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Laccase catalysed oxidation of syringic acid: Calorimetric determination of kinetic parameters.

Volkova, Natalia LU ; Ibrahim, Victor LU and Hatti-Kaul, Rajni LU (2012) In Enzyme and Microbial Technology 50(4-5). p.233-237
Abstract
Isothermal titration calorimetry (ITC) was used to study the oxidation of syringic acid by laccases from two different sources: Galerina sp. HC1 and Trametes versicolor. Total molar heat of reaction with both enzymes was similar (230kJ/mol for Galerina laccase and 233kJ/mol for Trametes laccase), and was independent of syringic acid concentration. The kinetic parameters of the reaction were calculated from the single injection assay by applying the nonlinear least squares fitting (NLSF) of experimental data to the Michaelis-Menten equation. Higher values for V(max) were obtained with Galerina sp. laccase, whereas K(m) values were comparable for the two enzymes.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Enzyme and Microbial Technology
volume
50
issue
4-5
pages
233 - 237
publisher
Elsevier
external identifiers
  • wos:000303030500005
  • pmid:22418263
  • scopus:84858150006
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2012.01.005
language
English
LU publication?
yes
id
acd6b86f-3d4f-4f73-98d5-bdb518001361 (old id 2431824)
date added to LUP
2012-04-12 17:06:12
date last changed
2017-08-06 03:26:11
@article{acd6b86f-3d4f-4f73-98d5-bdb518001361,
  abstract     = {Isothermal titration calorimetry (ITC) was used to study the oxidation of syringic acid by laccases from two different sources: Galerina sp. HC1 and Trametes versicolor. Total molar heat of reaction with both enzymes was similar (230kJ/mol for Galerina laccase and 233kJ/mol for Trametes laccase), and was independent of syringic acid concentration. The kinetic parameters of the reaction were calculated from the single injection assay by applying the nonlinear least squares fitting (NLSF) of experimental data to the Michaelis-Menten equation. Higher values for V(max) were obtained with Galerina sp. laccase, whereas K(m) values were comparable for the two enzymes.},
  author       = {Volkova, Natalia and Ibrahim, Victor and Hatti-Kaul, Rajni},
  issn         = {0141-0229},
  language     = {eng},
  number       = {4-5},
  pages        = {233--237},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Laccase catalysed oxidation of syringic acid: Calorimetric determination of kinetic parameters.},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2012.01.005},
  volume       = {50},
  year         = {2012},
}