclampsia, where the level of syncytiotrophoblast extracellular vesicle release is significantly increased. We review common methods for syncytiotrophoblast extracellular vesicle derivation and isolation and we discuss the cargo of syncytiotrophoblast extracellular vesicles including proteins, RNA and lipids and their possible functions. A meta-analysis of available trophoblast-derived extracellular... (More)

The literature on extracellular vesicles consists of rapidly expanding and often contradictory information. In this paper we attempt to review what is currently known regarding extracellular vesicles released specifically from human placental syncytiotrophoblast cells with a focus on the common but complex pregnancy-associated syndrome pre-eclampsia, where the level of syncytiotrophoblast extracellular vesicle release is significantly increased. We review common methods for syncytiotrophoblast extracellular vesicle derivation and isolation and we discuss the cargo of syncytiotrophoblast extracellular vesicles including proteins, RNA and lipids and their possible functions. A meta-analysis of available trophoblast-derived extracellular vesicle proteomic datasets revealed only three proteins in common: albumin, fibronectin-1 and plasminogen activator inhibitor-1, suggesting some variability in vesicle cargo, most likely reflecting stage and cell type of origin. We discuss the possible sources of variability that may have led to the low number of common markers, which has led us to speculate that markers and density in common use may not be strict criteria for identifying and isolating placenta-derived exosomes.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
endothelial cells, exosomes, lipidomics, meta-analysis, proteomics, syncytiotrophoblast cells, transcriptome, trophoblast cells
in
Reproduction, Fertility and Development
volume
29
issue
3
pages
15 pages
publisher
CSIRO
external identifiers
  • scopus:85013498078
  • wos:000395555500001
ISSN
1031-3613
DOI
10.1071/RD15143
language
English
LU publication?
yes
id
2423f782-7407-483a-b3d6-f6b85168d7a1
date added to LUP
2017-03-24 15:19:27
date last changed
2018-08-26 04:53:38
@article{2423f782-7407-483a-b3d6-f6b85168d7a1,
  abstract     = {<p>The literature on extracellular vesicles consists of rapidly expanding and often contradictory information. In this paper we attempt to review what is currently known regarding extracellular vesicles released specifically from human placental syncytiotrophoblast cells with a focus on the common but complex pregnancy-associated syndrome pre-eclampsia, where the level of syncytiotrophoblast extracellular vesicle release is significantly increased. We review common methods for syncytiotrophoblast extracellular vesicle derivation and isolation and we discuss the cargo of syncytiotrophoblast extracellular vesicles including proteins, RNA and lipids and their possible functions. A meta-analysis of available trophoblast-derived extracellular vesicle proteomic datasets revealed only three proteins in common: albumin, fibronectin-1 and plasminogen activator inhibitor-1, suggesting some variability in vesicle cargo, most likely reflecting stage and cell type of origin. We discuss the possible sources of variability that may have led to the low number of common markers, which has led us to speculate that markers and density in common use may not be strict criteria for identifying and isolating placenta-derived exosomes.</p>},
  author       = {Familari, Mary and Cronqvist, Tina and Masoumi, Zahra and Hansson, Stefan},
  issn         = {1031-3613},
  keyword      = {endothelial cells,exosomes,lipidomics,meta-analysis,proteomics,syncytiotrophoblast cells,transcriptome,trophoblast cells},
  language     = {eng},
  number       = {3},
  pages        = {433--447},
  publisher    = {CSIRO},
  series       = {Reproduction, Fertility and Development},
  title        = {Placenta-derived extracellular vesicles : Their cargo and possible functions},
  url          = {http://dx.doi.org/10.1071/RD15143},
  volume       = {29},
  year         = {2017},
}