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Metabolic activity of Glomus intraradices in Arum- and Paris-type arbuscular mycorrhizal colonization

van Aarle, Ingrid LU ; Cavagnaro, TR; Smith, SE; Smith, FA and Dickson, S (2005) In New Phytologist 166(2). p.611-618
Abstract
Colonization of two plant species by Glomus intraradices was studied to investigate the two morphological types (Arum and Paris), their symbiotic interfaces and metabolic activities. Root pieces and sections were stained to observe the colonization and metabolic activity of all mycorrhizal structures. There were no growth responses observed in the plants caused by mycorrhizal symbiosis. The two morphological types had a similar percentage of root colonized, but the Arum-type had higher metabolic activity. Most of the mycorrhizal structures (88%) showed succinate dehydrogenase activity; about half showed acid phosphatase activity; and a small percentage showed alkaline phosphatase activity. Phosphatase activity was highest in arbuscules and... (More)
Colonization of two plant species by Glomus intraradices was studied to investigate the two morphological types (Arum and Paris), their symbiotic interfaces and metabolic activities. Root pieces and sections were stained to observe the colonization and metabolic activity of all mycorrhizal structures. There were no growth responses observed in the plants caused by mycorrhizal symbiosis. The two morphological types had a similar percentage of root colonized, but the Arum-type had higher metabolic activity. Most of the mycorrhizal structures (88%) showed succinate dehydrogenase activity; about half showed acid phosphatase activity; and a small percentage showed alkaline phosphatase activity. Phosphatase activity was highest in arbuscules and low in intercellular hyphae in the Arum-type colonization. In the Paris-type, hyphal coils and arbusculate coils showed a similar intermediate percentage of phosphatase activity. We conclude that acid phosphatase is more important than alkaline phosphatase in both colonization types. We discuss the possibility that, whereas arbuscules in Arum-type are the main site for phosphorus release to the host plant, both the hyphal and arbusculate coils may be involved in the Paris-type. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
activity, metabolic, LSCM (laser scanning confocal microscope), fluorescence), ELF (enzyme-labelled, coil, arbuscular mycorrhiza, arbuscule, phosphatase, succinate dehydrogenase
in
New Phytologist
volume
166
issue
2
pages
611 - 618
publisher
Wiley-Blackwell
external identifiers
  • pmid:15819923
  • wos:000228178900026
  • scopus:17644372025
ISSN
1469-8137
DOI
10.1111/j.1469-8137.2005.01340.x
language
English
LU publication?
yes
id
46daf481-66c9-47ca-af16-c3bbd0c1a399 (old id 246616)
date added to LUP
2007-08-20 10:36:01
date last changed
2017-07-16 03:31:02
@article{46daf481-66c9-47ca-af16-c3bbd0c1a399,
  abstract     = {Colonization of two plant species by Glomus intraradices was studied to investigate the two morphological types (Arum and Paris), their symbiotic interfaces and metabolic activities. Root pieces and sections were stained to observe the colonization and metabolic activity of all mycorrhizal structures. There were no growth responses observed in the plants caused by mycorrhizal symbiosis. The two morphological types had a similar percentage of root colonized, but the Arum-type had higher metabolic activity. Most of the mycorrhizal structures (88%) showed succinate dehydrogenase activity; about half showed acid phosphatase activity; and a small percentage showed alkaline phosphatase activity. Phosphatase activity was highest in arbuscules and low in intercellular hyphae in the Arum-type colonization. In the Paris-type, hyphal coils and arbusculate coils showed a similar intermediate percentage of phosphatase activity. We conclude that acid phosphatase is more important than alkaline phosphatase in both colonization types. We discuss the possibility that, whereas arbuscules in Arum-type are the main site for phosphorus release to the host plant, both the hyphal and arbusculate coils may be involved in the Paris-type.},
  author       = {van Aarle, Ingrid and Cavagnaro, TR and Smith, SE and Smith, FA and Dickson, S},
  issn         = {1469-8137},
  keyword      = {activity,metabolic,LSCM (laser scanning confocal microscope),fluorescence),ELF (enzyme-labelled,coil,arbuscular mycorrhiza,arbuscule,phosphatase,succinate dehydrogenase},
  language     = {eng},
  number       = {2},
  pages        = {611--618},
  publisher    = {Wiley-Blackwell},
  series       = {New Phytologist},
  title        = {Metabolic activity of Glomus intraradices in Arum- and Paris-type arbuscular mycorrhizal colonization},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2005.01340.x},
  volume       = {166},
  year         = {2005},
}