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Characterization and localization of cysteine-rich secretory protein 3 (CRISP-3) in the human male reproductive tract

Udby, L; Bjartell, Anders LU ; Malm, Johan LU ; Egesten, Arne LU ; Lundwall, Åke LU ; Cowland, J B; Borregaard, N and Kjeldsen, L (2005) In Journal of Andrology 26(3). p.333-342
Abstract
Mammalian members of the cysteine-rich secretory protein (CRISP) family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, posttesticular sperm maturation, and capacitation to oocyte-sperm fusion, and possibly also penetration of the zona pellucida. Rodents express only 2 CRISPs (CRISP-1 and CRISP-2) in their male reproductive system, whereas humans and horses express an additional third member named CRISP-3. We have previously demonstrated that this protein is present in human seminal plasma as well as in other exocrine secretions, in blood plasma, and in neutrophilic granulocytes. To characterize the protein in seminal plasma and localize the production of... (More)
Mammalian members of the cysteine-rich secretory protein (CRISP) family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, posttesticular sperm maturation, and capacitation to oocyte-sperm fusion, and possibly also penetration of the zona pellucida. Rodents express only 2 CRISPs (CRISP-1 and CRISP-2) in their male reproductive system, whereas humans and horses express an additional third member named CRISP-3. We have previously demonstrated that this protein is present in human seminal plasma as well as in other exocrine secretions, in blood plasma, and in neutrophilic granulocytes. To characterize the protein in seminal plasma and localize the production of CRISP-3 in the human male reproductive tract, we performed immunoblotting and enzyme-linked immunosorbent assay measurements of seminal plasma and immunohistochemistry and in situ hybridization of tissue specimens. We were able to show that human CRISP-3 is a quantitatively minor seminal plasma protein not associated with prostasomes. Furthermore, CRISP-3 expression was found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. Examination of seminal plasma from vasectomized males indicates that organs downstream of the epididymis are probably the major sources of seminal plasma CRISP-3. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CRISP-2, CRISP-1, SGP28, semen, fertilization, prostate
in
Journal of Andrology
volume
26
issue
3
pages
333 - 342
publisher
American Society of Andrology
external identifiers
  • wos:000228934600008
  • scopus:18244387198
ISSN
0196-3635
DOI
10.2164/jandrol.04132
language
English
LU publication?
yes
id
3d9e1fcc-4364-4f95-b5d9-b5ce21ba62e9 (old id 243704)
date added to LUP
2007-08-08 10:23:23
date last changed
2017-11-05 04:26:50
@article{3d9e1fcc-4364-4f95-b5d9-b5ce21ba62e9,
  abstract     = {Mammalian members of the cysteine-rich secretory protein (CRISP) family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, posttesticular sperm maturation, and capacitation to oocyte-sperm fusion, and possibly also penetration of the zona pellucida. Rodents express only 2 CRISPs (CRISP-1 and CRISP-2) in their male reproductive system, whereas humans and horses express an additional third member named CRISP-3. We have previously demonstrated that this protein is present in human seminal plasma as well as in other exocrine secretions, in blood plasma, and in neutrophilic granulocytes. To characterize the protein in seminal plasma and localize the production of CRISP-3 in the human male reproductive tract, we performed immunoblotting and enzyme-linked immunosorbent assay measurements of seminal plasma and immunohistochemistry and in situ hybridization of tissue specimens. We were able to show that human CRISP-3 is a quantitatively minor seminal plasma protein not associated with prostasomes. Furthermore, CRISP-3 expression was found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. Examination of seminal plasma from vasectomized males indicates that organs downstream of the epididymis are probably the major sources of seminal plasma CRISP-3.},
  author       = {Udby, L and Bjartell, Anders and Malm, Johan and Egesten, Arne and Lundwall, Åke and Cowland, J B and Borregaard, N and Kjeldsen, L},
  issn         = {0196-3635},
  keyword      = {CRISP-2,CRISP-1,SGP28,semen,fertilization,prostate},
  language     = {eng},
  number       = {3},
  pages        = {333--342},
  publisher    = {American Society of Andrology},
  series       = {Journal of Andrology},
  title        = {Characterization and localization of cysteine-rich secretory protein 3 (CRISP-3) in the human male reproductive tract},
  url          = {http://dx.doi.org/10.2164/jandrol.04132},
  volume       = {26},
  year         = {2005},
}