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Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis.

Gustafsson, Rita LU ; Ohlsson, Bodil LU ; Benoni, Cecilia LU ; Jeppsson, Bengt LU and Olsson, Crister LU (2012) In World Journal of Gastroenterology 18(14). p.1628-1634
Abstract
AIM:

To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.



METHODS:

Biopsies were taken from the ascending colon and the total DNA was extracted. Universal bacterial primers were used to amplify the bacterial 16S rRNA genes. The amplicons were then cloned into competent Escherichia coli cells. The clones were sequenced and identified by comparison to known sequences.



RESULTS:

The clones could be divided into 44 different phylotypes. The microbiota was dominated by Firmicutes and Bacteroidetes. Seven phylotypes were found in both patients and constituted 47.5% of the total number of clones. Of... (More)
AIM:

To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.



METHODS:

Biopsies were taken from the ascending colon and the total DNA was extracted. Universal bacterial primers were used to amplify the bacterial 16S rRNA genes. The amplicons were then cloned into competent Escherichia coli cells. The clones were sequenced and identified by comparison to known sequences.



RESULTS:

The clones could be divided into 44 different phylotypes. The microbiota was dominated by Firmicutes and Bacteroidetes. Seven phylotypes were found in both patients and constituted 47.5% of the total number of clones. Of these, the most dominating were clones similar to Bacteroides cellulosilyticus, Bacteroides caccae, Bacteroides thetaiotaomicron, Bacteroides uniformis and Bacteroides dorei within Bacteroidetes. Sequences similar to Faecalibacterium prausnitzii and Clostridium citroniae were also found in both patients.



CONCLUSION:

A predominance of potentially pathogenic Bacteroides spp., and the presence of clones showing similarity to Clostridium clostridioforme were found but the overall colon microbiota showed similarities to a healthy one. Etiologies for collagenous colitis other than an adverse bacterial flora must also be considered. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
World Journal of Gastroenterology
volume
18
issue
14
pages
1628 - 1634
publisher
WJG Press
external identifiers
  • wos:000302931000010
  • pmid:22529692
  • scopus:84859962671
ISSN
1007-9327
DOI
10.3748/wjg.v18.i14.1628
language
English
LU publication?
yes
id
85c27370-26bd-40af-a5df-83279da729a6 (old id 2519133)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22529692?dopt=Abstract
date added to LUP
2012-05-06 20:18:02
date last changed
2017-10-01 04:56:21
@article{85c27370-26bd-40af-a5df-83279da729a6,
  abstract     = {AIM:<br/><br>
To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.<br/><br>
<br/><br>
METHODS:<br/><br>
Biopsies were taken from the ascending colon and the total DNA was extracted. Universal bacterial primers were used to amplify the bacterial 16S rRNA genes. The amplicons were then cloned into competent Escherichia coli cells. The clones were sequenced and identified by comparison to known sequences.<br/><br>
<br/><br>
RESULTS:<br/><br>
The clones could be divided into 44 different phylotypes. The microbiota was dominated by Firmicutes and Bacteroidetes. Seven phylotypes were found in both patients and constituted 47.5% of the total number of clones. Of these, the most dominating were clones similar to Bacteroides cellulosilyticus, Bacteroides caccae, Bacteroides thetaiotaomicron, Bacteroides uniformis and Bacteroides dorei within Bacteroidetes. Sequences similar to Faecalibacterium prausnitzii and Clostridium citroniae were also found in both patients.<br/><br>
<br/><br>
CONCLUSION:<br/><br>
A predominance of potentially pathogenic Bacteroides spp., and the presence of clones showing similarity to Clostridium clostridioforme were found but the overall colon microbiota showed similarities to a healthy one. Etiologies for collagenous colitis other than an adverse bacterial flora must also be considered.},
  author       = {Gustafsson, Rita and Ohlsson, Bodil and Benoni, Cecilia and Jeppsson, Bengt and Olsson, Crister},
  issn         = {1007-9327},
  language     = {eng},
  number       = {14},
  pages        = {1628--1634},
  publisher    = {WJG Press},
  series       = {World Journal of Gastroenterology},
  title        = {Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis.},
  url          = {http://dx.doi.org/10.3748/wjg.v18.i14.1628},
  volume       = {18},
  year         = {2012},
}