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Isolation of microbial natural products.

Sterner, Olov LU (2012) In Methods in Molecular Biology 864. p.393-413
Abstract
In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in which one is sure to find secondary metabolites of certain types, e.g., flavonoids, microbes can be expected to produce virtually anything and it is important to go about the fractionation procedure with an open mind. This chapter presents an... (More)
In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in which one is sure to find secondary metabolites of certain types, e.g., flavonoids, microbes can be expected to produce virtually anything and it is important to go about the fractionation procedure with an open mind. This chapter presents an overview of preparation of extracts from microbial sources, and various methods and strategies involved in the isolation and characterization of microbial natural products. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
864
pages
393 - 413
publisher
Springer
external identifiers
  • pmid:22367905
  • scopus:84859816386
ISSN
1940-6029
DOI
10.1007/978-1-61779-624-1_15
language
English
LU publication?
yes
id
b81ae55d-9634-4ae8-80fc-e3e957728de5 (old id 2432491)
date added to LUP
2012-04-04 11:51:13
date last changed
2017-01-01 03:44:23
@article{b81ae55d-9634-4ae8-80fc-e3e957728de5,
  abstract     = {In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in which one is sure to find secondary metabolites of certain types, e.g., flavonoids, microbes can be expected to produce virtually anything and it is important to go about the fractionation procedure with an open mind. This chapter presents an overview of preparation of extracts from microbial sources, and various methods and strategies involved in the isolation and characterization of microbial natural products.},
  author       = {Sterner, Olov},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {393--413},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Isolation of microbial natural products.},
  url          = {http://dx.doi.org/10.1007/978-1-61779-624-1_15},
  volume       = {864},
  year         = {2012},
}