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Immuno-affinity interactions in sample preparation and analysis

Tudorache, Madalina LU (2005)
Abstract
Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. environment, foodstuffs, clinical, pharmaceutical, diagnosis, etc.). Recently, the immuno-affinity interaction was exploited also in the sample preparation area, leading to techniques such as immuno-affinity extraction, immuno-affinity precipitation and immuno-affinity filtration, and a substantial development of an area that for a long time was neglected.



In this context, a novel immuno-affinity based sample... (More)
Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. environment, foodstuffs, clinical, pharmaceutical, diagnosis, etc.). Recently, the immuno-affinity interaction was exploited also in the sample preparation area, leading to techniques such as immuno-affinity extraction, immuno-affinity precipitation and immuno-affinity filtration, and a substantial development of an area that for a long time was neglected.



In this context, a novel immuno-affinity based sample preparation technique called Immuno Supported Liquid Membrane (ISLM) extraction is presented in this thesis. The technique allows sample cleanup and analyte enrichment in a single device that comprises both the extraction and immunoassay (imuno extraction-assay). The thesis details the development, optimisation as well as implementation aspects of the ISLM extraction, demonstrating the versatility of the technique. (Less)
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author
opponent
  • Professor Doctor Hennion, Marie-Claire, ESPCI (Ecole SupĂ©rieure de Physqiue et de Chimie de Paris), Laboratoire Environnement et Chimie Anal
organization
publishing date
type
Thesis
publication status
published
subject
keywords
immuno supported liquid membrane (ISLM) extraction, sample preparation, immunoassay, immuno-affinity interaction, antibody, Analytical chemistry, Analytisk kemi
pages
156 pages
publisher
Analytical Chemistry, Lund University
defense location
Centre for Chemistry and Chemical Engineering Sölvegatan 39 Lund
defense date
2005-09-02 10:30
ISBN
91-7422-089-6
language
English
LU publication?
yes
id
6d4d19a0-38be-44a5-ad11-2dc98c208b40 (old id 24391)
date added to LUP
2007-06-01 09:06:12
date last changed
2016-09-19 08:45:07
@phdthesis{6d4d19a0-38be-44a5-ad11-2dc98c208b40,
  abstract     = {Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. environment, foodstuffs, clinical, pharmaceutical, diagnosis, etc.). Recently, the immuno-affinity interaction was exploited also in the sample preparation area, leading to techniques such as immuno-affinity extraction, immuno-affinity precipitation and immuno-affinity filtration, and a substantial development of an area that for a long time was neglected.<br/><br>
<br/><br>
In this context, a novel immuno-affinity based sample preparation technique called Immuno Supported Liquid Membrane (ISLM) extraction is presented in this thesis. The technique allows sample cleanup and analyte enrichment in a single device that comprises both the extraction and immunoassay (imuno extraction-assay). The thesis details the development, optimisation as well as implementation aspects of the ISLM extraction, demonstrating the versatility of the technique.},
  author       = {Tudorache, Madalina},
  isbn         = {91-7422-089-6},
  keyword      = {immuno supported liquid membrane (ISLM) extraction,sample preparation,immunoassay,immuno-affinity interaction,antibody,Analytical chemistry,Analytisk kemi},
  language     = {eng},
  pages        = {156},
  publisher    = {Analytical Chemistry, Lund University},
  school       = {Lund University},
  title        = {Immuno-affinity interactions in sample preparation and analysis},
  year         = {2005},
}