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Sol-Gel Derived Nanoporous Compositions for Entrapping Small Molecules and Their Outlook toward Aptamer Screening

Ahn, Ji-Young; Lee, Sang Wook LU ; Jo, Minjoung; Kang, Jeehye; Kim, Eunkyung; Jeong, Ok Chan; Laurell, Thomas LU and Kim, Soyoun (2012) In Analytical Chemistry 84(6). p.2647-2653
Abstract
This paper reports for the first time the application of sol-gel microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol-gel adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol-gel microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol-gel network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol-gel spots, as shown in microarrays forming the letters "L", "U", "N", and "D". Moreover, the bound aptamer was... (More)
This paper reports for the first time the application of sol-gel microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol-gel adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol-gel microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol-gel network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol-gel spots, as shown in microarrays forming the letters "L", "U", "N", and "D". Moreover, the bound aptamer was released by heat, recovered, and verified by gel electrophoresis. The developed PS-SG chip platform will be used for screening aptamers against numerous small molecules such as toxins, metabolites, or pesticide residues. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
84
issue
6
pages
2647 - 2653
publisher
The American Chemical Society
external identifiers
  • wos:000301634500006
  • scopus:84863342020
ISSN
1520-6882
DOI
10.1021/ac202559w
language
English
LU publication?
yes
id
8c70f0c5-2d3d-430d-99c0-19f53137cec0 (old id 2495066)
date added to LUP
2012-05-10 12:33:33
date last changed
2017-11-05 03:04:22
@article{8c70f0c5-2d3d-430d-99c0-19f53137cec0,
  abstract     = {This paper reports for the first time the application of sol-gel microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol-gel adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol-gel microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol-gel network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol-gel spots, as shown in microarrays forming the letters "L", "U", "N", and "D". Moreover, the bound aptamer was released by heat, recovered, and verified by gel electrophoresis. The developed PS-SG chip platform will be used for screening aptamers against numerous small molecules such as toxins, metabolites, or pesticide residues.},
  author       = {Ahn, Ji-Young and Lee, Sang Wook and Jo, Minjoung and Kang, Jeehye and Kim, Eunkyung and Jeong, Ok Chan and Laurell, Thomas and Kim, Soyoun},
  issn         = {1520-6882},
  language     = {eng},
  number       = {6},
  pages        = {2647--2653},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Sol-Gel Derived Nanoporous Compositions for Entrapping Small Molecules and Their Outlook toward Aptamer Screening},
  url          = {http://dx.doi.org/10.1021/ac202559w},
  volume       = {84},
  year         = {2012},
}