Advanced

Amphiregulin acts as an autocrine survival factor for adult sensory neurons

Nilsson, Anna LU and Kanje, Martin LU (2005) In NeuroReport 16(3). p.213-218
Abstract
We studied the effect of amphiregulin on axonal outgrowth and survival in sensory neurons in organ cultured and dissociated mouse dorsal root ganglia (DRG). Amphiregulin at 20 ng/ml stimulated axonal outgrowth in both preparations. The EGF receptor inhibitor AG1478 inhibited outgrowth at 10 mu M but not at 1 mu M, where it abolished the stimulatory effects of amphiregulin. Fluoro-Jade staining and neuronal counting showed that more neurons survived in culture in the presence of amphiregulin while AG1478 at 10 mu M but not 1 mu M increased cell death. Small and medium sized neurons were immunopositive for both amphiregulin and the EGF receptor. Taken together these results suggest that amphiregulin can act as an autocrine survival factor... (More)
We studied the effect of amphiregulin on axonal outgrowth and survival in sensory neurons in organ cultured and dissociated mouse dorsal root ganglia (DRG). Amphiregulin at 20 ng/ml stimulated axonal outgrowth in both preparations. The EGF receptor inhibitor AG1478 inhibited outgrowth at 10 mu M but not at 1 mu M, where it abolished the stimulatory effects of amphiregulin. Fluoro-Jade staining and neuronal counting showed that more neurons survived in culture in the presence of amphiregulin while AG1478 at 10 mu M but not 1 mu M increased cell death. Small and medium sized neurons were immunopositive for both amphiregulin and the EGF receptor. Taken together these results suggest that amphiregulin can act as an autocrine survival factor for sensory neurons and stimulate axonal outgrowth through the EGF receptor. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nerve growth factors, sensory neurons, nerve regeneration, receptor, EGF, spinal ganglia, axotomy, axons, amphiregulin, apoptosis
in
NeuroReport
volume
16
issue
3
pages
213 - 218
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:15706222
  • wos:000227620100002
  • scopus:14944350077
ISSN
1473-558X
language
English
LU publication?
yes
id
056c673f-01b1-4828-94c7-07e1e924f476 (old id 248890)
alternative location
http://www.neuroreport.com/pt/re/neuroreport/abstract.00001756-200502280-00002.htm;jsessionid=GzvSKJ42X01wp5BSvxfMGBKQ2Rh09WQTQ6DKjvGDJ9KCtZl81DkG!2112021004!181195629!8091!-1
date added to LUP
2007-08-03 15:36:41
date last changed
2017-10-22 03:40:39
@article{056c673f-01b1-4828-94c7-07e1e924f476,
  abstract     = {We studied the effect of amphiregulin on axonal outgrowth and survival in sensory neurons in organ cultured and dissociated mouse dorsal root ganglia (DRG). Amphiregulin at 20 ng/ml stimulated axonal outgrowth in both preparations. The EGF receptor inhibitor AG1478 inhibited outgrowth at 10 mu M but not at 1 mu M, where it abolished the stimulatory effects of amphiregulin. Fluoro-Jade staining and neuronal counting showed that more neurons survived in culture in the presence of amphiregulin while AG1478 at 10 mu M but not 1 mu M increased cell death. Small and medium sized neurons were immunopositive for both amphiregulin and the EGF receptor. Taken together these results suggest that amphiregulin can act as an autocrine survival factor for sensory neurons and stimulate axonal outgrowth through the EGF receptor.},
  author       = {Nilsson, Anna and Kanje, Martin},
  issn         = {1473-558X},
  keyword      = {nerve growth factors,sensory neurons,nerve regeneration,receptor,EGF,spinal ganglia,axotomy,axons,amphiregulin,apoptosis},
  language     = {eng},
  number       = {3},
  pages        = {213--218},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {Amphiregulin acts as an autocrine survival factor for adult sensory neurons},
  volume       = {16},
  year         = {2005},
}