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Real-time label-free affinity biosensors for enumeration of total bacteria based on immobilized concanavalin A

Jantra, Jongjit; Kanatharana, Proespichaya; Asawatreratanakul, Punnee; Hedström, Martin LU ; Mattiasson, Bo LU and Thavarungkul, Panote (2011) In Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering 46(13). p.1450-1460
Abstract
This work presents the results of the use of flow injection surface plasmon resonance and impedimetric affinity biosensors for detecting and enumerating total bacteria based on the binding between E. coli and Con A, immobilized on amodified gold electrode. The single analysis time for both techniques was less than 20 min. Dissociation between the immobilized Con A and the E. coli using 200 mM of glucose in HClt at pH of 2.00 enabling the sensor to be reused for between 29-35 times. Impedimetric detection provided a much lower limit of detection (12 CFU mL(-1)) than the surface plasmon resonance method (6.1 x 10(7) CFU mL(-1)). Using the impedimetric system, real sample analysis was performed and the results were compared to the plate count... (More)
This work presents the results of the use of flow injection surface plasmon resonance and impedimetric affinity biosensors for detecting and enumerating total bacteria based on the binding between E. coli and Con A, immobilized on amodified gold electrode. The single analysis time for both techniques was less than 20 min. Dissociation between the immobilized Con A and the E. coli using 200 mM of glucose in HClt at pH of 2.00 enabling the sensor to be reused for between 29-35 times. Impedimetric detection provided a much lower limit of detection (12 CFU mL(-1)) than the surface plasmon resonance method (6.1 x 10(7) CFU mL(-1)). Using the impedimetric system, real sample analysis was performed and the results were compared to the plate count agar method. Cell concentrations obtained by the biosensor were only slightly different from the result obtained from the plate count agar. The proposed system offers a rapid and useful tool for screening detection and enumeration of total bacteria. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacteria, surface plasmon resonance, impedance, biosensor, high, sensitivity, real-time
in
Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering
volume
46
issue
13
pages
1450 - 1460
publisher
Taylor & Francis
external identifiers
  • wos:000298320600002
  • scopus:80053548556
ISSN
1093-4529
DOI
10.1080/10934529.2011.609022
language
English
LU publication?
yes
id
d9f8fd80-ea28-4e7e-953d-aa4266822e55 (old id 2494078)
date added to LUP
2012-05-08 08:15:42
date last changed
2017-03-05 03:17:55
@article{d9f8fd80-ea28-4e7e-953d-aa4266822e55,
  abstract     = {This work presents the results of the use of flow injection surface plasmon resonance and impedimetric affinity biosensors for detecting and enumerating total bacteria based on the binding between E. coli and Con A, immobilized on amodified gold electrode. The single analysis time for both techniques was less than 20 min. Dissociation between the immobilized Con A and the E. coli using 200 mM of glucose in HClt at pH of 2.00 enabling the sensor to be reused for between 29-35 times. Impedimetric detection provided a much lower limit of detection (12 CFU mL(-1)) than the surface plasmon resonance method (6.1 x 10(7) CFU mL(-1)). Using the impedimetric system, real sample analysis was performed and the results were compared to the plate count agar method. Cell concentrations obtained by the biosensor were only slightly different from the result obtained from the plate count agar. The proposed system offers a rapid and useful tool for screening detection and enumeration of total bacteria.},
  author       = {Jantra, Jongjit and Kanatharana, Proespichaya and Asawatreratanakul, Punnee and Hedström, Martin and Mattiasson, Bo and Thavarungkul, Panote},
  issn         = {1093-4529},
  keyword      = {Bacteria,surface plasmon resonance,impedance,biosensor,high,sensitivity,real-time},
  language     = {eng},
  number       = {13},
  pages        = {1450--1460},
  publisher    = {Taylor & Francis},
  series       = {Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering},
  title        = {Real-time label-free affinity biosensors for enumeration of total bacteria based on immobilized concanavalin A},
  url          = {http://dx.doi.org/10.1080/10934529.2011.609022},
  volume       = {46},
  year         = {2011},
}