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Dynamics of FtsZ assembly during sporulation in Streptomyces coelicolor A3(2)

Grantcharova, N; Lustig, U and Flärdh, Klas LU (2005) In Journal of Bacteriology 187(9). p.3227-3237
Abstract
FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized septation that converts sporogenic aerial hyphae into spores. The latter developmentally controlled septation involves the coordinated assembly of large numbers of FtsZ rings in each sporulating hyphal cell. We used an FtsZ-enhanced green fluorescent protein (EGFP) translational fusion to visualize the progression of FtsZ ring assembly in vivo during sporulation of aerial hyphae. This revealed that the regular placement of... (More)
FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized septation that converts sporogenic aerial hyphae into spores. The latter developmentally controlled septation involves the coordinated assembly of large numbers of FtsZ rings in each sporulating hyphal cell. We used an FtsZ-enhanced green fluorescent protein (EGFP) translational fusion to visualize the progression of FtsZ ring assembly in vivo during sporulation of aerial hyphae. This revealed that the regular placement of multiple FtsZ rings and initiation of cytokinesis was preceded by a protracted phase during which spiral-shaped FtsZ intermediates were detected along the length of the aerial hyphal cell. Time course experiments indicated that they were remodeled and gradually replaced by regularly spaced FtsZ rings. Such spiral-shaped filaments could also be detected with immunofluorescence microscopy using an antiserum against FtsZ. Based on our observations, we propose a model for the progression of Z-ring assembly during sporulation of S. coelicolor. Furthermore, mutants lacking the developmental regulatory genes whi4, whiB, whiG, whiH, and whiI were investigated. They failed in up-regulation of the expression of FtsZ-EGFP in aerial hyphae, which is consistent with the known effects of these genes on ftsZ transcription. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
187
issue
9
pages
3227 - 3237
publisher
American Society for Microbiology
external identifiers
  • pmid:15838050
  • wos:000228633800033
  • scopus:17644427311
ISSN
0021-9193
DOI
10.1128/JB.187.9.3227-3237.2005
language
English
LU publication?
no
id
a6b4ffbd-5ded-4820-b016-ca8e02a918bb (old id 244428)
date added to LUP
2007-08-08 15:03:02
date last changed
2017-08-06 03:33:40
@article{a6b4ffbd-5ded-4820-b016-ca8e02a918bb,
  abstract     = {FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized septation that converts sporogenic aerial hyphae into spores. The latter developmentally controlled septation involves the coordinated assembly of large numbers of FtsZ rings in each sporulating hyphal cell. We used an FtsZ-enhanced green fluorescent protein (EGFP) translational fusion to visualize the progression of FtsZ ring assembly in vivo during sporulation of aerial hyphae. This revealed that the regular placement of multiple FtsZ rings and initiation of cytokinesis was preceded by a protracted phase during which spiral-shaped FtsZ intermediates were detected along the length of the aerial hyphal cell. Time course experiments indicated that they were remodeled and gradually replaced by regularly spaced FtsZ rings. Such spiral-shaped filaments could also be detected with immunofluorescence microscopy using an antiserum against FtsZ. Based on our observations, we propose a model for the progression of Z-ring assembly during sporulation of S. coelicolor. Furthermore, mutants lacking the developmental regulatory genes whi4, whiB, whiG, whiH, and whiI were investigated. They failed in up-regulation of the expression of FtsZ-EGFP in aerial hyphae, which is consistent with the known effects of these genes on ftsZ transcription.},
  author       = {Grantcharova, N and Lustig, U and Flärdh, Klas},
  issn         = {0021-9193},
  language     = {eng},
  number       = {9},
  pages        = {3227--3237},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {Dynamics of FtsZ assembly during sporulation in Streptomyces coelicolor A3(2)},
  url          = {http://dx.doi.org/10.1128/JB.187.9.3227-3237.2005},
  volume       = {187},
  year         = {2005},
}