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Inhibitory effects of reserpine and carbonyl cyanide m-chloro-phenylhydrazone on fluoroquinolone resistance of Acinetobacter baumannii

Shi, WF; Jiang, JP; Xu, Ning LU ; Huang, ZM and Wang, YY (2005) In Chinese Medical Journal 118(4). p.340-343
Abstract
Mechanisms of bacterial resistance to fluoroquinolones may be grouped into three principal categories: gene mutations of DNA topoisomerase II (GyrA or GyrB), DNA topoisomerase IV (ParC or ParE), decrease of outer membrane permeation and upregulation of multi-drug efflux pump (active efflux system).(1) Efflux pumps are transport proteins removing toxic substrates ( including virtually all classes of clinically relevant antibiotics) from cells to the external environment. These proteins exist in both Gram positive bacteria and Gram negative bacteria as well as in fungi and mammalian (tumour) cells.(2-4) It has been reported that alkaloid reserpine and carbonyl cyanide m-chlorophenylhydrazone (CCCP) can inhibit NorA multi-drug efflux.(5,6) In... (More)
Mechanisms of bacterial resistance to fluoroquinolones may be grouped into three principal categories: gene mutations of DNA topoisomerase II (GyrA or GyrB), DNA topoisomerase IV (ParC or ParE), decrease of outer membrane permeation and upregulation of multi-drug efflux pump (active efflux system).(1) Efflux pumps are transport proteins removing toxic substrates ( including virtually all classes of clinically relevant antibiotics) from cells to the external environment. These proteins exist in both Gram positive bacteria and Gram negative bacteria as well as in fungi and mammalian (tumour) cells.(2-4) It has been reported that alkaloid reserpine and carbonyl cyanide m-chlorophenylhydrazone (CCCP) can inhibit NorA multi-drug efflux.(5,6) In order to explore the universality of drug efflux in microorganisms, 85 strains of Acinetobacter baumannii (A. baumannii) were tested using reserpine and CCCP. The quinolone-resistant-determining region (QRDR) of gyrA and parC genes in 35 isolates of A. baumannii were amplified by polymerase chain reaction (PCR) and sequenced by DNA sequencer. The correlation between resistant mutation regularity and bacterial drug efflux were analysed. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
m-chlorophenylhydrazone, carbonyl cyanide, reserpine, Acinetobacter baumanii, efflux effect, gyrA, parC
in
Chinese Medical Journal
volume
118
issue
4
pages
340 - 343
publisher
Chinese Medical Association
external identifiers
  • wos:000227511600014
  • pmid:15740676
ISSN
0366-6999
language
English
LU publication?
yes
id
0a6fdde9-b97b-41ae-b12c-438d2b1c3771 (old id 250031)
alternative location
http://www.cmj.org/Periodical/paperlist.asp?id=LW8227&linkintype=pubmed
date added to LUP
2007-08-15 15:33:05
date last changed
2016-04-16 03:48:36
@article{0a6fdde9-b97b-41ae-b12c-438d2b1c3771,
  abstract     = {Mechanisms of bacterial resistance to fluoroquinolones may be grouped into three principal categories: gene mutations of DNA topoisomerase II (GyrA or GyrB), DNA topoisomerase IV (ParC or ParE), decrease of outer membrane permeation and upregulation of multi-drug efflux pump (active efflux system).(1) Efflux pumps are transport proteins removing toxic substrates ( including virtually all classes of clinically relevant antibiotics) from cells to the external environment. These proteins exist in both Gram positive bacteria and Gram negative bacteria as well as in fungi and mammalian (tumour) cells.(2-4) It has been reported that alkaloid reserpine and carbonyl cyanide m-chlorophenylhydrazone (CCCP) can inhibit NorA multi-drug efflux.(5,6) In order to explore the universality of drug efflux in microorganisms, 85 strains of Acinetobacter baumannii (A. baumannii) were tested using reserpine and CCCP. The quinolone-resistant-determining region (QRDR) of gyrA and parC genes in 35 isolates of A. baumannii were amplified by polymerase chain reaction (PCR) and sequenced by DNA sequencer. The correlation between resistant mutation regularity and bacterial drug efflux were analysed.},
  author       = {Shi, WF and Jiang, JP and Xu, Ning and Huang, ZM and Wang, YY},
  issn         = {0366-6999},
  keyword      = {m-chlorophenylhydrazone,carbonyl cyanide,reserpine,Acinetobacter baumanii,efflux effect,gyrA,parC},
  language     = {eng},
  number       = {4},
  pages        = {340--343},
  publisher    = {Chinese Medical Association},
  series       = {Chinese Medical Journal},
  title        = {Inhibitory effects of reserpine and carbonyl cyanide m-chloro-phenylhydrazone on fluoroquinolone resistance of Acinetobacter baumannii},
  volume       = {118},
  year         = {2005},
}