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Single-laser shot fluorescence lifetime imaging on the nanosecond timescale using a Dual Image and Modeling Evaluation algorithm

Ehn, Andreas LU ; Johansson, Olof LU ; Arvidsson, Andreas LU ; Aldén, Marcus LU and Bood, Joakim LU (2012) In Optics Express 20(3). p.3043-3056
Abstract
A novel technique, designated dual imaging and modeling evaluation (DIME), for evaluating single-laser shot fluorescence lifetimes is presented. The technique is experimentally verified in a generic gas mixing experiment to provide a clear demonstration of the rapidness and sensitivity of the detector scheme. Single-laser shot fluorescence lifetimes of roughly 800 ps with a standard deviation of similar to 120 ps were determined. These results were compared to streak camera measurements. Furthermore, a general fluorescence lifetime determination algorithm is proposed. The evaluation algorithm has an analytic, linear relationship between the fluorescence lifetime and detector signal ratio. In combination with the DIME detector scheme, it is... (More)
A novel technique, designated dual imaging and modeling evaluation (DIME), for evaluating single-laser shot fluorescence lifetimes is presented. The technique is experimentally verified in a generic gas mixing experiment to provide a clear demonstration of the rapidness and sensitivity of the detector scheme. Single-laser shot fluorescence lifetimes of roughly 800 ps with a standard deviation of similar to 120 ps were determined. These results were compared to streak camera measurements. Furthermore, a general fluorescence lifetime determination algorithm is proposed. The evaluation algorithm has an analytic, linear relationship between the fluorescence lifetime and detector signal ratio. In combination with the DIME detector scheme, it is a faster, more accurate and more sensitive approach for rapid fluorescence lifetime imaging than previously proposed techniques. Monte Carlo simulations were conducted to analyze the sensitivity of the detector scheme as well as to compare the proposed evaluation algorithm to previously presented rapid lifetime determination algorithms. (C) 2012 Optical Society of America (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Optics Express
volume
20
issue
3
pages
3043 - 3056
publisher
OSA
external identifiers
  • wos:000300499500110
  • scopus:84856489312
ISSN
1094-4087
DOI
10.1364/OE.20.003043
language
English
LU publication?
yes
id
82c95543-a380-4ce6-8826-00896e6551d2 (old id 2517441)
date added to LUP
2012-05-15 07:44:39
date last changed
2017-11-19 04:02:09
@article{82c95543-a380-4ce6-8826-00896e6551d2,
  abstract     = {A novel technique, designated dual imaging and modeling evaluation (DIME), for evaluating single-laser shot fluorescence lifetimes is presented. The technique is experimentally verified in a generic gas mixing experiment to provide a clear demonstration of the rapidness and sensitivity of the detector scheme. Single-laser shot fluorescence lifetimes of roughly 800 ps with a standard deviation of similar to 120 ps were determined. These results were compared to streak camera measurements. Furthermore, a general fluorescence lifetime determination algorithm is proposed. The evaluation algorithm has an analytic, linear relationship between the fluorescence lifetime and detector signal ratio. In combination with the DIME detector scheme, it is a faster, more accurate and more sensitive approach for rapid fluorescence lifetime imaging than previously proposed techniques. Monte Carlo simulations were conducted to analyze the sensitivity of the detector scheme as well as to compare the proposed evaluation algorithm to previously presented rapid lifetime determination algorithms. (C) 2012 Optical Society of America},
  author       = {Ehn, Andreas and Johansson, Olof and Arvidsson, Andreas and Aldén, Marcus and Bood, Joakim},
  issn         = {1094-4087},
  language     = {eng},
  number       = {3},
  pages        = {3043--3056},
  publisher    = {OSA},
  series       = {Optics Express},
  title        = {Single-laser shot fluorescence lifetime imaging on the nanosecond timescale using a Dual Image and Modeling Evaluation algorithm},
  url          = {http://dx.doi.org/10.1364/OE.20.003043},
  volume       = {20},
  year         = {2012},
}