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Expression of Activating Transcription Factor 3 (ATF 3) and caspase 3 in Schwann cells and axonal outgrowth after sciatic nerve repair in diabetic BB rats.

Stenberg, Lena LU ; Kanje, Martin; Dolezal, Katarina and Dahlin, Lars LU (2012) In Neuroscience Letters 515(1). p.34-38
Abstract
The aim of this study was to evaluate nerve regeneration in relation to the transcription factor, Activating Transcription Factor 3 (ATF 3), and an apoptotic marker, caspase 3, in the Schwann cells of diabetic BB rats (i.e. display type 1 diabetes phenotype). Sciatic nerves in healthy Wistar rats and in diabetic BB rats were transected and immediately repaired. Axonal outgrowth (neurofilament staining) and expression of ATF 3 and caspase 3 were quantified by immunohistochemistry after six days. There was no difference in axonal outgrowth between healthy and diabetic rats. However, the sciatic nerve in the diabetic rats exhibited a larger number of ATF 3 expressing Schwann cells at the site of the lesion and also a higher number of caspase... (More)
The aim of this study was to evaluate nerve regeneration in relation to the transcription factor, Activating Transcription Factor 3 (ATF 3), and an apoptotic marker, caspase 3, in the Schwann cells of diabetic BB rats (i.e. display type 1 diabetes phenotype). Sciatic nerves in healthy Wistar rats and in diabetic BB rats were transected and immediately repaired. Axonal outgrowth (neurofilament staining) and expression of ATF 3 and caspase 3 were quantified by immunohistochemistry after six days. There was no difference in axonal outgrowth between healthy and diabetic rats. However, the sciatic nerve in the diabetic rats exhibited a larger number of ATF 3 expressing Schwann cells at the site of the lesion and also a higher number of caspase 3 expressing Schwann cells. Similar differences were observed in the distal nerve segment between the healthy and diabetic rats. There were no correlations between the number of Schwann cells expressing ATF 3 and caspase 3. Thus, diabetic BB rats display an increased activation of ATF 3 and also a rise in apoptotic caspase 3 expressing Schwann cells, but with no discrepancy in length of axonal outgrowth after nerve injury and repair at six days. Knowledge about signal transduction mechanisms in diabetes after stress may provide new insights into the development of diabetic neuropathy and neuropathic pain. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Neuroscience Letters
volume
515
issue
1
pages
34 - 38
publisher
Elsevier
external identifiers
  • wos:000303293600007
  • pmid:22446192
  • scopus:84859437979
ISSN
0304-3940
DOI
10.1016/j.neulet.2012.03.011
language
English
LU publication?
yes
id
76ee1119-b5b0-4c0d-aafd-0207f439a245 (old id 2431468)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22446192?dopt=Abstract
date added to LUP
2012-04-03 13:41:54
date last changed
2017-07-30 03:05:18
@article{76ee1119-b5b0-4c0d-aafd-0207f439a245,
  abstract     = {The aim of this study was to evaluate nerve regeneration in relation to the transcription factor, Activating Transcription Factor 3 (ATF 3), and an apoptotic marker, caspase 3, in the Schwann cells of diabetic BB rats (i.e. display type 1 diabetes phenotype). Sciatic nerves in healthy Wistar rats and in diabetic BB rats were transected and immediately repaired. Axonal outgrowth (neurofilament staining) and expression of ATF 3 and caspase 3 were quantified by immunohistochemistry after six days. There was no difference in axonal outgrowth between healthy and diabetic rats. However, the sciatic nerve in the diabetic rats exhibited a larger number of ATF 3 expressing Schwann cells at the site of the lesion and also a higher number of caspase 3 expressing Schwann cells. Similar differences were observed in the distal nerve segment between the healthy and diabetic rats. There were no correlations between the number of Schwann cells expressing ATF 3 and caspase 3. Thus, diabetic BB rats display an increased activation of ATF 3 and also a rise in apoptotic caspase 3 expressing Schwann cells, but with no discrepancy in length of axonal outgrowth after nerve injury and repair at six days. Knowledge about signal transduction mechanisms in diabetes after stress may provide new insights into the development of diabetic neuropathy and neuropathic pain.},
  author       = {Stenberg, Lena and Kanje, Martin and Dolezal, Katarina and Dahlin, Lars},
  issn         = {0304-3940},
  language     = {eng},
  number       = {1},
  pages        = {34--38},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Expression of Activating Transcription Factor 3 (ATF 3) and caspase 3 in Schwann cells and axonal outgrowth after sciatic nerve repair in diabetic BB rats.},
  url          = {http://dx.doi.org/10.1016/j.neulet.2012.03.011},
  volume       = {515},
  year         = {2012},
}