Amphiregulin acts as an autocrine survival factor for adult sensory neurons
(2005) In NeuroReport 16(3). p.213-218- Abstract
- We studied the effect of amphiregulin on axonal outgrowth and survival in sensory neurons in organ cultured and dissociated mouse dorsal root ganglia (DRG). Amphiregulin at 20 ng/ml stimulated axonal outgrowth in both preparations. The EGF receptor inhibitor AG1478 inhibited outgrowth at 10 mu M but not at 1 mu M, where it abolished the stimulatory effects of amphiregulin. Fluoro-Jade staining and neuronal counting showed that more neurons survived in culture in the presence of amphiregulin while AG1478 at 10 mu M but not 1 mu M increased cell death. Small and medium sized neurons were immunopositive for both amphiregulin and the EGF receptor. Taken together these results suggest that amphiregulin can act as an autocrine survival factor... (More)
- We studied the effect of amphiregulin on axonal outgrowth and survival in sensory neurons in organ cultured and dissociated mouse dorsal root ganglia (DRG). Amphiregulin at 20 ng/ml stimulated axonal outgrowth in both preparations. The EGF receptor inhibitor AG1478 inhibited outgrowth at 10 mu M but not at 1 mu M, where it abolished the stimulatory effects of amphiregulin. Fluoro-Jade staining and neuronal counting showed that more neurons survived in culture in the presence of amphiregulin while AG1478 at 10 mu M but not 1 mu M increased cell death. Small and medium sized neurons were immunopositive for both amphiregulin and the EGF receptor. Taken together these results suggest that amphiregulin can act as an autocrine survival factor for sensory neurons and stimulate axonal outgrowth through the EGF receptor. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/248890
- author
- Nilsson, Anna LU and Kanje, Martin LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- nerve growth factors, sensory neurons, nerve regeneration, receptor, EGF, spinal ganglia, axotomy, axons, amphiregulin, apoptosis
- in
- NeuroReport
- volume
- 16
- issue
- 3
- pages
- 213 - 218
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:15706222
- wos:000227620100002
- scopus:14944350077
- ISSN
- 1473-558X
- language
- English
- LU publication?
- yes
- id
- 056c673f-01b1-4828-94c7-07e1e924f476 (old id 248890)
- alternative location
- http://www.neuroreport.com/pt/re/neuroreport/abstract.00001756-200502280-00002.htm;jsessionid=GzvSKJ42X01wp5BSvxfMGBKQ2Rh09WQTQ6DKjvGDJ9KCtZl81DkG!2112021004!181195629!8091!-1
- date added to LUP
- 2016-04-01 11:53:47
- date last changed
- 2022-01-26 19:48:24
@article{056c673f-01b1-4828-94c7-07e1e924f476, abstract = {{We studied the effect of amphiregulin on axonal outgrowth and survival in sensory neurons in organ cultured and dissociated mouse dorsal root ganglia (DRG). Amphiregulin at 20 ng/ml stimulated axonal outgrowth in both preparations. The EGF receptor inhibitor AG1478 inhibited outgrowth at 10 mu M but not at 1 mu M, where it abolished the stimulatory effects of amphiregulin. Fluoro-Jade staining and neuronal counting showed that more neurons survived in culture in the presence of amphiregulin while AG1478 at 10 mu M but not 1 mu M increased cell death. Small and medium sized neurons were immunopositive for both amphiregulin and the EGF receptor. Taken together these results suggest that amphiregulin can act as an autocrine survival factor for sensory neurons and stimulate axonal outgrowth through the EGF receptor.}}, author = {{Nilsson, Anna and Kanje, Martin}}, issn = {{1473-558X}}, keywords = {{nerve growth factors; sensory neurons; nerve regeneration; receptor; EGF; spinal ganglia; axotomy; axons; amphiregulin; apoptosis}}, language = {{eng}}, number = {{3}}, pages = {{213--218}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{NeuroReport}}, title = {{Amphiregulin acts as an autocrine survival factor for adult sensory neurons}}, url = {{http://www.neuroreport.com/pt/re/neuroreport/abstract.00001756-200502280-00002.htm;jsessionid=GzvSKJ42X01wp5BSvxfMGBKQ2Rh09WQTQ6DKjvGDJ9KCtZl81DkG!2112021004!181195629!8091!-1}}, volume = {{16}}, year = {{2005}}, }