Advanced

Molecularly imprinted polymers targeting quercetin in high-temperature aqueous solutions

Pakade, Vusumzi; Lindahl, Sofia LU ; Chimuka, Luke and Turner, Charlotta LU (2012) In Journal of Chromatography A 1230. p.15-23
Abstract
Molecularly imprinted polymers (MIPs) targeting quercetin were prepared from 4-vinylpyridine and ethylene dimethacrylate (EDMA) under various solvent systems with the aim to form MIPs with high recognition for the quercetin molecule in aqueous systems at high temperature. A MIP prepared from the three-component solvent mixture of THF/H2O/MeOH showed potential in its application for the determination of quercetin in plants (onion). The polymer particles before and after washing were characterized by infrared spectroscopy and thermogravimetric analysis. Surface morphology was recorded by scanning electron microscopy. The binding capacity of the MIPs was investigated at 25 and 84 degrees C. respectively, in batch mode. Parameters, including... (More)
Molecularly imprinted polymers (MIPs) targeting quercetin were prepared from 4-vinylpyridine and ethylene dimethacrylate (EDMA) under various solvent systems with the aim to form MIPs with high recognition for the quercetin molecule in aqueous systems at high temperature. A MIP prepared from the three-component solvent mixture of THF/H2O/MeOH showed potential in its application for the determination of quercetin in plants (onion). The polymer particles before and after washing were characterized by infrared spectroscopy and thermogravimetric analysis. Surface morphology was recorded by scanning electron microscopy. The binding capacity of the MIPs was investigated at 25 and 84 degrees C. respectively, in batch mode. Parameters, including the influence of pH, extraction time and binding capacity, were evaluated. The slopes for the effect of extraction time revealed that the mass transfer of the analytes was higher at 84 degrees C than at 25 degrees C. Also, the binding capacity for the most promising MIP and its corresponding NIP was higher at 84 degrees C. The binding capacity for the MIP was similar to 30 mu mol g(-1) at 25 degrees C and similar to 120 mu mol g(-1) at 84 degrees C, while for the corresponding NIP, it was similar to 15 and similar to 90 mu mol g(-1), at 25 and 84 degrees C, respectively. A demonstration of MIP selectivity at higher temperature using standard solutions of selected flavonols showed that the MIP still retained its selectivity for quercetin. Similar selectivity was observed when preliminary application studies on aqueous yellow onion extracts were investigated. (C) 2012 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Extraction, High temperature, Molecular imprinting, Quercetin, Water, Yellow onion
in
Journal of Chromatography A
volume
1230
pages
15 - 23
publisher
Elsevier
external identifiers
  • wos:000301959700003
  • scopus:84857502367
ISSN
0021-9673
DOI
10.1016/j.chroma.2012.01.051
language
English
LU publication?
yes
id
62cd3acb-0d5c-471c-b2cb-9fdfac517764 (old id 2494823)
date added to LUP
2012-05-10 14:34:14
date last changed
2017-11-05 04:04:14
@article{62cd3acb-0d5c-471c-b2cb-9fdfac517764,
  abstract     = {Molecularly imprinted polymers (MIPs) targeting quercetin were prepared from 4-vinylpyridine and ethylene dimethacrylate (EDMA) under various solvent systems with the aim to form MIPs with high recognition for the quercetin molecule in aqueous systems at high temperature. A MIP prepared from the three-component solvent mixture of THF/H2O/MeOH showed potential in its application for the determination of quercetin in plants (onion). The polymer particles before and after washing were characterized by infrared spectroscopy and thermogravimetric analysis. Surface morphology was recorded by scanning electron microscopy. The binding capacity of the MIPs was investigated at 25 and 84 degrees C. respectively, in batch mode. Parameters, including the influence of pH, extraction time and binding capacity, were evaluated. The slopes for the effect of extraction time revealed that the mass transfer of the analytes was higher at 84 degrees C than at 25 degrees C. Also, the binding capacity for the most promising MIP and its corresponding NIP was higher at 84 degrees C. The binding capacity for the MIP was similar to 30 mu mol g(-1) at 25 degrees C and similar to 120 mu mol g(-1) at 84 degrees C, while for the corresponding NIP, it was similar to 15 and similar to 90 mu mol g(-1), at 25 and 84 degrees C, respectively. A demonstration of MIP selectivity at higher temperature using standard solutions of selected flavonols showed that the MIP still retained its selectivity for quercetin. Similar selectivity was observed when preliminary application studies on aqueous yellow onion extracts were investigated. (C) 2012 Elsevier B.V. All rights reserved.},
  author       = {Pakade, Vusumzi and Lindahl, Sofia and Chimuka, Luke and Turner, Charlotta},
  issn         = {0021-9673},
  keyword      = {Extraction,High temperature,Molecular imprinting,Quercetin,Water,Yellow onion},
  language     = {eng},
  pages        = {15--23},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Molecularly imprinted polymers targeting quercetin in high-temperature aqueous solutions},
  url          = {http://dx.doi.org/10.1016/j.chroma.2012.01.051},
  volume       = {1230},
  year         = {2012},
}