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Physical activity is associated with a large number of cardiovascular-specific proteins : Cross-sectional analyses in two independent cohorts

Stattin, Karl ; Lind, Lars ; Elmståhl, Sölve LU ; Wolk, Alicja ; Lemming, Eva Warensjö ; Melhus, Håkan ; Michaëlsson, Karl and Byberg, Liisa (2019) In European Journal of Preventive Cardiology
Abstract

Aims: We aimed to discover and replicate associations between leisure-time physical activity and cardiovascular candidate plasma protein biomarkers and to examine whether the associations were independent of body fat. Methods: We used cross-sectional data from two population-based cohorts, the EpiHealth (discovery cohort; n = 2239) and the Swedish Mammography Cohort – Clinical (SMCC; replication cohort; n = 4320). Physical activity during leisure time was assessed using questionnaires, and plasma concentrations of 184 proteins were assayed using the Olink Proseek Multiplex Cardiovascular 2 and 3 kits. We applied adjusted linear regression models using the False Discovery Rate to control for multiple testing in discovery. Results: In... (More)

Aims: We aimed to discover and replicate associations between leisure-time physical activity and cardiovascular candidate plasma protein biomarkers and to examine whether the associations were independent of body fat. Methods: We used cross-sectional data from two population-based cohorts, the EpiHealth (discovery cohort; n = 2239) and the Swedish Mammography Cohort – Clinical (SMCC; replication cohort; n = 4320). Physical activity during leisure time was assessed using questionnaires, and plasma concentrations of 184 proteins were assayed using the Olink Proseek Multiplex Cardiovascular 2 and 3 kits. We applied adjusted linear regression models using the False Discovery Rate to control for multiple testing in discovery. Results: In EpiHealth, physical activity was associated with 75 cardiovascular plasma biomarkers, of which 28 associations were verified (replicated) in SMCC. Findings include seven novel associations in human: paraoxonase 3, cystatin B, cathepsin Z, alpha-L-iduronidase, prostasin, growth differentiation factor 2 and tumour necrosis factor alpha receptor superfamily member 11A. Estimates for associations were similar across tertiles of body fat and physical activity was associated with four biomarkers independent of body fat percentage: paraoxonase 3, cystatin B, fatty acid-binding protein 4 and interleukin-1 receptor antagonist. Conclusion: Leisure-time physical activity was associated with 28 cardiovascular-specific proteins; four associations were independent of body fat. Biomarkers in novel associations are involved in several atherosclerotic processes including regulation of low-density lipoprotein oxidation, protein degradation and immune cell adhesion and migration. Further research into these pathways may yield new insights into how physical activity affects cardiovascular health.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
biomarkers, cardiovascular disease, exercise, Physical activity, proteins, proteomics
in
European Journal of Preventive Cardiology
publisher
SAGE Publications Ltd STM
external identifiers
  • pmid:31409108
  • scopus:85071488415
ISSN
2047-4873
DOI
10.1177/2047487319868033
language
English
LU publication?
yes
id
2517755c-4831-469c-a2d3-e5bd94b67965
date added to LUP
2019-09-23 11:34:57
date last changed
2019-12-10 08:15:22
@article{2517755c-4831-469c-a2d3-e5bd94b67965,
  abstract     = {<p>Aims: We aimed to discover and replicate associations between leisure-time physical activity and cardiovascular candidate plasma protein biomarkers and to examine whether the associations were independent of body fat. Methods: We used cross-sectional data from two population-based cohorts, the EpiHealth (discovery cohort; n = 2239) and the Swedish Mammography Cohort – Clinical (SMCC; replication cohort; n = 4320). Physical activity during leisure time was assessed using questionnaires, and plasma concentrations of 184 proteins were assayed using the Olink Proseek Multiplex Cardiovascular 2 and 3 kits. We applied adjusted linear regression models using the False Discovery Rate to control for multiple testing in discovery. Results: In EpiHealth, physical activity was associated with 75 cardiovascular plasma biomarkers, of which 28 associations were verified (replicated) in SMCC. Findings include seven novel associations in human: paraoxonase 3, cystatin B, cathepsin Z, alpha-L-iduronidase, prostasin, growth differentiation factor 2 and tumour necrosis factor alpha receptor superfamily member 11A. Estimates for associations were similar across tertiles of body fat and physical activity was associated with four biomarkers independent of body fat percentage: paraoxonase 3, cystatin B, fatty acid-binding protein 4 and interleukin-1 receptor antagonist. Conclusion: Leisure-time physical activity was associated with 28 cardiovascular-specific proteins; four associations were independent of body fat. Biomarkers in novel associations are involved in several atherosclerotic processes including regulation of low-density lipoprotein oxidation, protein degradation and immune cell adhesion and migration. Further research into these pathways may yield new insights into how physical activity affects cardiovascular health.</p>},
  author       = {Stattin, Karl and Lind, Lars and Elmståhl, Sölve and Wolk, Alicja and Lemming, Eva Warensjö and Melhus, Håkan and Michaëlsson, Karl and Byberg, Liisa},
  issn         = {2047-4873},
  language     = {eng},
  publisher    = {SAGE Publications Ltd STM},
  series       = {European Journal of Preventive Cardiology},
  title        = {Physical activity is associated with a large number of cardiovascular-specific proteins : Cross-sectional analyses in two independent cohorts},
  url          = {http://dx.doi.org/10.1177/2047487319868033},
  doi          = {10.1177/2047487319868033},
  year         = {2019},
}