The neuroprotective effect of 2-oxoglutarate in the experimental ischemia of hippocampus
(2011) In Journal of Physiology and Pharmacology 62(2). p.239-246- Abstract
- In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding... (More)
- In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding protein S-100b to 1,6 times). Treatment with 2-oxoglutarate (2.28 g/l drinking water) for between 7 and 21 days attenuated the neuronal death and reactive astrogliosis in this model of experimental ischemia by 20-50%. Our results suggest that 2-OG may prevent the disturbances of neural cells that usually take place during ischemic pathology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2052554
- author
- Kovalenko, T. N. ; Ushakova, G. A. ; Osadchenko, I. ; Skibo, G. G. and Pierzynowski, Stefan LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 2-oxoglutarate, brain, glial fibrillary acid protein (GFAP), hippocampus, ischemia, neural cell adhesion molecule (NCAM), neuronal, specific nuclear protein (NeuN), S100 calcium binding protein B (S100b)
- in
- Journal of Physiology and Pharmacology
- volume
- 62
- issue
- 2
- pages
- 239 - 246
- publisher
- Polish Physiological Society
- external identifiers
-
- wos:000291769100012
- scopus:79959342918
- ISSN
- 0867-5910
- language
- English
- LU publication?
- yes
- id
- 24d4838c-3069-4e22-8635-857234d648e5 (old id 2052554)
- alternative location
- http://www.jpp.krakow.pl/journal/archive/04_11/pdf/239_04_11_article.pdf
- date added to LUP
- 2016-04-01 14:31:40
- date last changed
- 2022-03-22 00:29:15
@article{24d4838c-3069-4e22-8635-857234d648e5,
abstract = {{In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding protein S-100b to 1,6 times). Treatment with 2-oxoglutarate (2.28 g/l drinking water) for between 7 and 21 days attenuated the neuronal death and reactive astrogliosis in this model of experimental ischemia by 20-50%. Our results suggest that 2-OG may prevent the disturbances of neural cells that usually take place during ischemic pathology.}},
author = {{Kovalenko, T. N. and Ushakova, G. A. and Osadchenko, I. and Skibo, G. G. and Pierzynowski, Stefan}},
issn = {{0867-5910}},
keywords = {{2-oxoglutarate; brain; glial fibrillary acid protein (GFAP); hippocampus; ischemia; neural cell adhesion molecule (NCAM); neuronal; specific nuclear protein (NeuN); S100 calcium binding protein B (S100b)}},
language = {{eng}},
number = {{2}},
pages = {{239--246}},
publisher = {{Polish Physiological Society}},
series = {{Journal of Physiology and Pharmacology}},
title = {{The neuroprotective effect of 2-oxoglutarate in the experimental ischemia of hippocampus}},
url = {{http://www.jpp.krakow.pl/journal/archive/04_11/pdf/239_04_11_article.pdf}},
volume = {{62}},
year = {{2011}},
}